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Decrease in the synthesis and release of calcitonin gene-related peptide in dorsal root ganglia of spontaneously hypertensive rat: role of nitric oxide synthase inhibitors.
Eur J Pharmacol. 2008 Oct 31; 596(1-3):132-7.EJ

Abstract

Calcitonin gene-related peptide (CGRP), the major transmitter in capsaicin-sensitive sensory nerves, and asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, participate in the regulation of blood pressure. In the present study, we tested the relationship between CGRP and ADMA in spontaneously hypertensive rats (SHRs). For the in vivo study, SHRs were treated with or without L-arginine for 2 weeks, and Wistar Kyoto (WKY) rats were used as controls. Systolic arterial pressure was measured, and the levels of CGRP, ADMA, and NO were analyzed. For the in vitro study, neural cells from dorsal root ganglia were treated with NO inhibitor or donor. Synthesis and release of CGRP were measured. Compared with WKY rats, serum concentration of ADMA in SHRs increased while CGRP and NO level decreased. Treatment with L-arginine significantly decreased blood pressure, concomitantly with an increase in the level of NO and the synthesis and release of CGRP in SHRs, but it did not affect ADMA levels. In cultured neural cells, ADMA reduced the level of NO and inhibited the synthesis and release of CGRP in a concentration-dependent manner. The effects of ADMA were reversed by L-arginine. Treatment with NOC-18, a donor of NO, increased the release and synthesis of CGRP in neural cells in a concentration-dependent manner. Decreased synthesis and release of CGRP is related to a reduction in NO levels, and corresponds to the increased concentrations of ADMA in spontaneously hypertensive rats.

Authors+Show Affiliations

Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18782573

Citation

Chen, Qing-Quan, et al. "Decrease in the Synthesis and Release of Calcitonin Gene-related Peptide in Dorsal Root Ganglia of Spontaneously Hypertensive Rat: Role of Nitric Oxide Synthase Inhibitors." European Journal of Pharmacology, vol. 596, no. 1-3, 2008, pp. 132-7.
Chen QQ, Li D, Guo R, et al. Decrease in the synthesis and release of calcitonin gene-related peptide in dorsal root ganglia of spontaneously hypertensive rat: role of nitric oxide synthase inhibitors. Eur J Pharmacol. 2008;596(1-3):132-7.
Chen, Q. Q., Li, D., Guo, R., Luo, D., Yang, J., Hu, C. P., & Li, Y. J. (2008). Decrease in the synthesis and release of calcitonin gene-related peptide in dorsal root ganglia of spontaneously hypertensive rat: role of nitric oxide synthase inhibitors. European Journal of Pharmacology, 596(1-3), 132-7. https://doi.org/10.1016/j.ejphar.2008.08.029
Chen QQ, et al. Decrease in the Synthesis and Release of Calcitonin Gene-related Peptide in Dorsal Root Ganglia of Spontaneously Hypertensive Rat: Role of Nitric Oxide Synthase Inhibitors. Eur J Pharmacol. 2008 Oct 31;596(1-3):132-7. PubMed PMID: 18782573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decrease in the synthesis and release of calcitonin gene-related peptide in dorsal root ganglia of spontaneously hypertensive rat: role of nitric oxide synthase inhibitors. AU - Chen,Qing-Quan, AU - Li,Dai, AU - Guo,Ren, AU - Luo,Dan, AU - Yang,Jing, AU - Hu,Chang-Ping, AU - Li,Yuan-Jian, Y1 - 2008/08/30/ PY - 2008/04/27/received PY - 2008/08/04/revised PY - 2008/08/21/accepted PY - 2008/9/11/pubmed PY - 2009/1/13/medline PY - 2008/9/11/entrez SP - 132 EP - 7 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 596 IS - 1-3 N2 - Calcitonin gene-related peptide (CGRP), the major transmitter in capsaicin-sensitive sensory nerves, and asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, participate in the regulation of blood pressure. In the present study, we tested the relationship between CGRP and ADMA in spontaneously hypertensive rats (SHRs). For the in vivo study, SHRs were treated with or without L-arginine for 2 weeks, and Wistar Kyoto (WKY) rats were used as controls. Systolic arterial pressure was measured, and the levels of CGRP, ADMA, and NO were analyzed. For the in vitro study, neural cells from dorsal root ganglia were treated with NO inhibitor or donor. Synthesis and release of CGRP were measured. Compared with WKY rats, serum concentration of ADMA in SHRs increased while CGRP and NO level decreased. Treatment with L-arginine significantly decreased blood pressure, concomitantly with an increase in the level of NO and the synthesis and release of CGRP in SHRs, but it did not affect ADMA levels. In cultured neural cells, ADMA reduced the level of NO and inhibited the synthesis and release of CGRP in a concentration-dependent manner. The effects of ADMA were reversed by L-arginine. Treatment with NOC-18, a donor of NO, increased the release and synthesis of CGRP in neural cells in a concentration-dependent manner. Decreased synthesis and release of CGRP is related to a reduction in NO levels, and corresponds to the increased concentrations of ADMA in spontaneously hypertensive rats. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18782573/Decrease_in_the_synthesis_and_release_of_calcitonin_gene_related_peptide_in_dorsal_root_ganglia_of_spontaneously_hypertensive_rat:_role_of_nitric_oxide_synthase_inhibitors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(08)00906-0 DB - PRIME DP - Unbound Medicine ER -