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Development and application of ultra performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry for determination of seven microcystins in water samples.
Anal Chim Acta. 2008 Sep 19; 626(1):28-36.AC

Abstract

In the present work, an ultra performance liquid chromatography (UPLC)-electrospray ionization tandem triple quadrupole/mass spectrometry (ESI-MS/MS) method was developed in selected reaction monitoring (SRM) mode to determine the amounts of seven/microcystin variants (MCYST-RR, -YR, -LR, -LA, -LY, -LW, -LF) in water samples. Solid phase extraction (SPE) or porous filter was used for sample cleaning-up and analyte enrichment. A C18 column enabled separation of 7 microcystins within 10min. Translation reaction ions including 995.6>134.9 (RR), 1045.8>135.1 (YR), 910.9>375.7 (LA), 1002.3>375.6 (LY), 520.1>135.0 (LR), 1025.8>891.7 (LW), 986.8>852.6 (LF) were set as quantitative ions and the enkephalin was the internal standard. Upon method validation, the limits of detection (LODs) and limits of quantification (LOQs) were found to be 0.06microgL(-1) and 0.2microgL(-1) (injection volume of 10microL), respectively, for all seven microcystin variants. The correlation coefficients were larger than 0.994 in all linear ranges and recoveries were in the range of 66.24-99.22%. The developed approach was highly sensitive and easy to perform. It generated accurate quantitative results for the analysis of microcystins in environmental water samples with a wide concentration range. The results showed that MC-RR and MC-LR widely existed in polluted water, while MC-LA and MC-LY were detected sporadically.

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Authors+Show Affiliations

Xu W
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital of Medical College, Zhejiang University 310003, China.
Chen Q
No affiliation info available
Zhang T
No affiliation info available
Cai Z
No affiliation info available
Jia X
No affiliation info available
Xie Q
No affiliation info available
Ren Y
No affiliation info available

MeSH

Chromatography, High Pressure LiquidMicrocystinsReference StandardsReproducibility of ResultsSensitivity and SpecificitySolid Phase ExtractionSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryTime FactorsWaterWater Pollutants, Chemical

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18761118

Citation

Xu, Wei, et al. "Development and Application of Ultra Performance Liquid Chromatography-electrospray Ionization Tandem Triple Quadrupole Mass Spectrometry for Determination of Seven Microcystins in Water Samples." Analytica Chimica Acta, vol. 626, no. 1, 2008, pp. 28-36.
Xu W, Chen Q, Zhang T, et al. Development and application of ultra performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry for determination of seven microcystins in water samples. Anal Chim Acta. 2008;626(1):28-36.
Xu, W., Chen, Q., Zhang, T., Cai, Z., Jia, X., Xie, Q., & Ren, Y. (2008). Development and application of ultra performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry for determination of seven microcystins in water samples. Analytica Chimica Acta, 626(1), 28-36. https://doi.org/10.1016/j.aca.2008.07.040
Xu W, et al. Development and Application of Ultra Performance Liquid Chromatography-electrospray Ionization Tandem Triple Quadrupole Mass Spectrometry for Determination of Seven Microcystins in Water Samples. Anal Chim Acta. 2008 Sep 19;626(1):28-36. PubMed PMID: 18761118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and application of ultra performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry for determination of seven microcystins in water samples. AU - Xu,Wei, AU - Chen,Qi, AU - Zhang,Ting, AU - Cai,Zengxuan, AU - Jia,Xiaofei, AU - Xie,Qing, AU - Ren,Yiping, Y1 - 2008/08/03/ PY - 2008/05/11/received PY - 2008/07/19/revised PY - 2008/07/21/accepted PY - 2008/9/2/pubmed PY - 2008/10/10/medline PY - 2008/9/2/entrez SP - 28 EP - 36 JF - Analytica chimica acta JO - Anal Chim Acta VL - 626 IS - 1 N2 - In the present work, an ultra performance liquid chromatography (UPLC)-electrospray ionization tandem triple quadrupole/mass spectrometry (ESI-MS/MS) method was developed in selected reaction monitoring (SRM) mode to determine the amounts of seven/microcystin variants (MCYST-RR, -YR, -LR, -LA, -LY, -LW, -LF) in water samples. Solid phase extraction (SPE) or porous filter was used for sample cleaning-up and analyte enrichment. A C18 column enabled separation of 7 microcystins within 10min. Translation reaction ions including 995.6>134.9 (RR), 1045.8>135.1 (YR), 910.9>375.7 (LA), 1002.3>375.6 (LY), 520.1>135.0 (LR), 1025.8>891.7 (LW), 986.8>852.6 (LF) were set as quantitative ions and the enkephalin was the internal standard. Upon method validation, the limits of detection (LODs) and limits of quantification (LOQs) were found to be 0.06microgL(-1) and 0.2microgL(-1) (injection volume of 10microL), respectively, for all seven microcystin variants. The correlation coefficients were larger than 0.994 in all linear ranges and recoveries were in the range of 66.24-99.22%. The developed approach was highly sensitive and easy to perform. It generated accurate quantitative results for the analysis of microcystins in environmental water samples with a wide concentration range. The results showed that MC-RR and MC-LR widely existed in polluted water, while MC-LA and MC-LY were detected sporadically. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/18761118/Development_and_application_of_ultra_performance_liquid_chromatography_electrospray_ionization_tandem_triple_quadrupole_mass_spectrometry_for_determination_of_seven_microcystins_in_water_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(08)01349-4 DB - PRIME DP - Unbound Medicine ER -
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