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[Effect of scalp-acupuncture on plasma and cerebral TNF-alpha and IL-1beta contents in acute cerebral ischemia/reperfusion injury rats].
Zhen Ci Yan Jiu 2008; 33(3):173-8ZC

Abstract

OBJECTIVE

To explore the underlying mechanism of scalp-acupuncture therapy in the treatment of acute cerebral ischemia (ACI) in the rat.

METHODS

A total of 140 SD female rats were randomly assigned to sham-operation (n=20), model (n=60), scalp-acupuncture (SA, n=60) groups, and the later two groups were further divided into 24 h, 48 h and 72 h subgroups separately, with 20 cases in each. Among them, 70 rats were used for cerebral tissue section, and the other 70 cases for homogenating cerebral tissue. ACI model was established by occlusion of the middle cerebral artery (MCAO) for 1 h and reperfusion. EA (2/100 Hz, 2 mA) was applied to bilateral "Dingnie Houxiexian" (MS 7) and "Dingnie Qianxiexian" (MS 6) for 20 min, once daily for 1 d, 2 d and 3 d respectively. The rat's neurological severity score (NSS) was assessed before and after EA. Blood and brain tissue were sampled for detecting TNF-alpha and IL-1beta contents respectively with enzyme-linked immunosorbent assay. Haematoxylin-eosine (H&E) staining method was used for displaying the inflammatory cells in the ischemic brain tissue.

RESULTS

(1) After ACI, NSS at each time-point increased significantly, while compared with model group, NSS of SA group decreased apparently 72 h after ACI (P<0.01). Compared with the corresponding time-points of sham-operation group, the number of inflammatory cells, plasma and cerebral TNF-alpha and IL-1beta contents at 24 h, 48 h and 72 h in model group increased considerably (P<0.01, 0.05). In comparison with the corresponding time-points of model group, the number of inflammatory cells at 48 h and 72 h, plasma and cerebral TNF-alpha and IL-1beta contents at 72 h in SA group declined significantly (P<0.01).

CONCLUSION

Scalp-acupuncture can relieve inflammatory cell infiltration, and reduce plasma and cerebral TNF-alpha and IL-1beta contents in ACI rats, which may contribute to its effect in promoting neurofunctional recovery.

Authors+Show Affiliations

Department of Acu-moxibustion, Wuhan Integrated Chinese and Western Medicine Hospital, Wuhan 430022, China. bluesea-zl2000@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18807719

Citation

Zhou, Li, et al. "[Effect of Scalp-acupuncture On Plasma and Cerebral TNF-alpha and IL-1beta Contents in Acute Cerebral Ischemia/reperfusion Injury Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 33, no. 3, 2008, pp. 173-8.
Zhou L, Zhang HX, Liu LG, et al. [Effect of scalp-acupuncture on plasma and cerebral TNF-alpha and IL-1beta contents in acute cerebral ischemia/reperfusion injury rats]. Zhen Ci Yan Jiu. 2008;33(3):173-8.
Zhou, L., Zhang, H. X., Liu, L. G., Huang, H., Li, X., & Yang, M. (2008). [Effect of scalp-acupuncture on plasma and cerebral TNF-alpha and IL-1beta contents in acute cerebral ischemia/reperfusion injury rats]. Zhen Ci Yan Jiu = Acupuncture Research, 33(3), pp. 173-8.
Zhou L, et al. [Effect of Scalp-acupuncture On Plasma and Cerebral TNF-alpha and IL-1beta Contents in Acute Cerebral Ischemia/reperfusion Injury Rats]. Zhen Ci Yan Jiu. 2008;33(3):173-8. PubMed PMID: 18807719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of scalp-acupuncture on plasma and cerebral TNF-alpha and IL-1beta contents in acute cerebral ischemia/reperfusion injury rats]. AU - Zhou,Li, AU - Zhang,Hong-Xing, AU - Liu,Ling-Guang, AU - Huang,Hao, AU - Li,Xuan, AU - Yang,Min, PY - 2008/9/24/pubmed PY - 2008/10/24/medline PY - 2008/9/24/entrez SP - 173 EP - 8 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 33 IS - 3 N2 - OBJECTIVE: To explore the underlying mechanism of scalp-acupuncture therapy in the treatment of acute cerebral ischemia (ACI) in the rat. METHODS: A total of 140 SD female rats were randomly assigned to sham-operation (n=20), model (n=60), scalp-acupuncture (SA, n=60) groups, and the later two groups were further divided into 24 h, 48 h and 72 h subgroups separately, with 20 cases in each. Among them, 70 rats were used for cerebral tissue section, and the other 70 cases for homogenating cerebral tissue. ACI model was established by occlusion of the middle cerebral artery (MCAO) for 1 h and reperfusion. EA (2/100 Hz, 2 mA) was applied to bilateral "Dingnie Houxiexian" (MS 7) and "Dingnie Qianxiexian" (MS 6) for 20 min, once daily for 1 d, 2 d and 3 d respectively. The rat's neurological severity score (NSS) was assessed before and after EA. Blood and brain tissue were sampled for detecting TNF-alpha and IL-1beta contents respectively with enzyme-linked immunosorbent assay. Haematoxylin-eosine (H&E) staining method was used for displaying the inflammatory cells in the ischemic brain tissue. RESULTS: (1) After ACI, NSS at each time-point increased significantly, while compared with model group, NSS of SA group decreased apparently 72 h after ACI (P<0.01). Compared with the corresponding time-points of sham-operation group, the number of inflammatory cells, plasma and cerebral TNF-alpha and IL-1beta contents at 24 h, 48 h and 72 h in model group increased considerably (P<0.01, 0.05). In comparison with the corresponding time-points of model group, the number of inflammatory cells at 48 h and 72 h, plasma and cerebral TNF-alpha and IL-1beta contents at 72 h in SA group declined significantly (P<0.01). CONCLUSION: Scalp-acupuncture can relieve inflammatory cell infiltration, and reduce plasma and cerebral TNF-alpha and IL-1beta contents in ACI rats, which may contribute to its effect in promoting neurofunctional recovery. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/18807719/[Effect_of_scalp_acupuncture_on_plasma_and_cerebral_TNF_alpha_and_IL_1beta_contents_in_acute_cerebral_ischemia/reperfusion_injury_rats]_ L2 - https://medlineplus.gov/acupuncture.html DB - PRIME DP - Unbound Medicine ER -