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Inflammatory cytokines augments TGF-beta1-induced epithelial-mesenchymal transition in A549 cells by up-regulating TbetaR-I.
Cell Motil Cytoskeleton. 2008 Dec; 65(12):935-44.CM

Abstract

Epithelial-mesenchymal transition (EMT) is believed to play an important role in fibrosis and tumor invasion. EMT can be induced in vitro cell culture by various stimuli including growth factors and matrix metalloproteinases. In this study, we report that cytomix (a mixture of IL-1beta, TNF-alpha and IFN-gamma) significantly enhances TGF-beta1-induced EMT in A549 cells as evidenced by acquisition of fibroblast-like cell shape, loss of E-cadherin, and reorganization of F-actin. IL-1beta or TNF-alpha alone can also augment TGF-beta1-induced EMT. However, a combination of IL-1beta and TNF-alpha or the cytomix is more potent to induce EMT. Cytomix, but not individual cytokine of IL-1beta, TNF-alpha or IFN-gamma, significantly up-regulates expression of TGF-beta receptor type I (TbetaR-I). Suppression of TbetaR-I, Smad2 or Smad3 by siRNA partially blocks EMT induction by cytomix plus TGF-beta1, indicating cytomix augments TGF-beta1-induced EMT through enhancing TbetaR-I and Smad signaling. These results indicate that inflammatory cytokines together with TGF-beta1 may play an important role in the development of fibrosis and tumor progress via the mechanism of epithelial-mesenchymal transition.

Authors+Show Affiliations

Pulmonary, Critical Care, Sleep and Allergy Medicine, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA. xdliu@unmc.edu

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18792103

Citation

Liu, Xiangde. "Inflammatory Cytokines Augments TGF-beta1-induced Epithelial-mesenchymal Transition in A549 Cells By Up-regulating TbetaR-I." Cell Motility and the Cytoskeleton, vol. 65, no. 12, 2008, pp. 935-44.
Liu X. Inflammatory cytokines augments TGF-beta1-induced epithelial-mesenchymal transition in A549 cells by up-regulating TbetaR-I. Cell Motil Cytoskeleton. 2008;65(12):935-44.
Liu, X. (2008). Inflammatory cytokines augments TGF-beta1-induced epithelial-mesenchymal transition in A549 cells by up-regulating TbetaR-I. Cell Motility and the Cytoskeleton, 65(12), 935-44. https://doi.org/10.1002/cm.20315
Liu X. Inflammatory Cytokines Augments TGF-beta1-induced Epithelial-mesenchymal Transition in A549 Cells By Up-regulating TbetaR-I. Cell Motil Cytoskeleton. 2008;65(12):935-44. PubMed PMID: 18792103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inflammatory cytokines augments TGF-beta1-induced epithelial-mesenchymal transition in A549 cells by up-regulating TbetaR-I. A1 - Liu,Xiangde, PY - 2008/9/16/pubmed PY - 2009/7/11/medline PY - 2008/9/16/entrez SP - 935 EP - 44 JF - Cell motility and the cytoskeleton JO - Cell Motil Cytoskeleton VL - 65 IS - 12 N2 - Epithelial-mesenchymal transition (EMT) is believed to play an important role in fibrosis and tumor invasion. EMT can be induced in vitro cell culture by various stimuli including growth factors and matrix metalloproteinases. In this study, we report that cytomix (a mixture of IL-1beta, TNF-alpha and IFN-gamma) significantly enhances TGF-beta1-induced EMT in A549 cells as evidenced by acquisition of fibroblast-like cell shape, loss of E-cadherin, and reorganization of F-actin. IL-1beta or TNF-alpha alone can also augment TGF-beta1-induced EMT. However, a combination of IL-1beta and TNF-alpha or the cytomix is more potent to induce EMT. Cytomix, but not individual cytokine of IL-1beta, TNF-alpha or IFN-gamma, significantly up-regulates expression of TGF-beta receptor type I (TbetaR-I). Suppression of TbetaR-I, Smad2 or Smad3 by siRNA partially blocks EMT induction by cytomix plus TGF-beta1, indicating cytomix augments TGF-beta1-induced EMT through enhancing TbetaR-I and Smad signaling. These results indicate that inflammatory cytokines together with TGF-beta1 may play an important role in the development of fibrosis and tumor progress via the mechanism of epithelial-mesenchymal transition. SN - 1097-0169 UR - https://www.unboundmedicine.com/medline/citation/18792103/Inflammatory_cytokines_augments_TGF_beta1_induced_epithelial_mesenchymal_transition_in_A549_cells_by_up_regulating_TbetaR_I_ L2 - https://doi.org/10.1002/cm.20315 DB - PRIME DP - Unbound Medicine ER -