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Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: role of altered gene expression.
Lipids 2008; 43(11):999-1008L

Abstract

In vitro cultivated Atlantic salmon (Salmo salar L.), hepatocytes were incubated without or with a mixture of sesamin and episesamin in order to test for possible effects on lipid metabolism. Sesamin/episesamin exposure (0.05 mM, final concentration) led to increased elongation and desaturation of (14)C 18:3n-3 to docosahexaenoic acid ((14)C 22:6n-3, DHA, P < 0.01) and down regulated gene expression of Delta6 and Delta5 desaturases compared to control treatment. Sesamin/episesamin further increased the hepatocytes capacity for fatty acid beta-oxidation of (14)C 18:3n-3 (P < 0.01) to the (14)C acid soluble products, acetate, malate and oxaloacetate, in agreement with an increased gene expression of carnitine palmitoyltransferase I. Also the gene expression of cluster of differentiation 36 was upregulated and the expression of scavenger receptor type B, peroxisome proliferator-activated receptors alpha and gamma were downregulated. The amount of triacylglycerols secreted by the cells tended to be lower in the sesamin/episesamin incubated hepatocytes than the control cells. This study shows that sesamin has favourable effects on lipid metabolism leading to increased level of DHA, which may be of interest for aquaculture use.

Authors+Show Affiliations

Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, 75007, Uppsala, Sweden. Sofia.Trattner@lmv.slu.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18784949

Citation

Trattner, Sofia, et al. "Sesamin Increases Alpha-linolenic Acid Conversion to Docosahexaenoic Acid in Atlantic Salmon (Salmo Salar L.) Hepatocytes: Role of Altered Gene Expression." Lipids, vol. 43, no. 11, 2008, pp. 999-1008.
Trattner S, Ruyter B, Østbye TK, et al. Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: role of altered gene expression. Lipids. 2008;43(11):999-1008.
Trattner, S., Ruyter, B., Østbye, T. K., Gjøen, T., Zlabek, V., Kamal-Eldin, A., & Pickova, J. (2008). Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: role of altered gene expression. Lipids, 43(11), pp. 999-1008. doi:10.1007/s11745-008-3229-7.
Trattner S, et al. Sesamin Increases Alpha-linolenic Acid Conversion to Docosahexaenoic Acid in Atlantic Salmon (Salmo Salar L.) Hepatocytes: Role of Altered Gene Expression. Lipids. 2008;43(11):999-1008. PubMed PMID: 18784949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: role of altered gene expression. AU - Trattner,Sofia, AU - Ruyter,B, AU - Østbye,T K, AU - Gjøen,T, AU - Zlabek,V, AU - Kamal-Eldin,A, AU - Pickova,J, Y1 - 2008/09/11/ PY - 2008/06/15/received PY - 2008/08/14/accepted PY - 2008/9/12/pubmed PY - 2009/3/19/medline PY - 2008/9/12/entrez SP - 999 EP - 1008 JF - Lipids JO - Lipids VL - 43 IS - 11 N2 - In vitro cultivated Atlantic salmon (Salmo salar L.), hepatocytes were incubated without or with a mixture of sesamin and episesamin in order to test for possible effects on lipid metabolism. Sesamin/episesamin exposure (0.05 mM, final concentration) led to increased elongation and desaturation of (14)C 18:3n-3 to docosahexaenoic acid ((14)C 22:6n-3, DHA, P < 0.01) and down regulated gene expression of Delta6 and Delta5 desaturases compared to control treatment. Sesamin/episesamin further increased the hepatocytes capacity for fatty acid beta-oxidation of (14)C 18:3n-3 (P < 0.01) to the (14)C acid soluble products, acetate, malate and oxaloacetate, in agreement with an increased gene expression of carnitine palmitoyltransferase I. Also the gene expression of cluster of differentiation 36 was upregulated and the expression of scavenger receptor type B, peroxisome proliferator-activated receptors alpha and gamma were downregulated. The amount of triacylglycerols secreted by the cells tended to be lower in the sesamin/episesamin incubated hepatocytes than the control cells. This study shows that sesamin has favourable effects on lipid metabolism leading to increased level of DHA, which may be of interest for aquaculture use. SN - 0024-4201 UR - https://www.unboundmedicine.com/medline/citation/18784949/Sesamin_increases_alpha_linolenic_acid_conversion_to_docosahexaenoic_acid_in_atlantic_salmon__Salmo_salar_L___hepatocytes:_role_of_altered_gene_expression_ L2 - https://link.springer.com/article/10.1007/s11745-008-3229-7 DB - PRIME DP - Unbound Medicine ER -