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Selective and rapid immunomagnetic bead-based sample treatment for the liquid chromatography-electrospray ion-trap mass spectrometry detection of Ara h3/4 peanut protein in foods.
J Chromatogr A. 2008 Oct 10; 1206(2):89-94.JC

Abstract

Complex matrices commonly affect the sensitivity and selectivity of liquid chromatography-mass spectrometry (LC-MS) analysis. Thus, selective sample enrichment strategies are useful particularly to analyze organic biomarkers present in low abundance in samples. A selective immunomagnetic extraction procedure to isolate trace peanut allergen protein Ara h3/4 from breakfast cereals combined with microwave-assisted tryptic digestion and liquid chromatography-electrospray ion-trap tandem mass spectrometry (LC-ESI-IT-MS/MS) measurement was developed. Using protein A-coated magnetic bead (MB) support, anti-Ara h3/4 monoclonal antibodies (Abs) were used as selective capture molecules. The results obtained by LC-ESI-IT-MS/MS in terms of limit of detection (3 mg peanuts/kg matrix) and a significantly reduced matrix effect demonstrated that the Ab-coated magnetic bead was very effective to selectively trap Ara h3/4 protein in breakfast cereals. The magnetic bead-based sample treatment followed by LC-IT-MS/MS method here developed can be proposed as very rapid and powerful confirmatory analytical method to verify the reliability of enzyme-linked immunosorbent assay (ELISA) screening methods, since the magnetic bead-LC-IT-MS/MS method combines good sensitivity to the identification capabilities of mass spectrometry.

Authors+Show Affiliations

Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Viale G.P. Usberti 17/A, I-43100 Parma, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18783783

Citation

Careri, Maria, et al. "Selective and Rapid Immunomagnetic Bead-based Sample Treatment for the Liquid Chromatography-electrospray Ion-trap Mass Spectrometry Detection of Ara H3/4 Peanut Protein in Foods." Journal of Chromatography. A, vol. 1206, no. 2, 2008, pp. 89-94.
Careri M, Elviri L, Lagos JB, et al. Selective and rapid immunomagnetic bead-based sample treatment for the liquid chromatography-electrospray ion-trap mass spectrometry detection of Ara h3/4 peanut protein in foods. J Chromatogr A. 2008;1206(2):89-94.
Careri, M., Elviri, L., Lagos, J. B., Mangia, A., Speroni, F., & Terenghi, M. (2008). Selective and rapid immunomagnetic bead-based sample treatment for the liquid chromatography-electrospray ion-trap mass spectrometry detection of Ara h3/4 peanut protein in foods. Journal of Chromatography. A, 1206(2), 89-94. https://doi.org/10.1016/j.chroma.2008.07.091
Careri M, et al. Selective and Rapid Immunomagnetic Bead-based Sample Treatment for the Liquid Chromatography-electrospray Ion-trap Mass Spectrometry Detection of Ara H3/4 Peanut Protein in Foods. J Chromatogr A. 2008 Oct 10;1206(2):89-94. PubMed PMID: 18783783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective and rapid immunomagnetic bead-based sample treatment for the liquid chromatography-electrospray ion-trap mass spectrometry detection of Ara h3/4 peanut protein in foods. AU - Careri,Maria, AU - Elviri,Lisa, AU - Lagos,Jesse Boquett, AU - Mangia,Alessandro, AU - Speroni,Francesca, AU - Terenghi,Mattia, Y1 - 2008/08/07/ PY - 2008/04/02/received PY - 2008/07/23/revised PY - 2008/07/24/accepted PY - 2008/9/12/pubmed PY - 2008/12/17/medline PY - 2008/9/12/entrez SP - 89 EP - 94 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1206 IS - 2 N2 - Complex matrices commonly affect the sensitivity and selectivity of liquid chromatography-mass spectrometry (LC-MS) analysis. Thus, selective sample enrichment strategies are useful particularly to analyze organic biomarkers present in low abundance in samples. A selective immunomagnetic extraction procedure to isolate trace peanut allergen protein Ara h3/4 from breakfast cereals combined with microwave-assisted tryptic digestion and liquid chromatography-electrospray ion-trap tandem mass spectrometry (LC-ESI-IT-MS/MS) measurement was developed. Using protein A-coated magnetic bead (MB) support, anti-Ara h3/4 monoclonal antibodies (Abs) were used as selective capture molecules. The results obtained by LC-ESI-IT-MS/MS in terms of limit of detection (3 mg peanuts/kg matrix) and a significantly reduced matrix effect demonstrated that the Ab-coated magnetic bead was very effective to selectively trap Ara h3/4 protein in breakfast cereals. The magnetic bead-based sample treatment followed by LC-IT-MS/MS method here developed can be proposed as very rapid and powerful confirmatory analytical method to verify the reliability of enzyme-linked immunosorbent assay (ELISA) screening methods, since the magnetic bead-LC-IT-MS/MS method combines good sensitivity to the identification capabilities of mass spectrometry. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18783783/Selective_and_rapid_immunomagnetic_bead_based_sample_treatment_for_the_liquid_chromatography_electrospray_ion_trap_mass_spectrometry_detection_of_Ara_h3/4_peanut_protein_in_foods_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01270-3 DB - PRIME DP - Unbound Medicine ER -