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Gene expression of a novel defensin antimicrobial peptide in the silkworm, Bombyx mori.
Biosci Biotechnol Biochem. 2008 Sep; 72(9):2353-61.BB

Abstract

cDNA encoding a novel defensin (BmDefensinB) was cloned from the fat body of the silkworm, Bombyx mori, and gene expression was analyzed. BmDefensinB showed typical structural characteristics of invertebrate defensins. Phylogenetic and bootstrap analyses indicated that it has no orthologs, whereas previously reported BmDefensinA is the ortholog of Spodoptera frugiperda (Sf)Spodoptericin. The BmDefensinB gene was expressed tissue-specifically in the fat body and was strongly activated by bacteria such as Escherichia coli and Bacillus subtilis, and by an entomopathogenic fungus Beauveria bassiana. In contrast, the BmDefensinA gene was expressed to a much lesser extent. Expression of the BmDefensinB gene was strongly stimulated by B. mori Rel proteins RelB and Relish, supporting the observation that this gene is activated by E. coli, B. subtilis, and B. bassiana. These results suggest that BmDefensinB gene expression is controlled through both the Toll and the Imd pathway, and that this gene plays an important role in B. mori immune reactions against infection by bacteria and fungi.

Authors+Show Affiliations

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18776686

Citation

Kaneko, Yoichi, et al. "Gene Expression of a Novel Defensin Antimicrobial Peptide in the Silkworm, Bombyx Mori." Bioscience, Biotechnology, and Biochemistry, vol. 72, no. 9, 2008, pp. 2353-61.
Kaneko Y, Tanaka H, Ishibashi J, et al. Gene expression of a novel defensin antimicrobial peptide in the silkworm, Bombyx mori. Biosci Biotechnol Biochem. 2008;72(9):2353-61.
Kaneko, Y., Tanaka, H., Ishibashi, J., Iwasaki, T., & Yamakawa, M. (2008). Gene expression of a novel defensin antimicrobial peptide in the silkworm, Bombyx mori. Bioscience, Biotechnology, and Biochemistry, 72(9), 2353-61.
Kaneko Y, et al. Gene Expression of a Novel Defensin Antimicrobial Peptide in the Silkworm, Bombyx Mori. Biosci Biotechnol Biochem. 2008;72(9):2353-61. PubMed PMID: 18776686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression of a novel defensin antimicrobial peptide in the silkworm, Bombyx mori. AU - Kaneko,Yoichi, AU - Tanaka,Hiromitsu, AU - Ishibashi,Jun, AU - Iwasaki,Takashi, AU - Yamakawa,Minoru, Y1 - 2008/09/07/ PY - 2008/9/9/pubmed PY - 2008/12/19/medline PY - 2008/9/9/entrez SP - 2353 EP - 61 JF - Bioscience, biotechnology, and biochemistry JO - Biosci. Biotechnol. Biochem. VL - 72 IS - 9 N2 - cDNA encoding a novel defensin (BmDefensinB) was cloned from the fat body of the silkworm, Bombyx mori, and gene expression was analyzed. BmDefensinB showed typical structural characteristics of invertebrate defensins. Phylogenetic and bootstrap analyses indicated that it has no orthologs, whereas previously reported BmDefensinA is the ortholog of Spodoptera frugiperda (Sf)Spodoptericin. The BmDefensinB gene was expressed tissue-specifically in the fat body and was strongly activated by bacteria such as Escherichia coli and Bacillus subtilis, and by an entomopathogenic fungus Beauveria bassiana. In contrast, the BmDefensinA gene was expressed to a much lesser extent. Expression of the BmDefensinB gene was strongly stimulated by B. mori Rel proteins RelB and Relish, supporting the observation that this gene is activated by E. coli, B. subtilis, and B. bassiana. These results suggest that BmDefensinB gene expression is controlled through both the Toll and the Imd pathway, and that this gene plays an important role in B. mori immune reactions against infection by bacteria and fungi. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/18776686/Gene_expression_of_a_novel_defensin_antimicrobial_peptide_in_the_silkworm_Bombyx_mori_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/bbb/80263?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -