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Simultaneous determination of 13 aminoglycoside residues in foods of animal origin by liquid chromatography-electrospray ionization tandem mass spectrometry with two consecutive solid-phase extraction steps.
J Chromatogr A. 2008 Oct 17; 1207(1-2):29-37.JC

Abstract

A confirmatory and quantitative method based on liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) has been developed for simultaneous determination of 13 aminoglycoside antibiotics in various samples. The aminoglycoside analytes were released and extracted from different matrices with 5% trichloroacetic acid. The influence of pH values on the solid-phase exaction (SPE) procedure has been studied. Due to different pK(a) values of the compounds, seven aminoglycosides (AGs) were quantitatively retained on Oasis HLB cartridges at pH<1 and then six aminoglycosides were retained at pH 8.5. Thus, the combination of two HLB SPE cartridges with different pH values was involved to simultaneously purify 13 aminoglycosides. The proposed two SPE steps produced high recovery yields for every aminoglycoside in five different matrices. The LC-MS/MS method was validated according to the European Union Commission directive 2002/657/EC. Good performance characteristics were obtained for recovery, precision, calibration curve, stability, specificity, decision limits (CCalpha) and detection capabilities (CCbeta) in different matrices. The optimized procedure has been successfully applied to real samples in our laboratories (n> or =200) for 1 year. It demonstrated that the new method was robust and useful for identification and quantification of 13 aminoglycosides residues in foods of animal origin.

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Authors+Show Affiliations

Zhu WX
Henan Entry-Exit Inspection and Quarantine Bureau of China, Zhengzhou 450003, China; Department of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
Yang JZ
No affiliation info available
Wei W
No affiliation info available
Liu YF
No affiliation info available
Zhang SS
No affiliation info available

MeSH

AminoglycosidesChromatography, High Pressure LiquidDrug ResiduesFood AnalysisSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18757060

Citation

Zhu, Wei-xia, et al. "Simultaneous Determination of 13 Aminoglycoside Residues in Foods of Animal Origin By Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry With Two Consecutive Solid-phase Extraction Steps." Journal of Chromatography. A, vol. 1207, no. 1-2, 2008, pp. 29-37.
Zhu WX, Yang JZ, Wei W, et al. Simultaneous determination of 13 aminoglycoside residues in foods of animal origin by liquid chromatography-electrospray ionization tandem mass spectrometry with two consecutive solid-phase extraction steps. J Chromatogr A. 2008;1207(1-2):29-37.
Zhu, W. X., Yang, J. Z., Wei, W., Liu, Y. F., & Zhang, S. S. (2008). Simultaneous determination of 13 aminoglycoside residues in foods of animal origin by liquid chromatography-electrospray ionization tandem mass spectrometry with two consecutive solid-phase extraction steps. Journal of Chromatography. A, 1207(1-2), 29-37. https://doi.org/10.1016/j.chroma.2008.08.033
Zhu WX, et al. Simultaneous Determination of 13 Aminoglycoside Residues in Foods of Animal Origin By Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry With Two Consecutive Solid-phase Extraction Steps. J Chromatogr A. 2008 Oct 17;1207(1-2):29-37. PubMed PMID: 18757060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of 13 aminoglycoside residues in foods of animal origin by liquid chromatography-electrospray ionization tandem mass spectrometry with two consecutive solid-phase extraction steps. AU - Zhu,Wei-xia, AU - Yang,Ji-zhou, AU - Wei,Wei, AU - Liu,Ya-feng, AU - Zhang,Shu-sheng, Y1 - 2008/08/14/ PY - 2008/04/29/received PY - 2008/07/31/revised PY - 2008/08/11/accepted PY - 2008/9/2/pubmed PY - 2008/12/17/medline PY - 2008/9/2/entrez SP - 29 EP - 37 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1207 IS - 1-2 N2 - A confirmatory and quantitative method based on liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) has been developed for simultaneous determination of 13 aminoglycoside antibiotics in various samples. The aminoglycoside analytes were released and extracted from different matrices with 5% trichloroacetic acid. The influence of pH values on the solid-phase exaction (SPE) procedure has been studied. Due to different pK(a) values of the compounds, seven aminoglycosides (AGs) were quantitatively retained on Oasis HLB cartridges at pH<1 and then six aminoglycosides were retained at pH 8.5. Thus, the combination of two HLB SPE cartridges with different pH values was involved to simultaneously purify 13 aminoglycosides. The proposed two SPE steps produced high recovery yields for every aminoglycoside in five different matrices. The LC-MS/MS method was validated according to the European Union Commission directive 2002/657/EC. Good performance characteristics were obtained for recovery, precision, calibration curve, stability, specificity, decision limits (CCalpha) and detection capabilities (CCbeta) in different matrices. The optimized procedure has been successfully applied to real samples in our laboratories (n> or =200) for 1 year. It demonstrated that the new method was robust and useful for identification and quantification of 13 aminoglycosides residues in foods of animal origin. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18757060/Simultaneous_determination_of_13_aminoglycoside_residues_in_foods_of_animal_origin_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_with_two_consecutive_solid_phase_extraction_steps_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01368-X DB - PRIME DP - Unbound Medicine ER -
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