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Effects of tetrandrine on ischemia/reperfusion injury in mouse liver.
Transplant Proc. 2008 Sep; 40(7):2163-6.TP

Abstract

OBJECTIVE

Hepatic ischemia/reperfusion injury (IRI) may cause acute inflammatory damage, producing significant organ dysfunction, an important problem for liver transplantation. Previous studies have demonstrated that Tetrandrine (Tet), a component of traditional Chinese herbal medicine, shows protective effects to scavenge active oxygen radicals and inhibit lipid peroxidation. In this study, we examined whether Tet has a protective effect on mouse hepatic IRI.

MATERIALS AND METHODS

Male C57BL/6 mice were divided into sham, ischemic, and Tet-treated groups; 90 minutes of warm ischemia was performed on the left liver lobe. Tet (20 mg/kg) was injected intraperitoneally at 1 hour before ischemia with a second intravenous dose was injected just before reperfusion. Blood and liver samples were collected at 6 hours after reperfusion. We analyzed the hepatocellular injury, oxidative stress, neutrophil recruitment, and tumor necrosis factor-alpha (TNF-alpha) generation associated with hepatic IRI.

RESULTS

Undergoing 90 minutes of ischemia and 6 hours reperfusion caused dramatic injuries in mouse livers. Administration of Tet (20 mg/kg) reduced serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH); decreased liver edema, TNF-alpha, myeloperoxidase (MPO) and malondialdehyde (MDA) contents; and ameliorated the down-regulation of superoxide dismutase (SOD) activity.

CONCLUSION

Tet showed protective effects on mouse hepatic IRI.

Authors+Show Affiliations

Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18790181

Citation

Cheng, F, et al. "Effects of Tetrandrine On Ischemia/reperfusion Injury in Mouse Liver." Transplantation Proceedings, vol. 40, no. 7, 2008, pp. 2163-6.
Cheng F, Li Y, Feng L, et al. Effects of tetrandrine on ischemia/reperfusion injury in mouse liver. Transplant Proc. 2008;40(7):2163-6.
Cheng, F., Li, Y., Feng, L., & Li, S. (2008). Effects of tetrandrine on ischemia/reperfusion injury in mouse liver. Transplantation Proceedings, 40(7), 2163-6. https://doi.org/10.1016/j.transproceed.2008.07.082
Cheng F, et al. Effects of Tetrandrine On Ischemia/reperfusion Injury in Mouse Liver. Transplant Proc. 2008;40(7):2163-6. PubMed PMID: 18790181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of tetrandrine on ischemia/reperfusion injury in mouse liver. AU - Cheng,F, AU - Li,Y, AU - Feng,L, AU - Li,S, PY - 2008/9/16/pubmed PY - 2008/11/13/medline PY - 2008/9/16/entrez SP - 2163 EP - 6 JF - Transplantation proceedings JO - Transplant Proc VL - 40 IS - 7 N2 - OBJECTIVE: Hepatic ischemia/reperfusion injury (IRI) may cause acute inflammatory damage, producing significant organ dysfunction, an important problem for liver transplantation. Previous studies have demonstrated that Tetrandrine (Tet), a component of traditional Chinese herbal medicine, shows protective effects to scavenge active oxygen radicals and inhibit lipid peroxidation. In this study, we examined whether Tet has a protective effect on mouse hepatic IRI. MATERIALS AND METHODS: Male C57BL/6 mice were divided into sham, ischemic, and Tet-treated groups; 90 minutes of warm ischemia was performed on the left liver lobe. Tet (20 mg/kg) was injected intraperitoneally at 1 hour before ischemia with a second intravenous dose was injected just before reperfusion. Blood and liver samples were collected at 6 hours after reperfusion. We analyzed the hepatocellular injury, oxidative stress, neutrophil recruitment, and tumor necrosis factor-alpha (TNF-alpha) generation associated with hepatic IRI. RESULTS: Undergoing 90 minutes of ischemia and 6 hours reperfusion caused dramatic injuries in mouse livers. Administration of Tet (20 mg/kg) reduced serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH); decreased liver edema, TNF-alpha, myeloperoxidase (MPO) and malondialdehyde (MDA) contents; and ameliorated the down-regulation of superoxide dismutase (SOD) activity. CONCLUSION: Tet showed protective effects on mouse hepatic IRI. SN - 0041-1345 UR - https://www.unboundmedicine.com/medline/citation/18790181/Effects_of_tetrandrine_on_ischemia/reperfusion_injury_in_mouse_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(08)00919-6 DB - PRIME DP - Unbound Medicine ER -