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A perivascular origin for mesenchymal stem cells in multiple human organs.
Cell Stem Cell 2008; 3(3):301-13CS

Abstract

Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rbeta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.

Authors+Show Affiliations

Department of Pediatrics, Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18786417

Citation

Crisan, Mihaela, et al. "A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human Organs." Cell Stem Cell, vol. 3, no. 3, 2008, pp. 301-13.
Crisan M, Yap S, Casteilla L, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008;3(3):301-13.
Crisan, M., Yap, S., Casteilla, L., Chen, C. W., Corselli, M., Park, T. S., ... Péault, B. (2008). A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell, 3(3), pp. 301-13. doi:10.1016/j.stem.2008.07.003.
Crisan M, et al. A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human Organs. Cell Stem Cell. 2008 Sep 11;3(3):301-13. PubMed PMID: 18786417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A perivascular origin for mesenchymal stem cells in multiple human organs. AU - Crisan,Mihaela, AU - Yap,Solomon, AU - Casteilla,Louis, AU - Chen,Chien-Wen, AU - Corselli,Mirko, AU - Park,Tea Soon, AU - Andriolo,Gabriella, AU - Sun,Bin, AU - Zheng,Bo, AU - Zhang,Li, AU - Norotte,Cyrille, AU - Teng,Pang-Ning, AU - Traas,Jeremy, AU - Schugar,Rebecca, AU - Deasy,Bridget M, AU - Badylak,Stephen, AU - Buhring,Hans-Jörg, AU - Giacobino,Jean-Paul, AU - Lazzari,Lorenza, AU - Huard,Johnny, AU - Péault,Bruno, PY - 2008/02/18/received PY - 2008/06/04/revised PY - 2008/07/07/accepted PY - 2008/9/13/pubmed PY - 2008/10/23/medline PY - 2008/9/13/entrez SP - 301 EP - 13 JF - Cell stem cell JO - Cell Stem Cell VL - 3 IS - 3 N2 - Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rbeta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells. SN - 1875-9777 UR - https://www.unboundmedicine.com/medline/citation/18786417/A_perivascular_origin_for_mesenchymal_stem_cells_in_multiple_human_organs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1934-5909(08)00337-8 DB - PRIME DP - Unbound Medicine ER -