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Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter.
Plant J. 2009 Feb; 57(3):413-25.PJ

Abstract

The plant genome contains a large number of disease resistance (R) genes that have evolved through diverse mechanisms. Here, we report that a long terminal repeat (LTR) retrotransposon contributed to the evolution of the rice blast resistance gene Pit. Pit confers race-specific resistance against the fungal pathogen Magnaporthe grisea, and is a member of the nucleotide-binding site leucine-rich repeat (NBS-LRR) family of R genes. Compared with the non-functional allele Pit(Npb), the functional allele Pit(K59) contains four amino acid substitutions, and has the LTR retrotransposon Renovator inserted upstream. Pathogenesis assays using chimeric constructs carrying the various regions of Pit(K59) and Pit(Npb) suggest that amino acid substitutions might have a potential effect in Pit resistance; more importantly, the upregulated promoter activity conferred by the Renovator sequence is essential for Pit function. Our data suggest that transposon-mediated transcriptional activation may play an important role in the refunctionalization of additional 'sleeping' R genes in the plant genome.

Authors+Show Affiliations

National Agricultural Research Center, National Agriculture and Food Research Organization, 1-2-1 Inada, Jo-etsu, Niigata 943 0193, Japan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18808453

Citation

Hayashi, Keiko, and Hitoshi Yoshida. "Refunctionalization of the Ancient Rice Blast Disease Resistance Gene Pit By the Recruitment of a Retrotransposon as a Promoter." The Plant Journal : for Cell and Molecular Biology, vol. 57, no. 3, 2009, pp. 413-25.
Hayashi K, Yoshida H. Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter. Plant J. 2009;57(3):413-25.
Hayashi, K., & Yoshida, H. (2009). Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter. The Plant Journal : for Cell and Molecular Biology, 57(3), 413-25. https://doi.org/10.1111/j.1365-313X.2008.03694.x
Hayashi K, Yoshida H. Refunctionalization of the Ancient Rice Blast Disease Resistance Gene Pit By the Recruitment of a Retrotransposon as a Promoter. Plant J. 2009;57(3):413-25. PubMed PMID: 18808453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter. AU - Hayashi,Keiko, AU - Yoshida,Hitoshi, Y1 - 2008/09/21/ PY - 2008/9/24/pubmed PY - 2009/2/27/medline PY - 2008/9/24/entrez SP - 413 EP - 25 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 57 IS - 3 N2 - The plant genome contains a large number of disease resistance (R) genes that have evolved through diverse mechanisms. Here, we report that a long terminal repeat (LTR) retrotransposon contributed to the evolution of the rice blast resistance gene Pit. Pit confers race-specific resistance against the fungal pathogen Magnaporthe grisea, and is a member of the nucleotide-binding site leucine-rich repeat (NBS-LRR) family of R genes. Compared with the non-functional allele Pit(Npb), the functional allele Pit(K59) contains four amino acid substitutions, and has the LTR retrotransposon Renovator inserted upstream. Pathogenesis assays using chimeric constructs carrying the various regions of Pit(K59) and Pit(Npb) suggest that amino acid substitutions might have a potential effect in Pit resistance; more importantly, the upregulated promoter activity conferred by the Renovator sequence is essential for Pit function. Our data suggest that transposon-mediated transcriptional activation may play an important role in the refunctionalization of additional 'sleeping' R genes in the plant genome. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/18808453/Refunctionalization_of_the_ancient_rice_blast_disease_resistance_gene_Pit_by_the_recruitment_of_a_retrotransposon_as_a_promoter_ L2 - https://doi.org/10.1111/j.1365-313X.2008.03694.x DB - PRIME DP - Unbound Medicine ER -