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Sophocarpine and matrine inhibit the production of TNF-alpha and IL-6 in murine macrophages and prevent cachexia-related symptoms induced by colon26 adenocarcinoma in mice.
Int Immunopharmacol. 2008 Dec 20; 8(13-14):1767-72.II

Abstract

The present study aims to access the effects of sophora alkaloids on the production of pro-inflammatory cytokines and evaluate their therapeutic efficiency on cachexia. The comparative study showed that all sophora alkaloids tested here, including matrine, oxymatrine, sophocarpine, sophoramine, and sophoridine, inhibited TNF-alpha and IL-6 production in both RAW264.7 cells and murine primary macrophages, and sophocarpine showed the most potent inhibitory effect among them. Quantification of TNF-alpha and IL-6 mRNA in RAW264.7 cells by real-time RT-PCR revealed that both sophocarpine and matrine suppressed TNF-alpha and IL-6 expression and sophocarpine has stronger suppressing potency than matrine. Inoculation (s.c.) of colon26 adenocarcinoma cells into BALB/c mice induced cachexia, as evidenced by progressive weight loss, reduction in food intake, wasting of gastrocnemius muscle and epididymal fat, and increase in serum levels of TNF-alpha and IL-6. Administration of 50 mg/kg/d sophocarpine or matrine for 5 days from the onset of cachexia did not inhibit the tumor growth but resulted in attenuation of cachexia symptoms. Furthermore, sophocarpine and matrine decreased the serum levels of TNF-alpha and IL-6, and sophocarpine showed a better therapeutic effect than matrine. These results suggest that sophocarpine and matrine exert anti-cachectic effects probably through inhibition of TNF-alpha and IL-6.

Authors+Show Affiliations

Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18775799

Citation

Zhang, Yuefan, et al. "Sophocarpine and Matrine Inhibit the Production of TNF-alpha and IL-6 in Murine Macrophages and Prevent Cachexia-related Symptoms Induced By Colon26 Adenocarcinoma in Mice." International Immunopharmacology, vol. 8, no. 13-14, 2008, pp. 1767-72.
Zhang Y, Wang S, Li Y, et al. Sophocarpine and matrine inhibit the production of TNF-alpha and IL-6 in murine macrophages and prevent cachexia-related symptoms induced by colon26 adenocarcinoma in mice. Int Immunopharmacol. 2008;8(13-14):1767-72.
Zhang, Y., Wang, S., Li, Y., Xiao, Z., Hu, Z., & Zhang, J. (2008). Sophocarpine and matrine inhibit the production of TNF-alpha and IL-6 in murine macrophages and prevent cachexia-related symptoms induced by colon26 adenocarcinoma in mice. International Immunopharmacology, 8(13-14), 1767-72. https://doi.org/10.1016/j.intimp.2008.08.008
Zhang Y, et al. Sophocarpine and Matrine Inhibit the Production of TNF-alpha and IL-6 in Murine Macrophages and Prevent Cachexia-related Symptoms Induced By Colon26 Adenocarcinoma in Mice. Int Immunopharmacol. 2008 Dec 20;8(13-14):1767-72. PubMed PMID: 18775799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sophocarpine and matrine inhibit the production of TNF-alpha and IL-6 in murine macrophages and prevent cachexia-related symptoms induced by colon26 adenocarcinoma in mice. AU - Zhang,Yuefan, AU - Wang,Shaozhan, AU - Li,Yuanyuan, AU - Xiao,Zhenyu, AU - Hu,Zhenlin, AU - Zhang,Junping, Y1 - 2008/09/05/ PY - 2008/07/15/received PY - 2008/08/12/revised PY - 2008/08/18/accepted PY - 2008/9/9/pubmed PY - 2009/4/25/medline PY - 2008/9/9/entrez SP - 1767 EP - 72 JF - International immunopharmacology JO - Int Immunopharmacol VL - 8 IS - 13-14 N2 - The present study aims to access the effects of sophora alkaloids on the production of pro-inflammatory cytokines and evaluate their therapeutic efficiency on cachexia. The comparative study showed that all sophora alkaloids tested here, including matrine, oxymatrine, sophocarpine, sophoramine, and sophoridine, inhibited TNF-alpha and IL-6 production in both RAW264.7 cells and murine primary macrophages, and sophocarpine showed the most potent inhibitory effect among them. Quantification of TNF-alpha and IL-6 mRNA in RAW264.7 cells by real-time RT-PCR revealed that both sophocarpine and matrine suppressed TNF-alpha and IL-6 expression and sophocarpine has stronger suppressing potency than matrine. Inoculation (s.c.) of colon26 adenocarcinoma cells into BALB/c mice induced cachexia, as evidenced by progressive weight loss, reduction in food intake, wasting of gastrocnemius muscle and epididymal fat, and increase in serum levels of TNF-alpha and IL-6. Administration of 50 mg/kg/d sophocarpine or matrine for 5 days from the onset of cachexia did not inhibit the tumor growth but resulted in attenuation of cachexia symptoms. Furthermore, sophocarpine and matrine decreased the serum levels of TNF-alpha and IL-6, and sophocarpine showed a better therapeutic effect than matrine. These results suggest that sophocarpine and matrine exert anti-cachectic effects probably through inhibition of TNF-alpha and IL-6. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/18775799/Sophocarpine_and_matrine_inhibit_the_production_of_TNF_alpha_and_IL_6_in_murine_macrophages_and_prevent_cachexia_related_symptoms_induced_by_colon26_adenocarcinoma_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(08)00250-6 DB - PRIME DP - Unbound Medicine ER -