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Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall.
Appl Microbiol Biotechnol. 2008 Dec; 81(3):543-50.AM

Abstract

Freeze-dried samples of four Saccharomyces cerevisiae strains, namely, FL01, FL03, 2.0016, and 2.1424, were subjected to spaceflight. After the satellite's landing on Earth, the samples were recovered and changes in yeast cell wall were analyzed. Spaceflight strains of all S. cerevisiae strains showed significant changes in cell wall thickness (P < 0.05). One mutant of S. cerevisiae 2.0016 with increased biomass, cell wall thickness, and cell wall glucan was isolated (P < 0.05). The spaceflight mutant of S. cerevisiae 2.0016 showed 46.7%, 62.6%, and 146.0% increment in biomass, cell wall thickness and beta-glucan content, respectively, when compared to the ground strain. Moreover, growth curve analysis showed spaceflight S. cerevisiae 2.0016 had a faster growth rate, shorter lag phase periods, higher final biomass, and higher content of beta-glucan. Genetic stability analysis showed that prolonged subculturing of spaceflight strain S. cerevisiae 2.0016 did not lead to the appearance of variants, indicating that the genetic stability of S. cerevisiae 2.0016 mutant could be sufficient for its exploitation of beta-glucan production.

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Authors+Show Affiliations

Liu HZ
Institute of Agro-food Science and Technology, Chinese Academy of Agriculture Sciences, Beijing, 100193, People's Republic of China. lhz0416@hotmail.com
Wang Q
No affiliation info available
Liu XY
No affiliation info available
Tan SS
No affiliation info available

MeSH

BiomassCell WallGlucansMutationPolysaccharidesSaccharomyces cerevisiaeSpace Flight

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18797865

Citation

Liu, Hong-Zhi, et al. "Effects of Spaceflight On Polysaccharides of Saccharomyces Cerevisiae Cell Wall." Applied Microbiology and Biotechnology, vol. 81, no. 3, 2008, pp. 543-50.
Liu HZ, Wang Q, Liu XY, et al. Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall. Appl Microbiol Biotechnol. 2008;81(3):543-50.
Liu, H. Z., Wang, Q., Liu, X. Y., & Tan, S. S. (2008). Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall. Applied Microbiology and Biotechnology, 81(3), 543-50. https://doi.org/10.1007/s00253-008-1692-y
Liu HZ, et al. Effects of Spaceflight On Polysaccharides of Saccharomyces Cerevisiae Cell Wall. Appl Microbiol Biotechnol. 2008;81(3):543-50. PubMed PMID: 18797865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall. AU - Liu,Hong-Zhi, AU - Wang,Qiang, AU - Liu,Xiao-Yong, AU - Tan,Sze-Sze, Y1 - 2008/09/17/ PY - 2008/06/12/received PY - 2008/08/26/accepted PY - 2008/08/24/revised PY - 2008/9/18/pubmed PY - 2008/12/17/medline PY - 2008/9/18/entrez SP - 543 EP - 50 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 81 IS - 3 N2 - Freeze-dried samples of four Saccharomyces cerevisiae strains, namely, FL01, FL03, 2.0016, and 2.1424, were subjected to spaceflight. After the satellite's landing on Earth, the samples were recovered and changes in yeast cell wall were analyzed. Spaceflight strains of all S. cerevisiae strains showed significant changes in cell wall thickness (P < 0.05). One mutant of S. cerevisiae 2.0016 with increased biomass, cell wall thickness, and cell wall glucan was isolated (P < 0.05). The spaceflight mutant of S. cerevisiae 2.0016 showed 46.7%, 62.6%, and 146.0% increment in biomass, cell wall thickness and beta-glucan content, respectively, when compared to the ground strain. Moreover, growth curve analysis showed spaceflight S. cerevisiae 2.0016 had a faster growth rate, shorter lag phase periods, higher final biomass, and higher content of beta-glucan. Genetic stability analysis showed that prolonged subculturing of spaceflight strain S. cerevisiae 2.0016 did not lead to the appearance of variants, indicating that the genetic stability of S. cerevisiae 2.0016 mutant could be sufficient for its exploitation of beta-glucan production. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/18797865/Effects_of_spaceflight_on_polysaccharides_of_Saccharomyces_cerevisiae_cell_wall_ L2 - https://dx.doi.org/10.1007/s00253-008-1692-y DB - PRIME DP - Unbound Medicine ER -