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Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex.
Int J Antimicrob Agents. 2009 Jan; 33(1):27-32.IJ

Abstract

Of 112 non-repetitive clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex, 80% were resistant to a variety of structurally unrelated antimicrobials although all isolates were susceptible to minocycline and polymyxin. Resistance to carbapenems occurred in 8% of the isolates. The presence of adeSR-adeABC, adeDE and adeIJK drug efflux system genes and class 1 integron genes (integrase gene int1) was assessed by polymerase chain reaction (PCR) in relation to the susceptibility of the isolates to 20 antimicrobials. The majority of isolates (75%) with high levels of multidrug resistance were positive for adeSR-adeABC and adeIJK as well as int1 and thus belong to A. baumannii (i.e. genomospecies 2). Positive adeE was only observed in adeSR-adeABC/adeIJK/int1-negative isolates (8%; likely belonging to Acinetobacter genomospecies 3) that were relatively susceptible to several agents, and adeE expression was undetectable. The results reveal a possible association between adeABC/adeIJK and int1 in multidrug-resistant isolates of A. baumannii. In addition, differential distribution of the resistance-nodulation-cell division (RND) genes can likely be used as indicators for differentiating Acinetobacter species.

Authors+Show Affiliations

Institute of Materia Medica and Department of Pharmacology, North Sichuan Medical College, Nanchong, Sichuan 637007, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18790612

Citation

Lin, Li, et al. "Distribution of the Multidrug Efflux Pump Genes, adeABC, adeDE and adeIJK, and Class 1 Integron Genes in Multiple-antimicrobial-resistant Clinical Isolates of Acinetobacter baumannii-Acinetobacter Calcoaceticus Complex." International Journal of Antimicrobial Agents, vol. 33, no. 1, 2009, pp. 27-32.
Lin L, Ling BD, Li XZ. Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex. Int J Antimicrob Agents. 2009;33(1):27-32.
Lin, L., Ling, B. D., & Li, X. Z. (2009). Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex. International Journal of Antimicrobial Agents, 33(1), 27-32. https://doi.org/10.1016/j.ijantimicag.2008.06.027
Lin L, Ling BD, Li XZ. Distribution of the Multidrug Efflux Pump Genes, adeABC, adeDE and adeIJK, and Class 1 Integron Genes in Multiple-antimicrobial-resistant Clinical Isolates of Acinetobacter baumannii-Acinetobacter Calcoaceticus Complex. Int J Antimicrob Agents. 2009;33(1):27-32. PubMed PMID: 18790612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex. AU - Lin,Li, AU - Ling,Bao-Dong, AU - Li,Xian-Zhi, Y1 - 2008/09/13/ PY - 2008/04/21/received PY - 2008/06/12/revised PY - 2008/06/25/accepted PY - 2008/9/16/pubmed PY - 2009/2/25/medline PY - 2008/9/16/entrez SP - 27 EP - 32 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 33 IS - 1 N2 - Of 112 non-repetitive clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex, 80% were resistant to a variety of structurally unrelated antimicrobials although all isolates were susceptible to minocycline and polymyxin. Resistance to carbapenems occurred in 8% of the isolates. The presence of adeSR-adeABC, adeDE and adeIJK drug efflux system genes and class 1 integron genes (integrase gene int1) was assessed by polymerase chain reaction (PCR) in relation to the susceptibility of the isolates to 20 antimicrobials. The majority of isolates (75%) with high levels of multidrug resistance were positive for adeSR-adeABC and adeIJK as well as int1 and thus belong to A. baumannii (i.e. genomospecies 2). Positive adeE was only observed in adeSR-adeABC/adeIJK/int1-negative isolates (8%; likely belonging to Acinetobacter genomospecies 3) that were relatively susceptible to several agents, and adeE expression was undetectable. The results reveal a possible association between adeABC/adeIJK and int1 in multidrug-resistant isolates of A. baumannii. In addition, differential distribution of the resistance-nodulation-cell division (RND) genes can likely be used as indicators for differentiating Acinetobacter species. SN - 0924-8579 UR - https://www.unboundmedicine.com/medline/citation/18790612/Distribution_of_the_multidrug_efflux_pump_genes_adeABC_adeDE_and_adeIJK_and_class_1_integron_genes_in_multiple_antimicrobial_resistant_clinical_isolates_of_Acinetobacter_baumannii_Acinetobacter_calcoaceticus_complex_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(08)00303-8 DB - PRIME DP - Unbound Medicine ER -