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Neurochemical pattern of the complex innervation of neuroepithelial bodies in mouse lungs.
Histochem Cell Biol 2009; 131(1):55-74HC

Abstract

As best characterized for rats, it is clear that pulmonary neuroepithelial bodies (NEBs) are contacted by a plethora of nerve fiber populations, suggesting that they represent an extensive group of multifunctional intraepithelial airway receptors. Because of the importance of genetically modified mice for functional studies, and the current lack of data, the main aim of the present study was to achieve a detailed analysis of the origin and neurochemical properties of nerve terminals associated with NEBs in mouse lungs. Antibodies against known selective markers for sensory and motor nerve terminals in rat lungs were used on lungs from control and vagotomized mice of two different strains, i.e., Swiss and C57-Bl6. NEB cells were visualized by antibodies against either the general neuroendocrine marker protein gene-product 9.5 (PGP9.5) or calcitonin gene-related peptide (CGRP). Thorough immunohistochemical examination of NEB cells showed that some of these NEB cells also exhibit calbindin D-28 k (CB) and vesicular acetylcholine transporter (VAChT) immunoreactivity (IR). Mouse pulmonary NEBs were found to receive intraepithelial nerve terminals of at least two different populations of myelinated vagal afferents: (1) Immunoreactive (ir) for vesicular glutamate transporters (VGLUTs) and CB; (2) expressing P2X(2) and P2X(3) ATP receptors. CGRP IR was seen in varicose vagal nerve fibers and in delicate non-vagal fibers, both in close proximity to NEBs. VAChT immunostaining showed very weak IR in the NEB-related intraepithelial vagal sensory nerve terminals. nNOS- or VIP-ir nerve terminals could be observed at the base of pulmonary NEBs. While a single NEB can be contacted by multiple nerve fiber populations, it was clear that none of the so far characterized nerve fiber populations contacts all pulmonary NEBs. The present study revealed that mouse lungs harbor several populations of nerve terminals that may selectively contact NEBs. Although at present the physiological significance of the innervation pattern of NEBs remains enigmatic, it is likely that NEBs are receptor-effector end-organs that may host complex and/or multiple functional properties in normal airways. The neurochemical information on the innervation of NEBs in mouse lungs gathered in the present study will be essential for the interpretation of upcoming functional data and for the study of transgenic mice.

Authors+Show Affiliations

Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Inge.Brouns@ua.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18762965

Citation

Brouns, Inge, et al. "Neurochemical Pattern of the Complex Innervation of Neuroepithelial Bodies in Mouse Lungs." Histochemistry and Cell Biology, vol. 131, no. 1, 2009, pp. 55-74.
Brouns I, Oztay F, Pintelon I, et al. Neurochemical pattern of the complex innervation of neuroepithelial bodies in mouse lungs. Histochem Cell Biol. 2009;131(1):55-74.
Brouns, I., Oztay, F., Pintelon, I., De Proost, I., Lembrechts, R., Timmermans, J. P., & Adriaensen, D. (2009). Neurochemical pattern of the complex innervation of neuroepithelial bodies in mouse lungs. Histochemistry and Cell Biology, 131(1), pp. 55-74. doi:10.1007/s00418-008-0495-7.
Brouns I, et al. Neurochemical Pattern of the Complex Innervation of Neuroepithelial Bodies in Mouse Lungs. Histochem Cell Biol. 2009;131(1):55-74. PubMed PMID: 18762965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurochemical pattern of the complex innervation of neuroepithelial bodies in mouse lungs. AU - Brouns,Inge, AU - Oztay,Fusun, AU - Pintelon,Isabel, AU - De Proost,Ian, AU - Lembrechts,Robrecht, AU - Timmermans,Jean-Pierre, AU - Adriaensen,Dirk, Y1 - 2008/09/02/ PY - 2008/08/12/accepted PY - 2008/9/3/pubmed PY - 2009/4/25/medline PY - 2008/9/3/entrez SP - 55 EP - 74 JF - Histochemistry and cell biology JO - Histochem. Cell Biol. VL - 131 IS - 1 N2 - As best characterized for rats, it is clear that pulmonary neuroepithelial bodies (NEBs) are contacted by a plethora of nerve fiber populations, suggesting that they represent an extensive group of multifunctional intraepithelial airway receptors. Because of the importance of genetically modified mice for functional studies, and the current lack of data, the main aim of the present study was to achieve a detailed analysis of the origin and neurochemical properties of nerve terminals associated with NEBs in mouse lungs. Antibodies against known selective markers for sensory and motor nerve terminals in rat lungs were used on lungs from control and vagotomized mice of two different strains, i.e., Swiss and C57-Bl6. NEB cells were visualized by antibodies against either the general neuroendocrine marker protein gene-product 9.5 (PGP9.5) or calcitonin gene-related peptide (CGRP). Thorough immunohistochemical examination of NEB cells showed that some of these NEB cells also exhibit calbindin D-28 k (CB) and vesicular acetylcholine transporter (VAChT) immunoreactivity (IR). Mouse pulmonary NEBs were found to receive intraepithelial nerve terminals of at least two different populations of myelinated vagal afferents: (1) Immunoreactive (ir) for vesicular glutamate transporters (VGLUTs) and CB; (2) expressing P2X(2) and P2X(3) ATP receptors. CGRP IR was seen in varicose vagal nerve fibers and in delicate non-vagal fibers, both in close proximity to NEBs. VAChT immunostaining showed very weak IR in the NEB-related intraepithelial vagal sensory nerve terminals. nNOS- or VIP-ir nerve terminals could be observed at the base of pulmonary NEBs. While a single NEB can be contacted by multiple nerve fiber populations, it was clear that none of the so far characterized nerve fiber populations contacts all pulmonary NEBs. The present study revealed that mouse lungs harbor several populations of nerve terminals that may selectively contact NEBs. Although at present the physiological significance of the innervation pattern of NEBs remains enigmatic, it is likely that NEBs are receptor-effector end-organs that may host complex and/or multiple functional properties in normal airways. The neurochemical information on the innervation of NEBs in mouse lungs gathered in the present study will be essential for the interpretation of upcoming functional data and for the study of transgenic mice. SN - 1432-119X UR - https://www.unboundmedicine.com/medline/citation/18762965/Neurochemical_pattern_of_the_complex_innervation_of_neuroepithelial_bodies_in_mouse_lungs_ L2 - https://dx.doi.org/10.1007/s00418-008-0495-7 DB - PRIME DP - Unbound Medicine ER -