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A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode.
Biosens Bioelectron. 2009 Jan 01; 24(5):1389-93.BB

Abstract

A novel reagentless immunosensor was fabricated by immobilization of redox mediator 3,3',5,5'-tetramethylbenzidine (TMB) on the Nafion (Nf) film modified glassy carbon electrode. Gold nanoparticles were assembled onto the TMB/Nafion film modified electrode to provide active sites for the immobilization of antibody molecules. The antibody (anti-MIgG), in the present study, was fixed on the electrode for the rapid detection of antigen molecules (MIgG as a model analyte). The results showed that the immunosensor based on the immobilized TMB redox mediator exhibited good electrochemical response. A good linear relationship between peak current and the concentration of the MIgG was obtained in the concentration range from 4 to 120ng/mL. The detection limit was estimated to be 1ng/ml. Under the optimized conditions, the immunosensor exhibits good sensitivity, reproducibility and stability.

Authors+Show Affiliations

Institute of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215123, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18799302

Citation

Wu, Ying, et al. "A Novel Reagentless Amperometric Immunosensor Based On Gold nanoparticles/TMB/Nafion-modified Electrode." Biosensors & Bioelectronics, vol. 24, no. 5, 2009, pp. 1389-93.
Wu Y, Zheng J, Li Z, et al. A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode. Biosens Bioelectron. 2009;24(5):1389-93.
Wu, Y., Zheng, J., Li, Z., Zhao, Y., & Zhang, Y. (2009). A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode. Biosensors & Bioelectronics, 24(5), 1389-93. https://doi.org/10.1016/j.bios.2008.07.075
Wu Y, et al. A Novel Reagentless Amperometric Immunosensor Based On Gold nanoparticles/TMB/Nafion-modified Electrode. Biosens Bioelectron. 2009 Jan 1;24(5):1389-93. PubMed PMID: 18799302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode. AU - Wu,Ying, AU - Zheng,Junwei, AU - Li,Zheng, AU - Zhao,Yanrong, AU - Zhang,Yu, Y1 - 2008/08/13/ PY - 2008/04/14/received PY - 2008/07/30/revised PY - 2008/07/31/accepted PY - 2008/9/19/pubmed PY - 2009/3/10/medline PY - 2008/9/19/entrez SP - 1389 EP - 93 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 5 N2 - A novel reagentless immunosensor was fabricated by immobilization of redox mediator 3,3',5,5'-tetramethylbenzidine (TMB) on the Nafion (Nf) film modified glassy carbon electrode. Gold nanoparticles were assembled onto the TMB/Nafion film modified electrode to provide active sites for the immobilization of antibody molecules. The antibody (anti-MIgG), in the present study, was fixed on the electrode for the rapid detection of antigen molecules (MIgG as a model analyte). The results showed that the immunosensor based on the immobilized TMB redox mediator exhibited good electrochemical response. A good linear relationship between peak current and the concentration of the MIgG was obtained in the concentration range from 4 to 120ng/mL. The detection limit was estimated to be 1ng/ml. Under the optimized conditions, the immunosensor exhibits good sensitivity, reproducibility and stability. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18799302/A_novel_reagentless_amperometric_immunosensor_based_on_gold_nanoparticles/TMB/Nafion_modified_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00430-2 DB - PRIME DP - Unbound Medicine ER -