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Consumption of wonderful variety pomegranate juice and extract by diabetic patients increases paraoxonase 1 association with high-density lipoprotein and stimulates its catalytic activities.
J Agric Food Chem. 2008 Sep 24; 56(18):8704-13.JA

Abstract

Association of paraoxonase 1 (PON1) with high-density lipoprotein (HDL) stabilizes the enzyme. In diabetic patients, PON1 dissociates from HDL and, as a consequence, is less biologically active. Our aim was to investigate the effects of Wonderful variety pomegranate juice (WPJ) and pomegranate polyphenol extract (WPOMxl) consumption on PON1 association with HDL in diabetic patients. Thirty patients with type 2 diabetes mellitus participated in the study. Ten male patients and 10 female patients received concentrated WPJ (50 mL/day for 4 weeks), while another group of 10 male patients received WPOMxl (5 mL/day for 6 weeks). There were no significant effects of WPJ or WPOMxl consumption on fasting blood glucose or hemoglobin A1c levels. After 4 weeks of WPJ consumption by male patients, basal serum oxidative stress was significantly decreased by 35%, whereas serum concentrations of thiol groups significantly increased by 25%. Moreover, HDL-associated PON1 arylesterase, paraoxonase, and lactonase activities increased significantly after WPJ consumption by 34-45%, as compared to the baseline levels. PON1 protein binding to HDL was significantly increased by 30% following WPJ consumption, and the enzyme became more stable. In male patients that consumed WPOMxl and in female patients that consumed PJ, a similar pattern was observed, although to a lesser extent. We conclude that WPJ as well as WPOMxl consumption by diabetic patients does not worsen their diabetic parameters. Furthermore, WPJ as well as WPOMxl consumption contribute to PON1 stabilization, increased association with HDL, and enhanced catalytic activities. These beneficial effects of pomegranate consumption on serum PON1 stability and activity could lead to retardation of atherosclerosis development in diabetic patients.

Authors+Show Affiliations

Internal Medicine C Department, Ha'Emek Medical Center, Afula, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18759451

Citation

Rock, Wasseem, et al. "Consumption of Wonderful Variety Pomegranate Juice and Extract By Diabetic Patients Increases Paraoxonase 1 Association With High-density Lipoprotein and Stimulates Its Catalytic Activities." Journal of Agricultural and Food Chemistry, vol. 56, no. 18, 2008, pp. 8704-13.
Rock W, Rosenblat M, Miller-Lotan R, et al. Consumption of wonderful variety pomegranate juice and extract by diabetic patients increases paraoxonase 1 association with high-density lipoprotein and stimulates its catalytic activities. J Agric Food Chem. 2008;56(18):8704-13.
Rock, W., Rosenblat, M., Miller-Lotan, R., Levy, A. P., Elias, M., & Aviram, M. (2008). Consumption of wonderful variety pomegranate juice and extract by diabetic patients increases paraoxonase 1 association with high-density lipoprotein and stimulates its catalytic activities. Journal of Agricultural and Food Chemistry, 56(18), 8704-13. https://doi.org/10.1021/jf801756x
Rock W, et al. Consumption of Wonderful Variety Pomegranate Juice and Extract By Diabetic Patients Increases Paraoxonase 1 Association With High-density Lipoprotein and Stimulates Its Catalytic Activities. J Agric Food Chem. 2008 Sep 24;56(18):8704-13. PubMed PMID: 18759451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Consumption of wonderful variety pomegranate juice and extract by diabetic patients increases paraoxonase 1 association with high-density lipoprotein and stimulates its catalytic activities. AU - Rock,Wasseem, AU - Rosenblat,Mira, AU - Miller-Lotan,Rachel, AU - Levy,Andrew P, AU - Elias,Mazen, AU - Aviram,Michael, Y1 - 2008/08/30/ PY - 2008/9/2/pubmed PY - 2008/11/11/medline PY - 2008/9/2/entrez SP - 8704 EP - 13 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 56 IS - 18 N2 - Association of paraoxonase 1 (PON1) with high-density lipoprotein (HDL) stabilizes the enzyme. In diabetic patients, PON1 dissociates from HDL and, as a consequence, is less biologically active. Our aim was to investigate the effects of Wonderful variety pomegranate juice (WPJ) and pomegranate polyphenol extract (WPOMxl) consumption on PON1 association with HDL in diabetic patients. Thirty patients with type 2 diabetes mellitus participated in the study. Ten male patients and 10 female patients received concentrated WPJ (50 mL/day for 4 weeks), while another group of 10 male patients received WPOMxl (5 mL/day for 6 weeks). There were no significant effects of WPJ or WPOMxl consumption on fasting blood glucose or hemoglobin A1c levels. After 4 weeks of WPJ consumption by male patients, basal serum oxidative stress was significantly decreased by 35%, whereas serum concentrations of thiol groups significantly increased by 25%. Moreover, HDL-associated PON1 arylesterase, paraoxonase, and lactonase activities increased significantly after WPJ consumption by 34-45%, as compared to the baseline levels. PON1 protein binding to HDL was significantly increased by 30% following WPJ consumption, and the enzyme became more stable. In male patients that consumed WPOMxl and in female patients that consumed PJ, a similar pattern was observed, although to a lesser extent. We conclude that WPJ as well as WPOMxl consumption by diabetic patients does not worsen their diabetic parameters. Furthermore, WPJ as well as WPOMxl consumption contribute to PON1 stabilization, increased association with HDL, and enhanced catalytic activities. These beneficial effects of pomegranate consumption on serum PON1 stability and activity could lead to retardation of atherosclerosis development in diabetic patients. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/18759451/Consumption_of_wonderful_variety_pomegranate_juice_and_extract_by_diabetic_patients_increases_paraoxonase_1_association_with_high_density_lipoprotein_and_stimulates_its_catalytic_activities_ L2 - https://doi.org/10.1021/jf801756x DB - PRIME DP - Unbound Medicine ER -