Tags

Type your tag names separated by a space and hit enter

Anti-inflammatory effect of miglustat in bronchial epithelial cells.
J Cyst Fibros. 2008 Nov; 7(6):555-65.JC

Abstract

The role of CFTR deficiency in promoting inflammation remains unclear. Perez et al. [A. Perez, A.C. Issler, C.U. Cotton, T.J. Kelley, A.S. Verkman and P.B. Davis, CFTR inhibition mimics the cystic fibrosis inflammatory profile. Am J Physiol Lung Cell Mol Physiol 2007; 292:L383-L395.] recently demonstrated that the inhibition of function of w/t CFTR produces an inflammatory profile that resembles that observed in CF patients, whereas we found that correction of F508del-CFTR function with MPB-07 down-modulates the inflammatory response to P. aeruginosa in CF bronchial cells [M.C. Dechecchi, E. Nicolis, V. Bezzerri, A. Vella, M. Colombatti, B.M. Assael, et al., MPB-07 reduces the inflammatory response to Pseudomonas aeruginosa in cystic fibrosis bronchial cells. Am J Respir Cell Mol Biol 2007; 36, 615-624.]. Since both evidence support a link between CFTR function and inflammation, we extended our investigation to other F508del-CFTR correctors, such as miglustat (Norez, 2006), an approved drug for Gaucher disease, in comparison with the galactose analogue NB-DGJ. We report here that miglustat but not NB-DGJ restores F508del-CFTR function in CF bronchial epithelial IB3-1 and CuFi-1 cells. Miglustat and NB-DGJ reduce the inflammatory response to P. aeruginosa in both CF and non-CF bronchial cells, indicating that the anti-inflammatory effect is independent of the correction of F508del-CFTR function. Miglustat also inhibits the inflammatory response induced by the supernatant of mucopurulent material obtained from the lower airway tract of cystic fibrosis patients with chronic bacterial colonization (Ribeiro, 2005). Both compounds do not interfere with the adherence of P. aeruginosa to the cells and reduce the expression of IL-8 not only after challenge with P. aeruginosa but also after exposure to TNF alpha or IL-1 beta, suggesting an effect on transduction proteins downstream and in common with different receptors for pathogens. Finally, miglustat has no major effects on overall binding activity of transcription factors NF-kappaBNF-kB and AP-1. Since miglustat is an approved drug, it could be investigated as a novel anti-inflammatory molecule to ameliorate lung inflammation in CF patients.

Authors+Show Affiliations

Laboratory of Molecular Pathology, Laboratory of Clinical Chemistry and Haematology, University Hospital of Verona, Verona, Italy. cristina.dechecchi@azosp.vr.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18815075

Citation

Dechecchi, Maria Cristina, et al. "Anti-inflammatory Effect of Miglustat in Bronchial Epithelial Cells." Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, vol. 7, no. 6, 2008, pp. 555-65.
Dechecchi MC, Nicolis E, Norez C, et al. Anti-inflammatory effect of miglustat in bronchial epithelial cells. J Cyst Fibros. 2008;7(6):555-65.
Dechecchi, M. C., Nicolis, E., Norez, C., Bezzerri, V., Borgatti, M., Mancini, I., Rizzotti, P., Ribeiro, C. M., Gambari, R., Becq, F., & Cabrini, G. (2008). Anti-inflammatory effect of miglustat in bronchial epithelial cells. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 7(6), 555-65. https://doi.org/10.1016/j.jcf.2008.06.002
Dechecchi MC, et al. Anti-inflammatory Effect of Miglustat in Bronchial Epithelial Cells. J Cyst Fibros. 2008;7(6):555-65. PubMed PMID: 18815075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of miglustat in bronchial epithelial cells. AU - Dechecchi,Maria Cristina, AU - Nicolis,Elena, AU - Norez,Caroline, AU - Bezzerri,Valentino, AU - Borgatti,Monica, AU - Mancini,Irene, AU - Rizzotti,Paolo, AU - Ribeiro,Carla M P, AU - Gambari,Roberto, AU - Becq,Frederic, AU - Cabrini,Giulio, Y1 - 2008/09/23/ PY - 2008/03/04/received PY - 2008/06/06/revised PY - 2008/06/26/accepted PY - 2008/9/26/pubmed PY - 2009/3/4/medline PY - 2008/9/26/entrez SP - 555 EP - 65 JF - Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society JO - J. Cyst. Fibros. VL - 7 IS - 6 N2 - The role of CFTR deficiency in promoting inflammation remains unclear. Perez et al. [A. Perez, A.C. Issler, C.U. Cotton, T.J. Kelley, A.S. Verkman and P.B. Davis, CFTR inhibition mimics the cystic fibrosis inflammatory profile. Am J Physiol Lung Cell Mol Physiol 2007; 292:L383-L395.] recently demonstrated that the inhibition of function of w/t CFTR produces an inflammatory profile that resembles that observed in CF patients, whereas we found that correction of F508del-CFTR function with MPB-07 down-modulates the inflammatory response to P. aeruginosa in CF bronchial cells [M.C. Dechecchi, E. Nicolis, V. Bezzerri, A. Vella, M. Colombatti, B.M. Assael, et al., MPB-07 reduces the inflammatory response to Pseudomonas aeruginosa in cystic fibrosis bronchial cells. Am J Respir Cell Mol Biol 2007; 36, 615-624.]. Since both evidence support a link between CFTR function and inflammation, we extended our investigation to other F508del-CFTR correctors, such as miglustat (Norez, 2006), an approved drug for Gaucher disease, in comparison with the galactose analogue NB-DGJ. We report here that miglustat but not NB-DGJ restores F508del-CFTR function in CF bronchial epithelial IB3-1 and CuFi-1 cells. Miglustat and NB-DGJ reduce the inflammatory response to P. aeruginosa in both CF and non-CF bronchial cells, indicating that the anti-inflammatory effect is independent of the correction of F508del-CFTR function. Miglustat also inhibits the inflammatory response induced by the supernatant of mucopurulent material obtained from the lower airway tract of cystic fibrosis patients with chronic bacterial colonization (Ribeiro, 2005). Both compounds do not interfere with the adherence of P. aeruginosa to the cells and reduce the expression of IL-8 not only after challenge with P. aeruginosa but also after exposure to TNF alpha or IL-1 beta, suggesting an effect on transduction proteins downstream and in common with different receptors for pathogens. Finally, miglustat has no major effects on overall binding activity of transcription factors NF-kappaBNF-kB and AP-1. Since miglustat is an approved drug, it could be investigated as a novel anti-inflammatory molecule to ameliorate lung inflammation in CF patients. SN - 1569-1993 UR - https://www.unboundmedicine.com/medline/citation/18815075/Anti_inflammatory_effect_of_miglustat_in_bronchial_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1569-1993(08)00092-1 DB - PRIME DP - Unbound Medicine ER -