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Simultaneous quantitation of aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine in human plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetics evaluation of "SHEN-FU" injectable powder.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 01; 873(2):173-9.JC

Abstract

A rapid, specific and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was developed for simultaneous quantitation of six Aconitum alkaloids, i.e. aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA) and benzoylhypaconine (BHA) in human plasma collected from 18 healthy volunteers after intravenous drop infusion of "SHEN-FU" injectable powder in three different dosages. Lappaconitine was selected as the internal standard (IS). LC/MS/MS system coupled with an electrospray ionization (ESI) source was performed in multiple-reaction monitoring (MRM) mode. The transitions of the Aconitum alkaloids executed as following: m/z 646.3-->586.0 for AC; m/z 632.4-->573.1 for MA; m/z 616.2-->556.1 for HA; m/z 604.2-->104.8 for BAC; m/z 590.1-->104.8 for BMA; m/z 574.1-->104.8 for BHA; m/z 585.2-->161.8 for IS. Sample preparation was performed with solid-phase extraction (SPE) on a 1 mL HLB cartridge prior to analysis. The separation was applied on a Waters C(18) column (1.7 microm, 2.1 mm x 100 mm) and a gradient elution of methanol and 0.1% formic acid-water was used as mobile phase. The retention time was less than 4.5 min. The concentrations ranged from 0.1 to 1000 ng/mL for all six Aconitum alkaloids and showed a good linearity with the correlation coefficient (r(2)) >0.995. The validated method was employed to simultaneous quantitation and successfully used for the first time for the pharmacokinetic evaluation of the six Aconitum alkaloids after intravenous drop administration of "SHEN-FU" injectable powder in phase I clinical trial.

Authors+Show Affiliations

State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Keyuan Road 4, Gaopeng Street, Chengdu 610041, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18778973

Citation

Zhang, Fan, et al. "Simultaneous Quantitation of Aconitine, Mesaconitine, Hypaconitine, Benzoylaconine, Benzoylmesaconine and Benzoylhypaconine in Human Plasma By Liquid Chromatography-tandem Mass Spectrometry and Pharmacokinetics Evaluation of "SHEN-FU" Injectable Powder." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 873, no. 2, 2008, pp. 173-9.
Zhang F, Tang MH, Chen LJ, et al. Simultaneous quantitation of aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine in human plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetics evaluation of "SHEN-FU" injectable powder. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;873(2):173-9.
Zhang, F., Tang, M. H., Chen, L. J., Li, R., Wang, X. H., Duan, J. G., Zhao, X., & Wei, Y. Q. (2008). Simultaneous quantitation of aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine in human plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetics evaluation of "SHEN-FU" injectable powder. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 873(2), 173-9. https://doi.org/10.1016/j.jchromb.2008.08.008
Zhang F, et al. Simultaneous Quantitation of Aconitine, Mesaconitine, Hypaconitine, Benzoylaconine, Benzoylmesaconine and Benzoylhypaconine in Human Plasma By Liquid Chromatography-tandem Mass Spectrometry and Pharmacokinetics Evaluation of "SHEN-FU" Injectable Powder. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 1;873(2):173-9. PubMed PMID: 18778973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantitation of aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine in human plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetics evaluation of "SHEN-FU" injectable powder. AU - Zhang,Fan, AU - Tang,Ming-Hai, AU - Chen,Li-Juan, AU - Li,Rui, AU - Wang,Xian-Huo, AU - Duan,Jun-Guo, AU - Zhao,Xia, AU - Wei,Yu-Quan, Y1 - 2008/08/19/ PY - 2008/01/25/received PY - 2008/05/06/revised PY - 2008/08/13/accepted PY - 2008/9/10/pubmed PY - 2009/5/9/medline PY - 2008/9/10/entrez SP - 173 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 873 IS - 2 N2 - A rapid, specific and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was developed for simultaneous quantitation of six Aconitum alkaloids, i.e. aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA) and benzoylhypaconine (BHA) in human plasma collected from 18 healthy volunteers after intravenous drop infusion of "SHEN-FU" injectable powder in three different dosages. Lappaconitine was selected as the internal standard (IS). LC/MS/MS system coupled with an electrospray ionization (ESI) source was performed in multiple-reaction monitoring (MRM) mode. The transitions of the Aconitum alkaloids executed as following: m/z 646.3-->586.0 for AC; m/z 632.4-->573.1 for MA; m/z 616.2-->556.1 for HA; m/z 604.2-->104.8 for BAC; m/z 590.1-->104.8 for BMA; m/z 574.1-->104.8 for BHA; m/z 585.2-->161.8 for IS. Sample preparation was performed with solid-phase extraction (SPE) on a 1 mL HLB cartridge prior to analysis. The separation was applied on a Waters C(18) column (1.7 microm, 2.1 mm x 100 mm) and a gradient elution of methanol and 0.1% formic acid-water was used as mobile phase. The retention time was less than 4.5 min. The concentrations ranged from 0.1 to 1000 ng/mL for all six Aconitum alkaloids and showed a good linearity with the correlation coefficient (r(2)) >0.995. The validated method was employed to simultaneous quantitation and successfully used for the first time for the pharmacokinetic evaluation of the six Aconitum alkaloids after intravenous drop administration of "SHEN-FU" injectable powder in phase I clinical trial. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18778973/Simultaneous_quantitation_of_aconitine_mesaconitine_hypaconitine_benzoylaconine_benzoylmesaconine_and_benzoylhypaconine_in_human_plasma_by_liquid_chromatography_tandem_mass_spectrometry_and_pharmacokinetics_evaluation_of_"SHEN_FU"_injectable_powder_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00608-9 DB - PRIME DP - Unbound Medicine ER -