Tags

Type your tag names separated by a space and hit enter

Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches.
Eukaryot Cell. 2008 Nov; 7(11):1980-93.EC

Abstract

Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin in their cell walls. Here we present the first study of the cell wall of the oomycete Aphanomyces euteiches, a major parasite of legume plants. Biochemical analyses demonstrated the presence of ca. 10% N-acetyl-D-glucosamine (GlcNAc) in the cell wall. Further characterization of the GlcNAc-containing material revealed that it corresponds to noncrystalline chitosaccharides associated with glucans, rather than to chitin per se. Two putative chitin synthase (CHS) genes were identified by data mining of an A. euteiches expressed sequence tag collection and Southern blot analysis, and full-length cDNA sequences of both genes were obtained. Phylogeny analysis indicated that oomycete CHS diversification occurred before the divergence of the major oomycete lineages. Remarkably, lectin labeling showed that the Aphanomyces euteiches chitosaccharides are exposed at the cell wall surface, and study of the effect of the CHS inhibitor nikkomycin Z demonstrated that they are involved in cell wall function. These data open new perspectives for the development of antioomycete drugs and further studies of the molecular mechanisms involved in the recognition of pathogenic oomycetes by the host plants.

Authors+Show Affiliations

UMR 5546, CNRS-Université Paul-Sabatier, 24 Chemin de Borde-Rouge, BP 42617, Auzeville, F-31326 Castanet-Tolosan, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18806214

Citation

Badreddine, Ilham, et al. "Cell Wall Chitosaccharides Are Essential Components and Exposed Patterns of the Phytopathogenic Oomycete Aphanomyces Euteiches." Eukaryotic Cell, vol. 7, no. 11, 2008, pp. 1980-93.
Badreddine I, Lafitte C, Heux L, et al. Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches. Eukaryotic Cell. 2008;7(11):1980-93.
Badreddine, I., Lafitte, C., Heux, L., Skandalis, N., Spanou, Z., Martinez, Y., Esquerré-Tugayé, M. T., Bulone, V., Dumas, B., & Bottin, A. (2008). Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches. Eukaryotic Cell, 7(11), 1980-93. https://doi.org/10.1128/EC.00091-08
Badreddine I, et al. Cell Wall Chitosaccharides Are Essential Components and Exposed Patterns of the Phytopathogenic Oomycete Aphanomyces Euteiches. Eukaryotic Cell. 2008;7(11):1980-93. PubMed PMID: 18806214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches. AU - Badreddine,Ilham, AU - Lafitte,Claude, AU - Heux,Laurent, AU - Skandalis,Nicholas, AU - Spanou,Zacharoula, AU - Martinez,Yves, AU - Esquerré-Tugayé,Marie-Thérèse, AU - Bulone,Vincent, AU - Dumas,Bernard, AU - Bottin,Arnaud, Y1 - 2008/09/19/ PY - 2008/9/23/pubmed PY - 2008/12/17/medline PY - 2008/9/23/entrez SP - 1980 EP - 93 JF - Eukaryotic cell JO - Eukaryotic Cell VL - 7 IS - 11 N2 - Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin in their cell walls. Here we present the first study of the cell wall of the oomycete Aphanomyces euteiches, a major parasite of legume plants. Biochemical analyses demonstrated the presence of ca. 10% N-acetyl-D-glucosamine (GlcNAc) in the cell wall. Further characterization of the GlcNAc-containing material revealed that it corresponds to noncrystalline chitosaccharides associated with glucans, rather than to chitin per se. Two putative chitin synthase (CHS) genes were identified by data mining of an A. euteiches expressed sequence tag collection and Southern blot analysis, and full-length cDNA sequences of both genes were obtained. Phylogeny analysis indicated that oomycete CHS diversification occurred before the divergence of the major oomycete lineages. Remarkably, lectin labeling showed that the Aphanomyces euteiches chitosaccharides are exposed at the cell wall surface, and study of the effect of the CHS inhibitor nikkomycin Z demonstrated that they are involved in cell wall function. These data open new perspectives for the development of antioomycete drugs and further studies of the molecular mechanisms involved in the recognition of pathogenic oomycetes by the host plants. SN - 1535-9786 UR - https://www.unboundmedicine.com/medline/citation/18806214/Cell_wall_chitosaccharides_are_essential_components_and_exposed_patterns_of_the_phytopathogenic_oomycete_Aphanomyces_euteiches_ L2 - http://ec.asm.org/cgi/pmidlookup?view=long&pmid=18806214 DB - PRIME DP - Unbound Medicine ER -