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The stress responsive and morphologically regulated hsp90 gene from Paracoccidioides brasiliensis is essential to cell viability.
BMC Microbiol. 2008 Sep 22; 8:158.BM

Abstract

BACKGROUND

Paracoccidioides brasiliensis is a dimorphic fungus that causes the most prevalent systemic mycosis in Latin America. The response to heat shock is involved in pathogenesis, as this pathogen switches from mycelium to yeast forms in a temperature dependent fashion that is essential to establish infection. HSP90 is a molecular chaperone that helps in the folding and stabilization of selected polypeptides. HSP90 family members have been shown to present important roles in fungi, especially in the pathogenic species, as an immunodominant antigen and also as a potential antifungal therapeutic target.

RESULTS

In this work, we decided to further study the Pbhsp90 gene, its expression and role in cell viability because it plays important roles in fungal physiology and pathogenesis. Thus, we have sequenced a Pbhsp90 cDNA and shown that this gene is present on the genome as a single copy. We have also confirmed its preferential expression in the yeast phase and its overexpression during dimorphic transition and oxidative stress. Treatment of the yeast with the specific HSP90 inhibitors geldanamycin and radicicol inhibited growth at 2 and 10 microM, respectively.

CONCLUSION

The data confirm that the Pbhsp90 gene encodes a morphologically regulated and stress-responsive protein whose function is essential to cell viability of this pathogen. This work also enforces the potential of HSP90 as a target for antifungal therapies, since the use of HSP90 inhibitors is lethal to the P. brasiliensis yeast cells in a dose-responsive manner.

Authors+Show Affiliations

Department of Cell Biology, University of Brasília, Brazil. anicola@aecom.yu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18808717

Citation

Nicola, André M., et al. "The Stress Responsive and Morphologically Regulated Hsp90 Gene From Paracoccidioides Brasiliensis Is Essential to Cell Viability." BMC Microbiology, vol. 8, 2008, p. 158.
Nicola AM, Andrade RV, Dantas AS, et al. The stress responsive and morphologically regulated hsp90 gene from Paracoccidioides brasiliensis is essential to cell viability. BMC Microbiol. 2008;8:158.
Nicola, A. M., Andrade, R. V., Dantas, A. S., Andrade, P. A., Arraes, F. B., Fernandes, L., Silva-Pereira, I., & Felipe, M. S. (2008). The stress responsive and morphologically regulated hsp90 gene from Paracoccidioides brasiliensis is essential to cell viability. BMC Microbiology, 8, 158. https://doi.org/10.1186/1471-2180-8-158
Nicola AM, et al. The Stress Responsive and Morphologically Regulated Hsp90 Gene From Paracoccidioides Brasiliensis Is Essential to Cell Viability. BMC Microbiol. 2008 Sep 22;8:158. PubMed PMID: 18808717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The stress responsive and morphologically regulated hsp90 gene from Paracoccidioides brasiliensis is essential to cell viability. AU - Nicola,André M, AU - Andrade,Rosângela V, AU - Dantas,Alessandra S, AU - Andrade,Patrícia A, AU - Arraes,Fabrício B M, AU - Fernandes,Larissa, AU - Silva-Pereira,Ildinete, AU - Felipe,Maria Sueli S, Y1 - 2008/09/22/ PY - 2008/05/01/received PY - 2008/09/22/accepted PY - 2008/9/24/pubmed PY - 2008/12/24/medline PY - 2008/9/24/entrez SP - 158 EP - 158 JF - BMC microbiology JO - BMC Microbiol. VL - 8 N2 - BACKGROUND: Paracoccidioides brasiliensis is a dimorphic fungus that causes the most prevalent systemic mycosis in Latin America. The response to heat shock is involved in pathogenesis, as this pathogen switches from mycelium to yeast forms in a temperature dependent fashion that is essential to establish infection. HSP90 is a molecular chaperone that helps in the folding and stabilization of selected polypeptides. HSP90 family members have been shown to present important roles in fungi, especially in the pathogenic species, as an immunodominant antigen and also as a potential antifungal therapeutic target. RESULTS: In this work, we decided to further study the Pbhsp90 gene, its expression and role in cell viability because it plays important roles in fungal physiology and pathogenesis. Thus, we have sequenced a Pbhsp90 cDNA and shown that this gene is present on the genome as a single copy. We have also confirmed its preferential expression in the yeast phase and its overexpression during dimorphic transition and oxidative stress. Treatment of the yeast with the specific HSP90 inhibitors geldanamycin and radicicol inhibited growth at 2 and 10 microM, respectively. CONCLUSION: The data confirm that the Pbhsp90 gene encodes a morphologically regulated and stress-responsive protein whose function is essential to cell viability of this pathogen. This work also enforces the potential of HSP90 as a target for antifungal therapies, since the use of HSP90 inhibitors is lethal to the P. brasiliensis yeast cells in a dose-responsive manner. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/18808717/The_stress_responsive_and_morphologically_regulated_hsp90_gene_from_Paracoccidioides_brasiliensis_is_essential_to_cell_viability_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-8-158 DB - PRIME DP - Unbound Medicine ER -