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Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography-mass spectrometry for determination of amphetamines in urine.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 15; 874(1-2):115-8.JC

Abstract

An approach using microwave-assisted derivatization (MAD) following solid-phase extraction (SPE) combined with gas chromatography-mass spectrometry (GC-MS) was developed to determine amphetamines in urine samples. The parameters affecting the derivatization efficiency - including microwave power and irradiation time - were investigated. Besides, solvent is thought critically important to MAD. Derivatization performance was studied using various solvents and compared with the performance obtained without solvent. Derivatization efficiency was clearly found to be enhanced by the presence of solvent. The highest derivatization efficiencies were obtained in ethyl acetate (EA) under microwave power of 250W for 1min. Calibration curves for all amphetamines were linear over a range from 1 to 1000ng/mL, with correlation coefficients above 0.9992. The intra-day and inter-day precision were less than 15%. The applicability of the method was tested by analyzing amphetamine-abusing subjects urine samples. Accordingly, the solvent-enhanced MAD-GC-MS method appears to be adequate for determining amphetamines in urine.

Authors+Show Affiliations

Department of Chemistry, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18815079

Citation

Chung, Li-Wen, et al. "Solvent-enhanced Microwave-assisted Derivatization Following Solid-phase Extraction Combined With Gas Chromatography-mass Spectrometry for Determination of Amphetamines in Urine." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 874, no. 1-2, 2008, pp. 115-8.
Chung LW, Liu GJ, Li ZG, et al. Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography-mass spectrometry for determination of amphetamines in urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;874(1-2):115-8.
Chung, L. W., Liu, G. J., Li, Z. G., Chang, Y. Z., & Lee, M. R. (2008). Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography-mass spectrometry for determination of amphetamines in urine. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 874(1-2), 115-8. https://doi.org/10.1016/j.jchromb.2008.09.003
Chung LW, et al. Solvent-enhanced Microwave-assisted Derivatization Following Solid-phase Extraction Combined With Gas Chromatography-mass Spectrometry for Determination of Amphetamines in Urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 15;874(1-2):115-8. PubMed PMID: 18815079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography-mass spectrometry for determination of amphetamines in urine. AU - Chung,Li-Wen, AU - Liu,Geng-Jhih, AU - Li,Zu-Guang, AU - Chang,Yan-Zin, AU - Lee,Maw-Rong, Y1 - 2008/09/07/ PY - 2008/07/03/received PY - 2008/09/01/revised PY - 2008/09/03/accepted PY - 2008/9/26/pubmed PY - 2008/12/17/medline PY - 2008/9/26/entrez SP - 115 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 874 IS - 1-2 N2 - An approach using microwave-assisted derivatization (MAD) following solid-phase extraction (SPE) combined with gas chromatography-mass spectrometry (GC-MS) was developed to determine amphetamines in urine samples. The parameters affecting the derivatization efficiency - including microwave power and irradiation time - were investigated. Besides, solvent is thought critically important to MAD. Derivatization performance was studied using various solvents and compared with the performance obtained without solvent. Derivatization efficiency was clearly found to be enhanced by the presence of solvent. The highest derivatization efficiencies were obtained in ethyl acetate (EA) under microwave power of 250W for 1min. Calibration curves for all amphetamines were linear over a range from 1 to 1000ng/mL, with correlation coefficients above 0.9992. The intra-day and inter-day precision were less than 15%. The applicability of the method was tested by analyzing amphetamine-abusing subjects urine samples. Accordingly, the solvent-enhanced MAD-GC-MS method appears to be adequate for determining amphetamines in urine. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18815079/Solvent_enhanced_microwave_assisted_derivatization_following_solid_phase_extraction_combined_with_gas_chromatography_mass_spectrometry_for_determination_of_amphetamines_in_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00664-8 DB - PRIME DP - Unbound Medicine ER -