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Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver.
Aquat Toxicol. 2008 Sep 29; 89(4):242-50.AT

Abstract

The effects of acute perfluorododecanoic acid (PFDoA) exposure on the induction of oxidative stress and alteration of mitochondrial gene expression were studied in the livers of female zebrafish (Danio rerio). Female zebrafish were exposed to PFDoA via a single intraperitoneal injection (0, 20, 40, or 80 microg PFDoA/g body weight) and were then sacrificed 48 h, 96 h, or seven days post-PFDoA administration. PFDoA-treated fish exhibited histopathological liver damage, including swollen hepatocytes, vacuolar degeneration, and nuclei pycnosis. Glutathione (GSH) content and catalase (CAT) activity decreased significantly at 48 h post-injection while superoxide dismutase (SOD) activity was initially decreased at 48 h post-injection but was then elevated by seven days post-injection. The activity of glutathione peroxidase (GPx) increased at 48 h and seven days compared to control fish, although the increased level at seven days post-injection was decreased compared to the level at 48 h post-injection. Lipid peroxidation levels were increased at seven days post-injection, while no apparent induction was observed at 48 h or 96 h post-injection. The mRNA expression of medium-chain fatty acid dehydrogenase (MCAD) was induced, while the transcriptional expression of liver fatty acid binding protein (L-FABP), peroxisome proliferating activating receptor alpha (PPARalpha), carnitine palmitoyl-transferase I (CPT-I), uncoupling protein 2 (UCP-2), and Bcl-2 were significantly inhibited. Furthermore, the transcriptional expression of peroxisomal fatty acyl-CoA oxidase (ACOX), very long-chain acyl-CoA dehydrogenase (VLCAD), long-chain acyl-CoA dehydrogenase (LCAD) did not exhibit significant changes following PFDoA treatment. No significant changes were noted in the transcriptional expression of genes involved in mitochondrial respiratory chain and ATP synthesis, including cytochrome c oxidase subunit I (COXI), NADH dehydrogenase subunit I (NDI), and ATP synthase F0 subunit 6 (ATPo6). These results demonstrate that turbulence of fatty acid beta-oxidation and oxidative stress responses were involved in the PFDoA-induced hepatotoxicity.

Authors+Show Affiliations

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18760846

Citation

Liu, Yang, et al. "Induction of Time-dependent Oxidative Stress and Related Transcriptional Effects of Perfluorododecanoic Acid in Zebrafish Liver." Aquatic Toxicology (Amsterdam, Netherlands), vol. 89, no. 4, 2008, pp. 242-50.
Liu Y, Wang J, Wei Y, et al. Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver. Aquat Toxicol. 2008;89(4):242-50.
Liu, Y., Wang, J., Wei, Y., Zhang, H., Xu, M., & Dai, J. (2008). Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver. Aquatic Toxicology (Amsterdam, Netherlands), 89(4), 242-50. https://doi.org/10.1016/j.aquatox.2008.07.009
Liu Y, et al. Induction of Time-dependent Oxidative Stress and Related Transcriptional Effects of Perfluorododecanoic Acid in Zebrafish Liver. Aquat Toxicol. 2008 Sep 29;89(4):242-50. PubMed PMID: 18760846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver. AU - Liu,Yang, AU - Wang,Jianshe, AU - Wei,Yanhong, AU - Zhang,Hongxia, AU - Xu,Muqi, AU - Dai,Jiayin, Y1 - 2008/07/25/ PY - 2008/04/17/received PY - 2008/07/05/revised PY - 2008/07/13/accepted PY - 2008/9/2/pubmed PY - 2008/12/25/medline PY - 2008/9/2/entrez SP - 242 EP - 50 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 89 IS - 4 N2 - The effects of acute perfluorododecanoic acid (PFDoA) exposure on the induction of oxidative stress and alteration of mitochondrial gene expression were studied in the livers of female zebrafish (Danio rerio). Female zebrafish were exposed to PFDoA via a single intraperitoneal injection (0, 20, 40, or 80 microg PFDoA/g body weight) and were then sacrificed 48 h, 96 h, or seven days post-PFDoA administration. PFDoA-treated fish exhibited histopathological liver damage, including swollen hepatocytes, vacuolar degeneration, and nuclei pycnosis. Glutathione (GSH) content and catalase (CAT) activity decreased significantly at 48 h post-injection while superoxide dismutase (SOD) activity was initially decreased at 48 h post-injection but was then elevated by seven days post-injection. The activity of glutathione peroxidase (GPx) increased at 48 h and seven days compared to control fish, although the increased level at seven days post-injection was decreased compared to the level at 48 h post-injection. Lipid peroxidation levels were increased at seven days post-injection, while no apparent induction was observed at 48 h or 96 h post-injection. The mRNA expression of medium-chain fatty acid dehydrogenase (MCAD) was induced, while the transcriptional expression of liver fatty acid binding protein (L-FABP), peroxisome proliferating activating receptor alpha (PPARalpha), carnitine palmitoyl-transferase I (CPT-I), uncoupling protein 2 (UCP-2), and Bcl-2 were significantly inhibited. Furthermore, the transcriptional expression of peroxisomal fatty acyl-CoA oxidase (ACOX), very long-chain acyl-CoA dehydrogenase (VLCAD), long-chain acyl-CoA dehydrogenase (LCAD) did not exhibit significant changes following PFDoA treatment. No significant changes were noted in the transcriptional expression of genes involved in mitochondrial respiratory chain and ATP synthesis, including cytochrome c oxidase subunit I (COXI), NADH dehydrogenase subunit I (NDI), and ATP synthase F0 subunit 6 (ATPo6). These results demonstrate that turbulence of fatty acid beta-oxidation and oxidative stress responses were involved in the PFDoA-induced hepatotoxicity. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/18760846/Induction_of_time_dependent_oxidative_stress_and_related_transcriptional_effects_of_perfluorododecanoic_acid_in_zebrafish_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(08)00227-0 DB - PRIME DP - Unbound Medicine ER -