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Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats.
J Pharm Pharmacol 2008; 60(10):1393-402JP

Abstract

Bidens bipinnata L. is well known in China as a traditional Chinese medicine and has been used to treat hepatitis in clinics for many years. In a previous study we found that total flavonoids of Bidens bipinnata L. (TFB) had a protective effect against carbon tetrachloride (CCl4)-induced acute liver injury in mice. Now this study was designed to investigate its therapeutic effect against CCl4-induced liver fibrosis in rats and to determine, in part, its mechanism of action. The liver fibrosis model was established by subcutaneous injection of 50% CCl4 twice a week for 18 weeks. TFB (40, 80 and 160 mg kg(-1)) was administered by gastrogavage daily from the 9th week. The results showed that TFB (80 and 160 mg kg(-1)) treatment for 10 weeks significantly reduced the elevated liver index (liver weight/body weight) and spleen index (spleen weight/body weight), elevated levels of serum transaminases (alanine aminotransferase and aspartate aminotransferase), hyaluronic acid, type III procollagen and hepatic hydroxyproline. In addition, TFB markedly inhibited CCl4-induced lipid peroxidation and enhanced the activity of the antioxidant enzymes superoxide dismutase and glutathione peroxidase. Moreover, TFB (80 and 160 mg kg(-1)) treatment improved the morphologic changes of hepatic fibrosis induced by CCl4 and suppressed nuclear factor (NF)-kappaB, alpha-smooth muscle actin (SMA) protein expression and transforming growth factor (TGF)-beta1 gene expression in the liver of liver fibrosis of rats. In conclusion, TFB was able to ameliorate liver injury and protect rats from CCl4-induced liver fibrosis by suppressing oxidative stress. This process may be related to inhibiting the induction of NF-kappaB on hepatic stellate cell activation and the expression of TGF-beta1.

Authors+Show Affiliations

College of Pharmacy, Anhui Medical University, Hefei, China 230022.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18812033

Citation

Yuan, Li-Ping, et al. "Protective Effects of Total Flavonoids of Bidens Bipinnata L. Against Carbon Tetrachloride-induced Liver Fibrosis in Rats." The Journal of Pharmacy and Pharmacology, vol. 60, no. 10, 2008, pp. 1393-402.
Yuan LP, Chen FH, Ling L, et al. Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats. J Pharm Pharmacol. 2008;60(10):1393-402.
Yuan, L. P., Chen, F. H., Ling, L., Bo, H., Chen, Z. W., Li, F., ... Xia, L. J. (2008). Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats. The Journal of Pharmacy and Pharmacology, 60(10), pp. 1393-402. doi:10.1211/jpp/60.10.0016.
Yuan LP, et al. Protective Effects of Total Flavonoids of Bidens Bipinnata L. Against Carbon Tetrachloride-induced Liver Fibrosis in Rats. J Pharm Pharmacol. 2008;60(10):1393-402. PubMed PMID: 18812033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats. AU - Yuan,Li-Ping, AU - Chen,Fei-Hu, AU - Ling,Lu, AU - Bo,Hu, AU - Chen,Zhi-Wu, AU - Li,Fan, AU - Zhong,Ming-Mei, AU - Xia,Li-Juan, PY - 2008/9/25/pubmed PY - 2009/1/27/medline PY - 2008/9/25/entrez SP - 1393 EP - 402 JF - The Journal of pharmacy and pharmacology JO - J. Pharm. Pharmacol. VL - 60 IS - 10 N2 - Bidens bipinnata L. is well known in China as a traditional Chinese medicine and has been used to treat hepatitis in clinics for many years. In a previous study we found that total flavonoids of Bidens bipinnata L. (TFB) had a protective effect against carbon tetrachloride (CCl4)-induced acute liver injury in mice. Now this study was designed to investigate its therapeutic effect against CCl4-induced liver fibrosis in rats and to determine, in part, its mechanism of action. The liver fibrosis model was established by subcutaneous injection of 50% CCl4 twice a week for 18 weeks. TFB (40, 80 and 160 mg kg(-1)) was administered by gastrogavage daily from the 9th week. The results showed that TFB (80 and 160 mg kg(-1)) treatment for 10 weeks significantly reduced the elevated liver index (liver weight/body weight) and spleen index (spleen weight/body weight), elevated levels of serum transaminases (alanine aminotransferase and aspartate aminotransferase), hyaluronic acid, type III procollagen and hepatic hydroxyproline. In addition, TFB markedly inhibited CCl4-induced lipid peroxidation and enhanced the activity of the antioxidant enzymes superoxide dismutase and glutathione peroxidase. Moreover, TFB (80 and 160 mg kg(-1)) treatment improved the morphologic changes of hepatic fibrosis induced by CCl4 and suppressed nuclear factor (NF)-kappaB, alpha-smooth muscle actin (SMA) protein expression and transforming growth factor (TGF)-beta1 gene expression in the liver of liver fibrosis of rats. In conclusion, TFB was able to ameliorate liver injury and protect rats from CCl4-induced liver fibrosis by suppressing oxidative stress. This process may be related to inhibiting the induction of NF-kappaB on hepatic stellate cell activation and the expression of TGF-beta1. SN - 0022-3573 UR - https://www.unboundmedicine.com/medline/citation/18812033/Protective_effects_of_total_flavonoids_of_Bidens_bipinnata_L__against_carbon_tetrachloride_induced_liver_fibrosis_in_rats_ L2 - https://doi.org/10.1211/jpp/60.10.0016 DB - PRIME DP - Unbound Medicine ER -