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Cellular protection of morin against the oxidative stress induced by hydrogen peroxide.
Chem Biol Interact. 2009 Jan 15; 177(1):21-7.CB

Abstract

Flavonoids are a class of secondary metabolites abundantly found in fruits and vegetables. In addition, flavonoids have been reported as potent antioxidants with beneficial effects against oxidative stress-related diseases such as cancer, aging, and diabetes. The present study was carried out to investigate the cytoprotective effects of morin (2',3,4',5,7-pentahydroxyflavone), a member of the flavonoid group, against hydrogen peroxide (H(2)O(2))-induced DNA and lipid damage. Morin was found to prevent the cellular DNA damage induced by H(2)O(2) treatment, which is shown by the inhibition of 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation (a modified form of DNA base), inhibition of comet tail (a form of DNA strand breakage), and decrease of nuclear phospho histone H2A.X expression (a marker for DNA strand breakage). In addition, morin inhibited membrane lipid peroxidation, which is detected by inhibition of thiobarbituric acid reactive substance (TBARS) formation. Morin was found to scavenge the intracellular reactive oxygen species (ROS) generated by H(2)O(2) treatment in cells, which is detected by a spectrofluorometer, flow cytometry, and confocal microscopy after staining of 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Morin also induces an increase in the activity of catalase and protein expression. The results of this study suggest that morin protects cells from H(2)O(2)-induced damage by inhibiting ROS generation and by inducing catalase activation.

Authors+Show Affiliations

School of Medicine, Cheju National University, Jeju-si 690-756, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18793623

Citation

Zhang, Rui, et al. "Cellular Protection of Morin Against the Oxidative Stress Induced By Hydrogen Peroxide." Chemico-biological Interactions, vol. 177, no. 1, 2009, pp. 21-7.
Zhang R, Kang KA, Piao MJ, et al. Cellular protection of morin against the oxidative stress induced by hydrogen peroxide. Chem Biol Interact. 2009;177(1):21-7.
Zhang, R., Kang, K. A., Piao, M. J., Maeng, Y. H., Lee, K. H., Chang, W. Y., You, H. J., Kim, J. S., Kang, S. S., & Hyun, J. W. (2009). Cellular protection of morin against the oxidative stress induced by hydrogen peroxide. Chemico-biological Interactions, 177(1), 21-7. https://doi.org/10.1016/j.cbi.2008.08.009
Zhang R, et al. Cellular Protection of Morin Against the Oxidative Stress Induced By Hydrogen Peroxide. Chem Biol Interact. 2009 Jan 15;177(1):21-7. PubMed PMID: 18793623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular protection of morin against the oxidative stress induced by hydrogen peroxide. AU - Zhang,Rui, AU - Kang,Kyoung Ah, AU - Piao,Mei Jing, AU - Maeng,Young Hee, AU - Lee,Keun Hwa, AU - Chang,Weon Young, AU - You,Ho Jin, AU - Kim,Ju Sun, AU - Kang,Sam Sik, AU - Hyun,Jin Won, Y1 - 2008/08/27/ PY - 2008/04/17/received PY - 2008/08/11/revised PY - 2008/08/11/accepted PY - 2008/9/17/entrez PY - 2008/9/17/pubmed PY - 2009/1/16/medline SP - 21 EP - 7 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 177 IS - 1 N2 - Flavonoids are a class of secondary metabolites abundantly found in fruits and vegetables. In addition, flavonoids have been reported as potent antioxidants with beneficial effects against oxidative stress-related diseases such as cancer, aging, and diabetes. The present study was carried out to investigate the cytoprotective effects of morin (2',3,4',5,7-pentahydroxyflavone), a member of the flavonoid group, against hydrogen peroxide (H(2)O(2))-induced DNA and lipid damage. Morin was found to prevent the cellular DNA damage induced by H(2)O(2) treatment, which is shown by the inhibition of 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation (a modified form of DNA base), inhibition of comet tail (a form of DNA strand breakage), and decrease of nuclear phospho histone H2A.X expression (a marker for DNA strand breakage). In addition, morin inhibited membrane lipid peroxidation, which is detected by inhibition of thiobarbituric acid reactive substance (TBARS) formation. Morin was found to scavenge the intracellular reactive oxygen species (ROS) generated by H(2)O(2) treatment in cells, which is detected by a spectrofluorometer, flow cytometry, and confocal microscopy after staining of 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Morin also induces an increase in the activity of catalase and protein expression. The results of this study suggest that morin protects cells from H(2)O(2)-induced damage by inhibiting ROS generation and by inducing catalase activation. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/18793623/Cellular_protection_of_morin_against_the_oxidative_stress_induced_by_hydrogen_peroxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(08)00403-1 DB - PRIME DP - Unbound Medicine ER -