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Intrarenal RAS activity and urinary angiotensinogen excretion in anti-thymocyte serum nephritis rats.
Am J Physiol Renal Physiol. 2008 Nov; 295(5):F1512-8.AJ

Abstract

The differential roles of circulating and intrarenal renin-angiotensin system (RAS) in glomerulonephritis have not been elucidated. In this study, we investigated the levels of circulating and intrarenal RAS activity and urinary angiotensinogen (AGT) excretion in anti-thymocyte serum (ATS) nephritis induced by an ATS injection (ATS group). The effect of olmesartan, an angiotensin II (ANG II) type 1 receptor blocker (ARB), on the development of nephritis was also examined (ATS+ARB group). In addition, the rats received a saline injection instead of ATS (control group). Mesangial proliferation with transient proteinuria, which peaked at day 7, was significantly increased in the ATS group compared with the control group. The levels of glomerular AGT mRNA, intrarenal ANG II, and urinary AGT excretion in the ATS group were increased significantly at day 7 compared with the control group. Administration of olmesartan (ATS+ARB group) significantly decreased the levels of renal lesions, proteinuria, and intrarenal RAS activity compared with the ATS group. In addition, the levels of urinary AGT excretion correlated with the levels of glomerular damage, urinary protein excretion, and immunoreactivity for AGT and ANG II in kidney. On the other hand, plasma renin activity was significantly lower in the ATS group compared with the control group and significantly higher in the ATS+ARB group than in the ATS group. These data suggest that an increase in kidney-specific RAS activity, which parallels urinary AGT excretion, plays an important role in the development of ATS nephritis.

Authors+Show Affiliations

First Department of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan. nohashi@tulane.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18784264

Citation

Ohashi, Naro, et al. "Intrarenal RAS Activity and Urinary Angiotensinogen Excretion in Anti-thymocyte Serum Nephritis Rats." American Journal of Physiology. Renal Physiology, vol. 295, no. 5, 2008, pp. F1512-8.
Ohashi N, Yamamoto T, Huang Y, et al. Intrarenal RAS activity and urinary angiotensinogen excretion in anti-thymocyte serum nephritis rats. Am J Physiol Renal Physiol. 2008;295(5):F1512-8.
Ohashi, N., Yamamoto, T., Huang, Y., Misaki, T., Fukasawa, H., Suzuki, H., Togawa, A., Suzuki, S., Fujigaki, Y., Nakagawa, T., Nakamura, Y., Suzuki, F., Kitagawa, M., & Hishida, A. (2008). Intrarenal RAS activity and urinary angiotensinogen excretion in anti-thymocyte serum nephritis rats. American Journal of Physiology. Renal Physiology, 295(5), F1512-8. https://doi.org/10.1152/ajprenal.00058.2008
Ohashi N, et al. Intrarenal RAS Activity and Urinary Angiotensinogen Excretion in Anti-thymocyte Serum Nephritis Rats. Am J Physiol Renal Physiol. 2008;295(5):F1512-8. PubMed PMID: 18784264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intrarenal RAS activity and urinary angiotensinogen excretion in anti-thymocyte serum nephritis rats. AU - Ohashi,Naro, AU - Yamamoto,Tatsuo, AU - Huang,Yanjie, AU - Misaki,Taro, AU - Fukasawa,Hirotaka, AU - Suzuki,Hiroyuki, AU - Togawa,Akashi, AU - Suzuki,Sayuri, AU - Fujigaki,Yoshihide, AU - Nakagawa,Tsutomu, AU - Nakamura,Yukio, AU - Suzuki,Fumiaki, AU - Kitagawa,Masatoshi, AU - Hishida,Akira, Y1 - 2008/09/10/ PY - 2008/9/12/pubmed PY - 2009/2/10/medline PY - 2008/9/12/entrez SP - F1512 EP - 8 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 295 IS - 5 N2 - The differential roles of circulating and intrarenal renin-angiotensin system (RAS) in glomerulonephritis have not been elucidated. In this study, we investigated the levels of circulating and intrarenal RAS activity and urinary angiotensinogen (AGT) excretion in anti-thymocyte serum (ATS) nephritis induced by an ATS injection (ATS group). The effect of olmesartan, an angiotensin II (ANG II) type 1 receptor blocker (ARB), on the development of nephritis was also examined (ATS+ARB group). In addition, the rats received a saline injection instead of ATS (control group). Mesangial proliferation with transient proteinuria, which peaked at day 7, was significantly increased in the ATS group compared with the control group. The levels of glomerular AGT mRNA, intrarenal ANG II, and urinary AGT excretion in the ATS group were increased significantly at day 7 compared with the control group. Administration of olmesartan (ATS+ARB group) significantly decreased the levels of renal lesions, proteinuria, and intrarenal RAS activity compared with the ATS group. In addition, the levels of urinary AGT excretion correlated with the levels of glomerular damage, urinary protein excretion, and immunoreactivity for AGT and ANG II in kidney. On the other hand, plasma renin activity was significantly lower in the ATS group compared with the control group and significantly higher in the ATS+ARB group than in the ATS group. These data suggest that an increase in kidney-specific RAS activity, which parallels urinary AGT excretion, plays an important role in the development of ATS nephritis. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/18784264/Intrarenal_RAS_activity_and_urinary_angiotensinogen_excretion_in_anti_thymocyte_serum_nephritis_rats_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.00058.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -