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Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells.
Immunology. 2009 May; 127(1):50-61.I

Abstract

Dendritic cells (DCs) are the most important antigen-presenting cells of the immune system and have a crucial role in T-lymphocyte activation and adaptive immunity initiation. However, DCs have also been implicated in maintaining immunological tolerance. In this study, we evaluated changes in the CD4(+) CD25(+) Foxp3(+) T-cell population after co-culture of lymph node cells from BALB/c mice with syngeneic bone marrow-derived DCs. Our results showed an increase in CD4(+) CD25(+) Foxp3(+) T cells after co-culture which occurred regardless of the activation state of DCs and the presence of allogeneic apoptotic cells; however, it was greater when DCs were immature and were pulsed with the alloantigen. Interestingly, syngeneic apoptotic thymocytes were not as efficient as allogeneic apoptotic cells in expanding the CD4(+) CD25(+) Foxp3(+) T-cell population. In all experimental settings, DCs produced high amounts of transforming growth factor (TGF)-beta. The presence of allogeneic apoptotic cells induced interleukin (IL)-2 production in immature and mature DC cultures. This cytokine was also detected in the supernatants under all experimental conditions and enhanced when immature DCs were pulsed with the alloantigen. CD4(+) CD25(+) Foxp3(+) T-cell expansion during co-culture of lymph node cells with DCs strongly suggested that the presence of alloantigen enhanced the number of regulatory T cells (Tregs) in vitro. Our data also suggest a role for both TGF-beta and IL-2 in the augmentation of the CD4(+) CD25(+) Foxp3(+) population.

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Authors+Show Affiliations

Marguti I
Laboratory of Clinical Immunology, Department of Immunology, Instituto de Ciências Biomédicas, University of São Paulo, São Paulo, Brazil.
Yamamoto GL
No affiliation info available
da Costa TB
No affiliation info available
Rizzo LV
No affiliation info available
de Moraes LV
No affiliation info available

MeSH

AnimalsApoptosisCell DifferentiationCell ProliferationCells, CulturedCoculture TechniquesDendritic CellsFemaleForkhead Transcription FactorsImmunophenotypingInterleukin-2Lymph NodesLymphocyte ActivationMiceMice, Inbred BALB CMice, Inbred C57BLPhagocytosisT-Lymphocytes, Regulatory

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18778287

Citation

Marguti, Ivo, et al. "Expansion of CD4+ CD25+ Foxp3+ T Cells By Bone Marrow-derived Dendritic Cells." Immunology, vol. 127, no. 1, 2009, pp. 50-61.
Marguti I, Yamamoto GL, da Costa TB, et al. Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells. Immunology. 2009;127(1):50-61.
Marguti, I., Yamamoto, G. L., da Costa, T. B., Rizzo, L. V., & de Moraes, L. V. (2009). Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells. Immunology, 127(1), 50-61. https://doi.org/10.1111/j.1365-2567.2008.02927.x
Marguti I, et al. Expansion of CD4+ CD25+ Foxp3+ T Cells By Bone Marrow-derived Dendritic Cells. Immunology. 2009;127(1):50-61. PubMed PMID: 18778287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells. AU - Marguti,Ivo, AU - Yamamoto,Guilherme Lopes, AU - da Costa,Thaís Boccia, AU - Rizzo,Luiz Vicente, AU - de Moraes,Luciana Vieira, PY - 2008/9/10/pubmed PY - 2009/6/18/medline PY - 2008/9/10/entrez SP - 50 EP - 61 JF - Immunology JO - Immunology VL - 127 IS - 1 N2 - Dendritic cells (DCs) are the most important antigen-presenting cells of the immune system and have a crucial role in T-lymphocyte activation and adaptive immunity initiation. However, DCs have also been implicated in maintaining immunological tolerance. In this study, we evaluated changes in the CD4(+) CD25(+) Foxp3(+) T-cell population after co-culture of lymph node cells from BALB/c mice with syngeneic bone marrow-derived DCs. Our results showed an increase in CD4(+) CD25(+) Foxp3(+) T cells after co-culture which occurred regardless of the activation state of DCs and the presence of allogeneic apoptotic cells; however, it was greater when DCs were immature and were pulsed with the alloantigen. Interestingly, syngeneic apoptotic thymocytes were not as efficient as allogeneic apoptotic cells in expanding the CD4(+) CD25(+) Foxp3(+) T-cell population. In all experimental settings, DCs produced high amounts of transforming growth factor (TGF)-beta. The presence of allogeneic apoptotic cells induced interleukin (IL)-2 production in immature and mature DC cultures. This cytokine was also detected in the supernatants under all experimental conditions and enhanced when immature DCs were pulsed with the alloantigen. CD4(+) CD25(+) Foxp3(+) T-cell expansion during co-culture of lymph node cells with DCs strongly suggested that the presence of alloantigen enhanced the number of regulatory T cells (Tregs) in vitro. Our data also suggest a role for both TGF-beta and IL-2 in the augmentation of the CD4(+) CD25(+) Foxp3(+) population. SN - 1365-2567 UR - https://www.unboundmedicine.com/medline/citation/18778287/Expansion_of_CD4+_CD25+_Foxp3+_T_cells_by_bone_marrow_derived_dendritic_cells_ L2 - https://doi.org/10.1111/j.1365-2567.2008.02927.x DB - PRIME DP - Unbound Medicine ER -