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Single drop microextraction using silver nanoparticles as electrostatic probes for peptide analysis in atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry and comparison with gold electrostatic probes and silver hydrophobic probes.
Rapid Commun Mass Spectrom. 2008 Oct; 22(19):3076-86.RC

Abstract

Single drop microextraction using tetraalkylammonium bromide coated silver nanoparticles (SDME-AgNPs) prepared in toluene has been successfully applied as electrostatic affinity probes to preconcentrate peptide mixtures in biological samples prior to atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry (AP-MALDI-MS) analysis. This approach is based on the isoelectric point (pI) of peptides and surface charge of AgNPs. Using the SDME-AgNPs technique, from a peptide mixture, Met- and Leu-enkephalins (Met-enk and Leu-enk) were extracted into a droplet of toluene containing AgNPs, but not the neutral peptides (gramicidins). The best peptide extraction efficiency for SDME-AgNPs was observed with the optimized parameters: extraction time 2 min, sample agitation rate 240 rpm, and sample pH 7. The limits of detection (LODs) of the SDME-AgNPs/AP-MALDI-MS technique for Met-enk and Leu-enk peptides were 160 and 210 nM, respectively. Furthermore, the application of the technique has been shown for the analysis of peptides from a sample containing high matrix interferences such as 1% Triton X-100 and 6 M urea. Finally, this approach has been compared with the SDME-AuNPs technique and the results have clearly revealed that the SDME-AgNP affinity probe exhibits higher affinity to extract the sulfur-bearing peptide (Met-enk). We also compared this electrostatic affinity probe of AgNPs with the previously demonstrated hydrophobic affinity probe of AgNPs and found that the electrostatic probe can greatly reduce the extraction time from 1.5 h to 2 min. This is due to the fact that electrostatic attraction forces are much stronger than the hydrophobic attraction forces. Therefore, we concluded that the electrostatic affinity probe based on SDME-AgNPs coupled with AP-MALDI-MS is a high-throughput technique for the analysis of low-abundance peptides from biological samples containing complex matrices.

Authors+Show Affiliations

Graduate Institute of Life Sciences, Tamkang University, Tamsui 251, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18777509

Citation

Sudhir, Putty-Reddy, et al. "Single Drop Microextraction Using Silver Nanoparticles as Electrostatic Probes for Peptide Analysis in Atmospheric Pressure Matrix-assisted Laser Desorption/ionization Mass Spectrometry and Comparison With Gold Electrostatic Probes and Silver Hydrophobic Probes." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 19, 2008, pp. 3076-86.
Sudhir PR, Shrivas K, Zhou ZC, et al. Single drop microextraction using silver nanoparticles as electrostatic probes for peptide analysis in atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry and comparison with gold electrostatic probes and silver hydrophobic probes. Rapid Commun Mass Spectrom. 2008;22(19):3076-86.
Sudhir, P. R., Shrivas, K., Zhou, Z. C., & Wu, H. F. (2008). Single drop microextraction using silver nanoparticles as electrostatic probes for peptide analysis in atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry and comparison with gold electrostatic probes and silver hydrophobic probes. Rapid Communications in Mass Spectrometry : RCM, 22(19), 3076-86. https://doi.org/10.1002/rcm.3710
Sudhir PR, et al. Single Drop Microextraction Using Silver Nanoparticles as Electrostatic Probes for Peptide Analysis in Atmospheric Pressure Matrix-assisted Laser Desorption/ionization Mass Spectrometry and Comparison With Gold Electrostatic Probes and Silver Hydrophobic Probes. Rapid Commun Mass Spectrom. 2008;22(19):3076-86. PubMed PMID: 18777509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single drop microextraction using silver nanoparticles as electrostatic probes for peptide analysis in atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry and comparison with gold electrostatic probes and silver hydrophobic probes. AU - Sudhir,Putty-Reddy, AU - Shrivas,Kamlesh, AU - Zhou,Zi-Cong, AU - Wu,Hui-Fen, PY - 2008/9/9/pubmed PY - 2008/11/19/medline PY - 2008/9/9/entrez SP - 3076 EP - 86 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 19 N2 - Single drop microextraction using tetraalkylammonium bromide coated silver nanoparticles (SDME-AgNPs) prepared in toluene has been successfully applied as electrostatic affinity probes to preconcentrate peptide mixtures in biological samples prior to atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry (AP-MALDI-MS) analysis. This approach is based on the isoelectric point (pI) of peptides and surface charge of AgNPs. Using the SDME-AgNPs technique, from a peptide mixture, Met- and Leu-enkephalins (Met-enk and Leu-enk) were extracted into a droplet of toluene containing AgNPs, but not the neutral peptides (gramicidins). The best peptide extraction efficiency for SDME-AgNPs was observed with the optimized parameters: extraction time 2 min, sample agitation rate 240 rpm, and sample pH 7. The limits of detection (LODs) of the SDME-AgNPs/AP-MALDI-MS technique for Met-enk and Leu-enk peptides were 160 and 210 nM, respectively. Furthermore, the application of the technique has been shown for the analysis of peptides from a sample containing high matrix interferences such as 1% Triton X-100 and 6 M urea. Finally, this approach has been compared with the SDME-AuNPs technique and the results have clearly revealed that the SDME-AgNP affinity probe exhibits higher affinity to extract the sulfur-bearing peptide (Met-enk). We also compared this electrostatic affinity probe of AgNPs with the previously demonstrated hydrophobic affinity probe of AgNPs and found that the electrostatic probe can greatly reduce the extraction time from 1.5 h to 2 min. This is due to the fact that electrostatic attraction forces are much stronger than the hydrophobic attraction forces. Therefore, we concluded that the electrostatic affinity probe based on SDME-AgNPs coupled with AP-MALDI-MS is a high-throughput technique for the analysis of low-abundance peptides from biological samples containing complex matrices. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18777509/Single_drop_microextraction_using_silver_nanoparticles_as_electrostatic_probes_for_peptide_analysis_in_atmospheric_pressure_matrix_assisted_laser_desorption/ionization_mass_spectrometry_and_comparison_with_gold_electrostatic_probes_and_silver_hydrophobic_probes_ L2 - https://doi.org/10.1002/rcm.3710 DB - PRIME DP - Unbound Medicine ER -