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Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea.
BMC Complement Altern Med. 2008 Sep 20; 8:53.BC

Abstract

BACKGROUND

In South Africa, Calpurnia aurea (Ait.) Benth is used to destroy lice and to relieve itches, to destroy maggots and to treat allergic rashes, particularly those caused by caterpillars. Antioxidants play an important role protecting against damage by reactive oxygen species. Plants containing flavonoids have been reported to possess strong antioxidant properties.

METHODS

The antibacterial, antioxidant activities and phenolic contents of the methanol extracts of the leaves and stems of Calpurnia aurea were evaluated using in vitro standard methods. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the stem extract of Calpurnia aurea were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Laboratory isolates of 10 bacteria species which included five Gram-positive and five Gram-negative strains were used to assay for antibacterial activity of this plant.

RESULTS

The results from this study showed that the antioxidant activities of the stem extract of Calpurnia aurea as determined by the total phenol, flavonoids, and FRAP methods were higher than that of the leaves. On the other hand, the leaf extract of the plant has higher level of total flavonols and proanthocyanidins. The leaf extract also has higher radical scavenging activity as shown in 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), and 2,2¿-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. The leaf extract showed activity against seven of the bacterial organisms.

CONCLUSION

The results from this study indicate that the leaves and stem extracts of Calpurnia aurea possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as primary antioxidants. Although, the antibacterial properties of Calpurnia aurea are not as effective as the standard drugs- Chloramphenicol and Streptomycin, they still possess some activity against bacterial strains used in this study. Calpurnia aurea may therefore be a good candidate for functional foods as well as pharmaceutical plant-based products.

Authors+Show Affiliations

Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria. adedapo3a@yahoo.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18803865

Citation

Adedapo, Adeolu A., et al. "Antibacterial and Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Calpurnia Aurea." BMC Complementary and Alternative Medicine, vol. 8, 2008, p. 53.
Adedapo AA, Jimoh FO, Koduru S, et al. Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complement Altern Med. 2008;8:53.
Adedapo, A. A., Jimoh, F. O., Koduru, S., Afolayan, A. J., & Masika, P. J. (2008). Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complementary and Alternative Medicine, 8, 53. https://doi.org/10.1186/1472-6882-8-53
Adedapo AA, et al. Antibacterial and Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Calpurnia Aurea. BMC Complement Altern Med. 2008 Sep 20;8:53. PubMed PMID: 18803865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. AU - Adedapo,Adeolu A, AU - Jimoh,Florence O, AU - Koduru,Srinivas, AU - Afolayan,Anthony J, AU - Masika,Patrick J, Y1 - 2008/09/20/ PY - 2008/06/06/received PY - 2008/09/20/accepted PY - 2008/9/23/pubmed PY - 2008/12/17/medline PY - 2008/9/23/entrez SP - 53 EP - 53 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 8 N2 - BACKGROUND: In South Africa, Calpurnia aurea (Ait.) Benth is used to destroy lice and to relieve itches, to destroy maggots and to treat allergic rashes, particularly those caused by caterpillars. Antioxidants play an important role protecting against damage by reactive oxygen species. Plants containing flavonoids have been reported to possess strong antioxidant properties. METHODS: The antibacterial, antioxidant activities and phenolic contents of the methanol extracts of the leaves and stems of Calpurnia aurea were evaluated using in vitro standard methods. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the stem extract of Calpurnia aurea were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Laboratory isolates of 10 bacteria species which included five Gram-positive and five Gram-negative strains were used to assay for antibacterial activity of this plant. RESULTS: The results from this study showed that the antioxidant activities of the stem extract of Calpurnia aurea as determined by the total phenol, flavonoids, and FRAP methods were higher than that of the leaves. On the other hand, the leaf extract of the plant has higher level of total flavonols and proanthocyanidins. The leaf extract also has higher radical scavenging activity as shown in 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), and 2,2¿-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. The leaf extract showed activity against seven of the bacterial organisms. CONCLUSION: The results from this study indicate that the leaves and stem extracts of Calpurnia aurea possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as primary antioxidants. Although, the antibacterial properties of Calpurnia aurea are not as effective as the standard drugs- Chloramphenicol and Streptomycin, they still possess some activity against bacterial strains used in this study. Calpurnia aurea may therefore be a good candidate for functional foods as well as pharmaceutical plant-based products. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/18803865/Antibacterial_and_antioxidant_properties_of_the_methanol_extracts_of_the_leaves_and_stems_of_Calpurnia_aurea_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-8-53 DB - PRIME DP - Unbound Medicine ER -