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Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using signal DNA probe modified with Au and CuS nanoparticles.
Anal Chem. 2008 Oct 01; 80(19):7206-12.AC

Abstract

A novel and sensitive flow injection chemiluminescence assay for sequence-specific DNA detection based on signal amplification with nanoparticles (NPs) is reported in the present work. The "sandwich-type" DNA biosensor was fabricated with the thiol-functionalized capture DNA first immobilized on an Au electrode and hybridized with one end of target DNA, the other end of which was recognized with a signal DNA probe labeled with CuS NPs and Au NPs on the 3'- and 5'-terminus, respectively. The hybridization events were monitored by the CL intensity of luminol-H2O2-Cu(2+) after the cupric ions were dissolved from the hybrids. We demonstrated that the incorporation of Au NPs in this sensor design significantly enhanced the sensitivity and the selectivity because a single Au NP can be loaded with hundreds of signal DNA probe strands, which were modified with CuS NPs. The ratios of Au NPs, signal DNA probes, and CuS NPs modified on the gold electrode were approximately 1/101/103. A preconcentration process of cupric ions performed by anodic stripping voltammetry technology further increased the sensor performance. As a result of these two combined effects, this DNA sensor could detect as low as femtomolar target DNA and exhibited excellent selectivity against two-base mismatched DNA. Under the optimum conditions, the CL intensity was increased with the increase of the concentration of target DNA in the range of 2.0 x 10(-14)-2.0 x 10(-12) M. A detection limit of 4.8 x 10(-15) M target DNA was achieved.

Authors+Show Affiliations

Key Laboratory of Eco-chemical Engineering, Ministry of Education. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. shushzhang@126.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18759495

Citation

Zhang, Shusheng, et al. "Ultrasensitive Flow Injection Chemiluminescence Detection of DNA Hybridization Using Signal DNA Probe Modified With Au and CuS Nanoparticles." Analytical Chemistry, vol. 80, no. 19, 2008, pp. 7206-12.
Zhang S, Zhong H, Ding C. Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using signal DNA probe modified with Au and CuS nanoparticles. Anal Chem. 2008;80(19):7206-12.
Zhang, S., Zhong, H., & Ding, C. (2008). Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using signal DNA probe modified with Au and CuS nanoparticles. Analytical Chemistry, 80(19), 7206-12. https://doi.org/10.1021/ac800847r
Zhang S, Zhong H, Ding C. Ultrasensitive Flow Injection Chemiluminescence Detection of DNA Hybridization Using Signal DNA Probe Modified With Au and CuS Nanoparticles. Anal Chem. 2008 Oct 1;80(19):7206-12. PubMed PMID: 18759495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using signal DNA probe modified with Au and CuS nanoparticles. AU - Zhang,Shusheng, AU - Zhong,Hua, AU - Ding,Caifeng, Y1 - 2008/08/30/ PY - 2008/9/2/pubmed PY - 2008/12/17/medline PY - 2008/9/2/entrez SP - 7206 EP - 12 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 19 N2 - A novel and sensitive flow injection chemiluminescence assay for sequence-specific DNA detection based on signal amplification with nanoparticles (NPs) is reported in the present work. The "sandwich-type" DNA biosensor was fabricated with the thiol-functionalized capture DNA first immobilized on an Au electrode and hybridized with one end of target DNA, the other end of which was recognized with a signal DNA probe labeled with CuS NPs and Au NPs on the 3'- and 5'-terminus, respectively. The hybridization events were monitored by the CL intensity of luminol-H2O2-Cu(2+) after the cupric ions were dissolved from the hybrids. We demonstrated that the incorporation of Au NPs in this sensor design significantly enhanced the sensitivity and the selectivity because a single Au NP can be loaded with hundreds of signal DNA probe strands, which were modified with CuS NPs. The ratios of Au NPs, signal DNA probes, and CuS NPs modified on the gold electrode were approximately 1/101/103. A preconcentration process of cupric ions performed by anodic stripping voltammetry technology further increased the sensor performance. As a result of these two combined effects, this DNA sensor could detect as low as femtomolar target DNA and exhibited excellent selectivity against two-base mismatched DNA. Under the optimum conditions, the CL intensity was increased with the increase of the concentration of target DNA in the range of 2.0 x 10(-14)-2.0 x 10(-12) M. A detection limit of 4.8 x 10(-15) M target DNA was achieved. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/18759495/Ultrasensitive_flow_injection_chemiluminescence_detection_of_DNA_hybridization_using_signal_DNA_probe_modified_with_Au_and_CuS_nanoparticles_ L2 - https://doi.org/10.1021/ac800847r DB - PRIME DP - Unbound Medicine ER -