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Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria).
ISME J. 2009 Jan; 3(1):31-46.IJ

Abstract

Pseudanabaena species are poorly known filamentous bloom-forming cyanobacteria closely related to Limnothrix. We isolated 28 Pseudanabaena strains from the Baltic Sea (BS) and the Albufera de Valencia (AV; Spain). By combining phenotypic and genotypic approaches, the phylogeny, diversity and evolutionary diversification of these isolates were explored. Analysis of the in vivo absorption spectra of the Pseudanabaena strains revealed two coexisting pigmentation phenotypes: (i) phycocyanin-rich (PC-rich) strains and (ii) strains containing both PC and phycoerythrin (PE). Strains of the latter phenotype were all capable of complementary chromatic adaptation (CCA). About 65 kb of the Pseudanabaena genomes were sequenced through a multilocus sequencing approach including the sequencing of the16 and 23S rRNA genes, the ribosomal intergenic spacer (IGS), internal transcribed spacer 1 (ITS-1), the cpcBA operon encoding PC and the IGS between cpcA and cpcB. In addition, the presence of nifH, one of the structural genes of nitrogenase, was investigated. Sequence analysis of ITS and cpcBA-IGS allowed the differentiation between Pseudanabaena isolates exhibiting high levels of microdiversity. This multilocus sequencing approach revealed specific clusters for the BS, the AV and a mixed cluster with strains from both ecosystems. The latter comprised exclusively CCA phenotypes. The phylogenies of the 16 and 23S rRNA genes are consistent, but analysis of other loci indicated the loss of substructure, suggesting that the recombination between these loci has occurred. Our preliminary results on population genetic analyses of the PC genes suggest an evolutionary diversification of Pseudanabaena through purifying selection.

Authors+Show Affiliations

Department of Marine Microbiology, Netherlands Institute of Ecology, NIOO-KNAW, AC Yerseke, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18769459

Citation

Acinas, Silvia G., et al. "Phenotypic and Genetic Diversification of Pseudanabaena Spp. (cyanobacteria)." The ISME Journal, vol. 3, no. 1, 2009, pp. 31-46.
Acinas SG, Haverkamp TH, Huisman J, et al. Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria). ISME J. 2009;3(1):31-46.
Acinas, S. G., Haverkamp, T. H., Huisman, J., & Stal, L. J. (2009). Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria). The ISME Journal, 3(1), 31-46. https://doi.org/10.1038/ismej.2008.78
Acinas SG, et al. Phenotypic and Genetic Diversification of Pseudanabaena Spp. (cyanobacteria). ISME J. 2009;3(1):31-46. PubMed PMID: 18769459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria). AU - Acinas,Silvia G, AU - Haverkamp,Thomas H A, AU - Huisman,Jef, AU - Stal,Lucas J, Y1 - 2008/09/04/ PY - 2008/9/5/pubmed PY - 2009/1/24/medline PY - 2008/9/5/entrez SP - 31 EP - 46 JF - The ISME journal JO - ISME J VL - 3 IS - 1 N2 - Pseudanabaena species are poorly known filamentous bloom-forming cyanobacteria closely related to Limnothrix. We isolated 28 Pseudanabaena strains from the Baltic Sea (BS) and the Albufera de Valencia (AV; Spain). By combining phenotypic and genotypic approaches, the phylogeny, diversity and evolutionary diversification of these isolates were explored. Analysis of the in vivo absorption spectra of the Pseudanabaena strains revealed two coexisting pigmentation phenotypes: (i) phycocyanin-rich (PC-rich) strains and (ii) strains containing both PC and phycoerythrin (PE). Strains of the latter phenotype were all capable of complementary chromatic adaptation (CCA). About 65 kb of the Pseudanabaena genomes were sequenced through a multilocus sequencing approach including the sequencing of the16 and 23S rRNA genes, the ribosomal intergenic spacer (IGS), internal transcribed spacer 1 (ITS-1), the cpcBA operon encoding PC and the IGS between cpcA and cpcB. In addition, the presence of nifH, one of the structural genes of nitrogenase, was investigated. Sequence analysis of ITS and cpcBA-IGS allowed the differentiation between Pseudanabaena isolates exhibiting high levels of microdiversity. This multilocus sequencing approach revealed specific clusters for the BS, the AV and a mixed cluster with strains from both ecosystems. The latter comprised exclusively CCA phenotypes. The phylogenies of the 16 and 23S rRNA genes are consistent, but analysis of other loci indicated the loss of substructure, suggesting that the recombination between these loci has occurred. Our preliminary results on population genetic analyses of the PC genes suggest an evolutionary diversification of Pseudanabaena through purifying selection. SN - 1751-7370 UR - https://www.unboundmedicine.com/medline/citation/18769459/Phenotypic_and_genetic_diversification_of_Pseudanabaena_spp___cyanobacteria__ DB - PRIME DP - Unbound Medicine ER -