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Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser-induced fluorescence detection.
J Sep Sci. 2008 Sep; 31(16-17):3088-97.JS

Abstract

A CE-LIF detection method has been developed to identify and quantitate six amino acid neurotransmitters including glutamic acid, aspartic acid, gamma-aminobutyric acid, glycine, taurine, and glutamine. N-hydroxysuccinimidyl fluorescein-O-acetate, a fluorescein-based dye, was employed for the derivatization of these neurotransmitters prior to CE-LIF analysis. Different parameters which influenced separation and derivatization were optimized in detail. Under optimum conditions, linearity was achieved within concentration ranges of up to three orders of magnitudes for those analytes with correlation coefficients from 0.9989 to 0.9998. The LODs ranged from 0.06 nM to 0.1 nM, and are thus superior or equivalent to those previously reported in the literature using CE-LIF detection. The proposed method has been successfully applied to the determination of amino acid neurotransmitters in biological samples such as human cerebrospinal fluid and saliva with satisfactory recoveries.

Authors+Show Affiliations

Department of Chemistry, Wuhan University, Wuhan, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18785139

Citation

Deng, Ying-Hua, et al. "Determination of Amino Acid Neurotransmitters in Human Cerebrospinal Fluid and Saliva By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Separation Science, vol. 31, no. 16-17, 2008, pp. 3088-97.
Deng YH, Wang H, Zhang HS. Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser-induced fluorescence detection. J Sep Sci. 2008;31(16-17):3088-97.
Deng, Y. H., Wang, H., & Zhang, H. S. (2008). Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser-induced fluorescence detection. Journal of Separation Science, 31(16-17), 3088-97. https://doi.org/10.1002/jssc.200800339
Deng YH, Wang H, Zhang HS. Determination of Amino Acid Neurotransmitters in Human Cerebrospinal Fluid and Saliva By Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Sep Sci. 2008;31(16-17):3088-97. PubMed PMID: 18785139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser-induced fluorescence detection. AU - Deng,Ying-Hua, AU - Wang,Hong, AU - Zhang,Hua-Shan, PY - 2008/9/12/pubmed PY - 2008/12/20/medline PY - 2008/9/12/entrez SP - 3088 EP - 97 JF - Journal of separation science JO - J Sep Sci VL - 31 IS - 16-17 N2 - A CE-LIF detection method has been developed to identify and quantitate six amino acid neurotransmitters including glutamic acid, aspartic acid, gamma-aminobutyric acid, glycine, taurine, and glutamine. N-hydroxysuccinimidyl fluorescein-O-acetate, a fluorescein-based dye, was employed for the derivatization of these neurotransmitters prior to CE-LIF analysis. Different parameters which influenced separation and derivatization were optimized in detail. Under optimum conditions, linearity was achieved within concentration ranges of up to three orders of magnitudes for those analytes with correlation coefficients from 0.9989 to 0.9998. The LODs ranged from 0.06 nM to 0.1 nM, and are thus superior or equivalent to those previously reported in the literature using CE-LIF detection. The proposed method has been successfully applied to the determination of amino acid neurotransmitters in biological samples such as human cerebrospinal fluid and saliva with satisfactory recoveries. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/18785139/Determination_of_amino_acid_neurotransmitters_in_human_cerebrospinal_fluid_and_saliva_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/jssc.200800339 DB - PRIME DP - Unbound Medicine ER -