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The adaptive response of anaerobically grown Saccharomyces cerevisiae to hydrogen peroxide is mediated by the Yap1 and Skn7 transcription factors.
FEMS Yeast Res. 2008 Dec; 8(8):1214-22.FY

Abstract

The molecular mechanisms involved in the ability of cells to adapt and respond to differing oxygen tensions are of great interest to the pharmaceutical, medical and fermentation industries. The transcriptional profiles reported in previous studies of cells grown under anaerobic, aerobic and dynamic growth conditions have shown significantly altered responses including induction of genes regulated by the oxidative stress transcription factor Yap1p when oxygen was present. The present study investigated the phenotypic changes that occur in cells when shifted from anaerobic to aerobic growth conditions and it was found through mutant analyses that the elevated activity of Yap1p during the shift was mediated by the phospholipid hydroperoxide-sensing protein encoded by GPX3. Cell viability and growth rate were unaffected even though anaerobically grown cells were found to be hypersensitive to low doses of the oxidative stress-inducing compound hydrogen peroxide (H(2)O(2)). Adaptation to H(2)O(2) treatment was demonstrated to occur when anaerobically grown wild-type cells were aerated for a short time that was reliant on the Yap1p and Skn7p transcription factors.

Links

Publisher Full Text
academic.oup.com
onlinelibrary.wiley.com
onlinelibrary.wiley.com

Authors+Show Affiliations

Beckhouse AG
Ramaciotti Centre for Gene Function Analysis, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Grant CM
No affiliation info available
Rogers PJ
No affiliation info available
Dawes IW
No affiliation info available
Higgins VJ
No affiliation info available

MeSH

Adaptation, PhysiologicalAnaerobiosisDNA-Binding ProteinsGene Expression Regulation, FungalHydrogen PeroxideMutationOxidative StressSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18795957

Citation

Beckhouse, Anthony G., et al. "The Adaptive Response of Anaerobically Grown Saccharomyces Cerevisiae to Hydrogen Peroxide Is Mediated By the Yap1 and Skn7 Transcription Factors." FEMS Yeast Research, vol. 8, no. 8, 2008, pp. 1214-22.
Beckhouse AG, Grant CM, Rogers PJ, et al. The adaptive response of anaerobically grown Saccharomyces cerevisiae to hydrogen peroxide is mediated by the Yap1 and Skn7 transcription factors. FEMS Yeast Res. 2008;8(8):1214-22.
Beckhouse, A. G., Grant, C. M., Rogers, P. J., Dawes, I. W., & Higgins, V. J. (2008). The adaptive response of anaerobically grown Saccharomyces cerevisiae to hydrogen peroxide is mediated by the Yap1 and Skn7 transcription factors. FEMS Yeast Research, 8(8), 1214-22. https://doi.org/10.1111/j.1567-1364.2008.00439.x
Beckhouse AG, et al. The Adaptive Response of Anaerobically Grown Saccharomyces Cerevisiae to Hydrogen Peroxide Is Mediated By the Yap1 and Skn7 Transcription Factors. FEMS Yeast Res. 2008;8(8):1214-22. PubMed PMID: 18795957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The adaptive response of anaerobically grown Saccharomyces cerevisiae to hydrogen peroxide is mediated by the Yap1 and Skn7 transcription factors. AU - Beckhouse,Anthony G, AU - Grant,Chris M, AU - Rogers,Peter J, AU - Dawes,Ian W, AU - Higgins,Vincent J, Y1 - 2008/09/15/ PY - 2008/9/18/pubmed PY - 2009/1/13/medline PY - 2008/9/18/entrez SP - 1214 EP - 22 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 8 N2 - The molecular mechanisms involved in the ability of cells to adapt and respond to differing oxygen tensions are of great interest to the pharmaceutical, medical and fermentation industries. The transcriptional profiles reported in previous studies of cells grown under anaerobic, aerobic and dynamic growth conditions have shown significantly altered responses including induction of genes regulated by the oxidative stress transcription factor Yap1p when oxygen was present. The present study investigated the phenotypic changes that occur in cells when shifted from anaerobic to aerobic growth conditions and it was found through mutant analyses that the elevated activity of Yap1p during the shift was mediated by the phospholipid hydroperoxide-sensing protein encoded by GPX3. Cell viability and growth rate were unaffected even though anaerobically grown cells were found to be hypersensitive to low doses of the oxidative stress-inducing compound hydrogen peroxide (H(2)O(2)). Adaptation to H(2)O(2) treatment was demonstrated to occur when anaerobically grown wild-type cells were aerated for a short time that was reliant on the Yap1p and Skn7p transcription factors. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18795957/The_adaptive_response_of_anaerobically_grown_Saccharomyces_cerevisiae_to_hydrogen_peroxide_is_mediated_by_the_Yap1_and_Skn7_transcription_factors_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00439.x DB - PRIME DP - Unbound Medicine ER -