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[Effect of rapamycin in inducing naïve murine effector T cell convert to regulatory T cell].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008 Aug; 30(4):393-9.ZY

Abstract

OBJECTIVE

To study the effect of rapamycin in inducing naïve murine effector T cell (Teff) convert to regulatory T cell (Treg) in vitro.

METHODS

The forkhead box protein 3 (FoxP3) negative Teff were isolated and purified from the spleen and lymph node of C57 BL/6 murines aged 6-8 weeks, then Teff were cultured in three groups with mature dendritic cells (mDC), B cells, and plate coated Anti-CD3. In addition, the control wells and the test wells were prepared in each group, rapamycin were not added in the control wells but added in the test wells with concentrations of 1, 10, 50, and 100 nmol/L. Percentages of FoxP3 positive Treg were examined by flow cytometry after 4 days in Anti-CD3 group and after 6 days in the other two groups.

RESULTS

As shown by the flow cytometry, the percentages of FoxP3 positive Treg were as follows in three group: in the mDC group, it was 0.01% in the control well and 0.39%, 0.47%, 0.34%, and 0.26% in test wells; in B cell group, it was 0.01% in the control wells and 5.56%, 5.89%, 7.15%, and 4.72% in the test wells; in Anti-CD3 group, it was 0.93% in the control wells and 1.35%, 1.07%, 1.02%, and 1.19% in test wells. No significant difference was found between the test wells and control wells in the mDC group and Anti-CD3 group; however, the percentages of FoxP3 positive Treg was significantly different between the test wells and control wells in the B cell group (P < 0.01).

CONCLUSION

When B cell is acted as the antigen-presenting cell, rapamycin can effectively induce Teff convert to Treg in vitro.

Authors+Show Affiliations

Department of Hepatobiliary Surgery, Peking University People's Hospital, Peking University Center for Organ Transplantation, Beijing 100044, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18795608

Citation

Gao, Jie, et al. "[Effect of Rapamycin in Inducing Naïve Murine Effector T Cell Convert to Regulatory T Cell]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 30, no. 4, 2008, pp. 393-9.
Gao J, Chen JF, Wang ZH, et al. [Effect of rapamycin in inducing naïve murine effector T cell convert to regulatory T cell]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008;30(4):393-9.
Gao, J., Chen, J. F., Wang, Z. H., Song, Z. M., & Zhu, J. Y. (2008). [Effect of rapamycin in inducing naïve murine effector T cell convert to regulatory T cell]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 30(4), 393-9.
Gao J, et al. [Effect of Rapamycin in Inducing Naïve Murine Effector T Cell Convert to Regulatory T Cell]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008;30(4):393-9. PubMed PMID: 18795608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of rapamycin in inducing naïve murine effector T cell convert to regulatory T cell]. AU - Gao,Jie, AU - Chen,Jian-fei, AU - Wang,Zi-han, AU - Song,Zhao-min, AU - Zhu,Ji-ye, PY - 2008/9/18/pubmed PY - 2010/6/4/medline PY - 2008/9/18/entrez SP - 393 EP - 9 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 30 IS - 4 N2 - OBJECTIVE: To study the effect of rapamycin in inducing naïve murine effector T cell (Teff) convert to regulatory T cell (Treg) in vitro. METHODS: The forkhead box protein 3 (FoxP3) negative Teff were isolated and purified from the spleen and lymph node of C57 BL/6 murines aged 6-8 weeks, then Teff were cultured in three groups with mature dendritic cells (mDC), B cells, and plate coated Anti-CD3. In addition, the control wells and the test wells were prepared in each group, rapamycin were not added in the control wells but added in the test wells with concentrations of 1, 10, 50, and 100 nmol/L. Percentages of FoxP3 positive Treg were examined by flow cytometry after 4 days in Anti-CD3 group and after 6 days in the other two groups. RESULTS: As shown by the flow cytometry, the percentages of FoxP3 positive Treg were as follows in three group: in the mDC group, it was 0.01% in the control well and 0.39%, 0.47%, 0.34%, and 0.26% in test wells; in B cell group, it was 0.01% in the control wells and 5.56%, 5.89%, 7.15%, and 4.72% in the test wells; in Anti-CD3 group, it was 0.93% in the control wells and 1.35%, 1.07%, 1.02%, and 1.19% in test wells. No significant difference was found between the test wells and control wells in the mDC group and Anti-CD3 group; however, the percentages of FoxP3 positive Treg was significantly different between the test wells and control wells in the B cell group (P < 0.01). CONCLUSION: When B cell is acted as the antigen-presenting cell, rapamycin can effectively induce Teff convert to Treg in vitro. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/18795608/[Effect_of_rapamycin_in_inducing_naïve_murine_effector_T_cell_convert_to_regulatory_T_cell]_ L2 - http://www.actacams.com/article/2008/1000-503X/1000-503X-30-4-393.pdf DB - PRIME DP - Unbound Medicine ER -