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The proteomic response of Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation.
J Proteome Res. 2008 Nov; 7(11):4766-74.JP

Abstract

Ethanol yield by Saccharomyces cerevisiae in very high glucose (VHG) media with an amino acid supplement was investigated. Amino acid supplementation led to positive cell responses, including reduced lag time and increased cell viability in VHG media. A quantitative shotgun proteomic analysis was used to understand how amino acid supplemented S. cerevisiae responds to high osmotic conditions. iTRAQ data revealed that most proteins involved in glycolysis and pentose phosphate pathways were up-regulated under high glucose shock. Reactivation of amino acid metabolism was also observed at the end of the lag phase. The relative abundance of most identified proteins, including aminoacyl-tRNA biosynthesis proteins, and heat-shock proteins, remained unchanged in the hours immediately following application of glucose shock. However, the expression of these proteins increased significantly at the end of the lag phase. Furthermore, the up-regulation of trehalose and glycogen biosynthesis proteins, first maintaining then latterly increasing glycolysis pathway activity was also observed. This was verified by enhanced ethanol yields at 10 and 12 h (0.43 and 0.45 g ethanol/g glucose) compared to 2 h (0.32 g ethanol/g glucose). These data combined with relevant metabolite measurements demonstrates that enhanced ethanol fermentation under VHG conditions can be achieved with the aid of amino acid supplementation.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Pham TK
Biological and Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom.
Wright PC
No affiliation info available

MeSH

Amino AcidsCell SurvivalDose-Response Relationship, DrugEthanolFermentationGene Expression Regulation, FungalGlucoseGlycogenGlycolysisModels, BiologicalProteomeProteomicsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrehalose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18808174

Citation

Pham, Trong Khoa, and Phillip C. Wright. "The Proteomic Response of Saccharomyces Cerevisiae in Very High Glucose Conditions With Amino Acid Supplementation." Journal of Proteome Research, vol. 7, no. 11, 2008, pp. 4766-74.
Pham TK, Wright PC. The proteomic response of Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation. J Proteome Res. 2008;7(11):4766-74.
Pham, T. K., & Wright, P. C. (2008). The proteomic response of Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation. Journal of Proteome Research, 7(11), 4766-74. https://doi.org/10.1021/pr800331s
Pham TK, Wright PC. The Proteomic Response of Saccharomyces Cerevisiae in Very High Glucose Conditions With Amino Acid Supplementation. J Proteome Res. 2008;7(11):4766-74. PubMed PMID: 18808174.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The proteomic response of Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation. AU - Pham,Trong Khoa, AU - Wright,Phillip C, Y1 - 2008/09/23/ PY - 2008/9/24/pubmed PY - 2009/1/8/medline PY - 2008/9/24/entrez SP - 4766 EP - 74 JF - Journal of proteome research JO - J Proteome Res VL - 7 IS - 11 N2 - Ethanol yield by Saccharomyces cerevisiae in very high glucose (VHG) media with an amino acid supplement was investigated. Amino acid supplementation led to positive cell responses, including reduced lag time and increased cell viability in VHG media. A quantitative shotgun proteomic analysis was used to understand how amino acid supplemented S. cerevisiae responds to high osmotic conditions. iTRAQ data revealed that most proteins involved in glycolysis and pentose phosphate pathways were up-regulated under high glucose shock. Reactivation of amino acid metabolism was also observed at the end of the lag phase. The relative abundance of most identified proteins, including aminoacyl-tRNA biosynthesis proteins, and heat-shock proteins, remained unchanged in the hours immediately following application of glucose shock. However, the expression of these proteins increased significantly at the end of the lag phase. Furthermore, the up-regulation of trehalose and glycogen biosynthesis proteins, first maintaining then latterly increasing glycolysis pathway activity was also observed. This was verified by enhanced ethanol yields at 10 and 12 h (0.43 and 0.45 g ethanol/g glucose) compared to 2 h (0.32 g ethanol/g glucose). These data combined with relevant metabolite measurements demonstrates that enhanced ethanol fermentation under VHG conditions can be achieved with the aid of amino acid supplementation. SN - 1535-3893 UR - https://www.unboundmedicine.com/medline/citation/18808174/The_proteomic_response_of_Saccharomyces_cerevisiae_in_very_high_glucose_conditions_with_amino_acid_supplementation_ L2 - https://doi.org/10.1021/pr800331s DB - PRIME DP - Unbound Medicine ER -