Tags

Type your tag names separated by a space and hit enter

Resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress.
Eur J Pharmacol. 2008 Oct 31; 596(1-3):146-52.EJ

Abstract

Previously, we reported that pyrogallol, an anti-psoriatic agent, causes hepatotoxicity in experimental animals and silymarin, an herbal antioxidant, reduces pyrogallol-induced changes [Upadhyay, G., Kumar, A., Singh, M.P., 2007. Effect of silymarin on pyrogallol- and rifampicin-induced hepatotoxicity in mouse. Eur. J. Pharmacol. 565, 190-201.]. The present study was undertaken to assess the effect of resveratrol against pyrogallol-induced changes in hepatic damage markers, xenobiotic metabolizing enzymes and oxidative stress. Swiss albino mice were treated intraperitoneally, daily with pyrogallol (40 mg/kg), for one to four weeks, along with respective controls. In some set of experiments, animals were pre-treated with resveratrol (10 mg/kg), 2 h prior to pyrogallol treatment, along with respective controls. Alanine aminotransaminase, aspartate aminotransaminase and bilirubin were measured in blood plasma and mRNA expression of cytochrome P-450 (CYP) 1A1, CYP1A2, CYP2E1, glutathione-S-transferase (GST)-ya and GST-yc, catalytic activity of CYP1A1, CYP1A2, CYP2E1, GST, glutathione reductase and glutathione peroxidase, lipid peroxidation and reduced glutathione (GSH) level were measured in liver. Resveratrol reduced pyrogallol-mediated increase in alanine aminotransaminase, aspartate aminotransaminase, bilirubin, lipid peroxidation and mRNA expression and catalytic activity of CYP2E1 and CYP1A2. Pyrogallol-mediated decrease in GST-ya and GST-yc expressions, GST, glutathione peroxidase and glutathione reductase activities and GSH content was significantly attenuated in resveratrol co-treated animals. CYP1A1 expression and catalytic activity were not altered significantly in any treated groups. The results demonstrate that resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress.

Authors+Show Affiliations

Indian Institute of Toxicology Research (IITR), Lucknow, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18789925

Citation

Upadhyay, Ghanshyam, et al. "Resveratrol Modulates Pyrogallol-induced Changes in Hepatic Toxicity Markers, Xenobiotic Metabolizing Enzymes and Oxidative Stress." European Journal of Pharmacology, vol. 596, no. 1-3, 2008, pp. 146-52.
Upadhyay G, Singh AK, Kumar A, et al. Resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress. Eur J Pharmacol. 2008;596(1-3):146-52.
Upadhyay, G., Singh, A. K., Kumar, A., Prakash, O., & Singh, M. P. (2008). Resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress. European Journal of Pharmacology, 596(1-3), 146-52. https://doi.org/10.1016/j.ejphar.2008.08.019
Upadhyay G, et al. Resveratrol Modulates Pyrogallol-induced Changes in Hepatic Toxicity Markers, Xenobiotic Metabolizing Enzymes and Oxidative Stress. Eur J Pharmacol. 2008 Oct 31;596(1-3):146-52. PubMed PMID: 18789925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress. AU - Upadhyay,Ghanshyam, AU - Singh,Anand Kumar, AU - Kumar,Abhai, AU - Prakash,Om, AU - Singh,Mahendra Pratap, Y1 - 2008/08/30/ PY - 2008/07/07/received PY - 2008/08/05/revised PY - 2008/08/22/accepted PY - 2008/9/16/pubmed PY - 2009/1/13/medline PY - 2008/9/16/entrez SP - 146 EP - 52 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 596 IS - 1-3 N2 - Previously, we reported that pyrogallol, an anti-psoriatic agent, causes hepatotoxicity in experimental animals and silymarin, an herbal antioxidant, reduces pyrogallol-induced changes [Upadhyay, G., Kumar, A., Singh, M.P., 2007. Effect of silymarin on pyrogallol- and rifampicin-induced hepatotoxicity in mouse. Eur. J. Pharmacol. 565, 190-201.]. The present study was undertaken to assess the effect of resveratrol against pyrogallol-induced changes in hepatic damage markers, xenobiotic metabolizing enzymes and oxidative stress. Swiss albino mice were treated intraperitoneally, daily with pyrogallol (40 mg/kg), for one to four weeks, along with respective controls. In some set of experiments, animals were pre-treated with resveratrol (10 mg/kg), 2 h prior to pyrogallol treatment, along with respective controls. Alanine aminotransaminase, aspartate aminotransaminase and bilirubin were measured in blood plasma and mRNA expression of cytochrome P-450 (CYP) 1A1, CYP1A2, CYP2E1, glutathione-S-transferase (GST)-ya and GST-yc, catalytic activity of CYP1A1, CYP1A2, CYP2E1, GST, glutathione reductase and glutathione peroxidase, lipid peroxidation and reduced glutathione (GSH) level were measured in liver. Resveratrol reduced pyrogallol-mediated increase in alanine aminotransaminase, aspartate aminotransaminase, bilirubin, lipid peroxidation and mRNA expression and catalytic activity of CYP2E1 and CYP1A2. Pyrogallol-mediated decrease in GST-ya and GST-yc expressions, GST, glutathione peroxidase and glutathione reductase activities and GSH content was significantly attenuated in resveratrol co-treated animals. CYP1A1 expression and catalytic activity were not altered significantly in any treated groups. The results demonstrate that resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18789925/Resveratrol_modulates_pyrogallol_induced_changes_in_hepatic_toxicity_markers_xenobiotic_metabolizing_enzymes_and_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(08)00894-7 DB - PRIME DP - Unbound Medicine ER -