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Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of Delta9-desaturase in muscle of beef cattle.
J Anim Sci. 2009 Jan; 87(1):244-52.JA

Abstract

The objective of this study was to examine the effect of level and duration of feeding of an n-3 PUFA-enriched fish oil (FO) supplement in combination with soybean oil (SO) on the transcriptional regulation of Delta(9)-desaturase gene expression in bovine muscle. Beef bulls (n = 40) were assigned to 1 of 4 iso-lipid and isonitrogenous concentrate diets fed for ad libitum intake for a 100-d finishing period. Concentrates were supplemented with one of the following: 1) 6% SO (CON); 2) 6% SO + 1% FO (FO1); 3) 6% SO + 2% FO (FO2); or 4) 8% palmitic acid for the first 50 d and 6% SO + 2% FO for the second 50 d [FO2(50)]. Samples of LM were harvested and concentrations of fatty acids were measured. Total RNA was isolated and the gene expression of Delta(9)-desaturase was determined. The mRNA expression of putative regulators of Delta(9)-desaturase gene expression, sterol regulatory element binding protein-1c (SREBP-1c) and peroxisome proliferator activated receptor-alpha (PPAR-alpha), were also measured in the CON and FO2 groups. Expression of mRNA for Delta(9)-desaturase was decreased (P < 0.05) 2.6-, 4.4-, and 4.9-fold in FO1, FO2(50), and FO2 compared with CON, respectively. Expression of Delta(9)-desaturase mRNA tended to be reduced (P = 0.09) by increasing FO from 1 to 2%, but was not affected by duration of supplementation (P > 0.24). Expression of mRNA for SREBP-1c was decreased 2-fold in FO2 compared with CON (P < 0.05), whereas expression of PPAR-alpha was not affected (P > 0.30). There was a positive relationship between Delta(9)-desaturase and SREBP-1c gene expression (P < 0.01), but the expression of both genes was negatively related to tissue concentrations of n-3 PUFA (P < 0.05) and positively related to concentration of n-6 PUFA (P < 0.01). Simultaneous enhancement of tissue concentrations of CLA and n-3 PUFA concentrations in bovine muscle may be hindered by negative interactions between n-3 PUFA and Delta(9)-desaturase gene expression, possibly mediated through reduced expression of SREBP-1c.

Authors+Show Affiliations

Teagasc, Animal Bioscience Centre, Grange, Dunsany, Co. Meath, Ireland. Sinead.Waters@teagasc.ieNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18791145

Citation

Waters, S M., et al. "Effect of Level and Duration of Dietary N-3 Polyunsaturated Fatty Acid Supplementation On the Transcriptional Regulation of Delta9-desaturase in Muscle of Beef Cattle." Journal of Animal Science, vol. 87, no. 1, 2009, pp. 244-52.
Waters SM, Kelly JP, O'Boyle P, et al. Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of Delta9-desaturase in muscle of beef cattle. J Anim Sci. 2009;87(1):244-52.
Waters, S. M., Kelly, J. P., O'Boyle, P., Moloney, A. P., & Kenny, D. A. (2009). Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of Delta9-desaturase in muscle of beef cattle. Journal of Animal Science, 87(1), 244-52. https://doi.org/10.2527/jas.2008-1005
Waters SM, et al. Effect of Level and Duration of Dietary N-3 Polyunsaturated Fatty Acid Supplementation On the Transcriptional Regulation of Delta9-desaturase in Muscle of Beef Cattle. J Anim Sci. 2009;87(1):244-52. PubMed PMID: 18791145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of Delta9-desaturase in muscle of beef cattle. AU - Waters,S M, AU - Kelly,J P, AU - O'Boyle,P, AU - Moloney,A P, AU - Kenny,D A, Y1 - 2008/09/12/ PY - 2008/9/16/pubmed PY - 2009/2/24/medline PY - 2008/9/16/entrez SP - 244 EP - 52 JF - Journal of animal science JO - J Anim Sci VL - 87 IS - 1 N2 - The objective of this study was to examine the effect of level and duration of feeding of an n-3 PUFA-enriched fish oil (FO) supplement in combination with soybean oil (SO) on the transcriptional regulation of Delta(9)-desaturase gene expression in bovine muscle. Beef bulls (n = 40) were assigned to 1 of 4 iso-lipid and isonitrogenous concentrate diets fed for ad libitum intake for a 100-d finishing period. Concentrates were supplemented with one of the following: 1) 6% SO (CON); 2) 6% SO + 1% FO (FO1); 3) 6% SO + 2% FO (FO2); or 4) 8% palmitic acid for the first 50 d and 6% SO + 2% FO for the second 50 d [FO2(50)]. Samples of LM were harvested and concentrations of fatty acids were measured. Total RNA was isolated and the gene expression of Delta(9)-desaturase was determined. The mRNA expression of putative regulators of Delta(9)-desaturase gene expression, sterol regulatory element binding protein-1c (SREBP-1c) and peroxisome proliferator activated receptor-alpha (PPAR-alpha), were also measured in the CON and FO2 groups. Expression of mRNA for Delta(9)-desaturase was decreased (P < 0.05) 2.6-, 4.4-, and 4.9-fold in FO1, FO2(50), and FO2 compared with CON, respectively. Expression of Delta(9)-desaturase mRNA tended to be reduced (P = 0.09) by increasing FO from 1 to 2%, but was not affected by duration of supplementation (P > 0.24). Expression of mRNA for SREBP-1c was decreased 2-fold in FO2 compared with CON (P < 0.05), whereas expression of PPAR-alpha was not affected (P > 0.30). There was a positive relationship between Delta(9)-desaturase and SREBP-1c gene expression (P < 0.01), but the expression of both genes was negatively related to tissue concentrations of n-3 PUFA (P < 0.05) and positively related to concentration of n-6 PUFA (P < 0.01). Simultaneous enhancement of tissue concentrations of CLA and n-3 PUFA concentrations in bovine muscle may be hindered by negative interactions between n-3 PUFA and Delta(9)-desaturase gene expression, possibly mediated through reduced expression of SREBP-1c. SN - 1525-3163 UR - https://www.unboundmedicine.com/medline/citation/18791145/Effect_of_level_and_duration_of_dietary_n_3_polyunsaturated_fatty_acid_supplementation_on_the_transcriptional_regulation_of_Delta9_desaturase_in_muscle_of_beef_cattle_ L2 - https://academic.oup.com/jas/article-lookup/doi/10.2527/jas.2008-1005 DB - PRIME DP - Unbound Medicine ER -