Tags

Type your tag names separated by a space and hit enter

Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium.
Comp Immunol Microbiol Infect Dis. 2010 Mar; 33(2):161-74.CI

Abstract

The innate immune system has the basic function of identifying and eradicating microbial invaders and alerting the adaptative immune system to their presence. In this study, the porcine intestinal innate immune response was evaluated by analysing the expression of TLRs, cytokines and chemokines in two porcine epithelial cell lines from different regions: IPEC-J2 (jejunum) and IPI-2I (ileum). Both cells lines were stimulated with 1microg of LPS from Salmonella typhimurium. RNA was collected at 30min, 1, 2, 3 and 4h after treatment. Expression of TLR-1, -2, -3, -4, -6, -8, -9, -10, TNF-alpha, IL-1beta, -8 and MCP-1 was quantified relative to the quantity of Cyclophilin-A mRNA using real-time quantitative PCR (RTQ-PCR). The results obtained show up differences in the gene expression between both cell lines IPEC-J2 and IPI-2I as response to LPS from S. typhimurium during the activation time, which may suggest an in vivo variability in the innate immune response against pathogens in different regions of the host's gut.

Authors+Show Affiliations

Departamento de Genética, Universidad de Córdoba, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18799216

Citation

Arce, C, et al. "Innate Immune Activation of Swine Intestinal Epithelial Cell Lines (IPEC-J2 and IPI-2I) in Response to LPS From Salmonella Typhimurium." Comparative Immunology, Microbiology and Infectious Diseases, vol. 33, no. 2, 2010, pp. 161-74.
Arce C, Ramírez-Boo M, Lucena C, et al. Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium. Comp Immunol Microbiol Infect Dis. 2010;33(2):161-74.
Arce, C., Ramírez-Boo, M., Lucena, C., & Garrido, J. J. (2010). Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium. Comparative Immunology, Microbiology and Infectious Diseases, 33(2), 161-74. https://doi.org/10.1016/j.cimid.2008.08.003
Arce C, et al. Innate Immune Activation of Swine Intestinal Epithelial Cell Lines (IPEC-J2 and IPI-2I) in Response to LPS From Salmonella Typhimurium. Comp Immunol Microbiol Infect Dis. 2010;33(2):161-74. PubMed PMID: 18799216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium. AU - Arce,C, AU - Ramírez-Boo,M, AU - Lucena,C, AU - Garrido,J J, Y1 - 2008/09/16/ PY - 2008/08/09/accepted PY - 2008/9/19/pubmed PY - 2010/5/12/medline PY - 2008/9/19/entrez SP - 161 EP - 74 JF - Comparative immunology, microbiology and infectious diseases JO - Comp Immunol Microbiol Infect Dis VL - 33 IS - 2 N2 - The innate immune system has the basic function of identifying and eradicating microbial invaders and alerting the adaptative immune system to their presence. In this study, the porcine intestinal innate immune response was evaluated by analysing the expression of TLRs, cytokines and chemokines in two porcine epithelial cell lines from different regions: IPEC-J2 (jejunum) and IPI-2I (ileum). Both cells lines were stimulated with 1microg of LPS from Salmonella typhimurium. RNA was collected at 30min, 1, 2, 3 and 4h after treatment. Expression of TLR-1, -2, -3, -4, -6, -8, -9, -10, TNF-alpha, IL-1beta, -8 and MCP-1 was quantified relative to the quantity of Cyclophilin-A mRNA using real-time quantitative PCR (RTQ-PCR). The results obtained show up differences in the gene expression between both cell lines IPEC-J2 and IPI-2I as response to LPS from S. typhimurium during the activation time, which may suggest an in vivo variability in the innate immune response against pathogens in different regions of the host's gut. SN - 1878-1667 UR - https://www.unboundmedicine.com/medline/citation/18799216/Innate_immune_activation_of_swine_intestinal_epithelial_cell_lines__IPEC_J2_and_IPI_2I__in_response_to_LPS_from_Salmonella_typhimurium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-9571(08)00053-2 DB - PRIME DP - Unbound Medicine ER -