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Comparison of antigenemia assay and semiquantitative polymerase chain reaction test for monitoring active cytomegalovirus infection in allogeneic hematopoietic cell transplant recipients.
Exp Clin Transplant. 2008 Jun; 6(2):149-54.EC

Abstract

OBJECTIVES

We sought to compare the antigenemia assay and in-house semiquantitative polymerase chain reaction to monitor human cytomegalovirus infection after transplant in hematopoietic cell transplant recipients.

MATERIALS AND METHODS

A pp65 antigen test for polymorphonuclear leukocytes and a semiquantitative polymerase chain reaction for whole blood were performed for 201 samples obtained from 26 hematopoietic cell transplant recipients over a 3-month surveillance period.

RESULTS

Fourteen episodes of antigenemia positivity were detected in 7 patients in whom human cytomegalovirus DNA loads and pp65-positive cells ranged between < 102 to 2.96 x 104 copies/mL and 0-35/ 5 x 104 polymorphonuclear leukocytes, respectively. A significant correlation was detected between human cytomegalovirus DNA load and the antigenemia test. A receiver operating characteristic analysis determined 5000 copies/mL of human cytomegalovirus as the threshold value for initiation of ganciclovir therapy.

CONCLUSIONS

Based on a comparison of the pp65 antigenemia assay, quantification of human cytomegalovirus DNA in whole blood can be used to guide clinical management of hematopoietic cell transplant recipients. This approach may have important advantages including superior sensitivity and efficient monitoring of preemptive therapy, allowing inclusion of kinetic criteria in clinical guidelines. Furthermore, a high human cytomegalovirus load among patients with grade II-IV acute graft-versus-host disease may indicate a high risk of human cytomegalovirus disease among hematopoietic cell transplant patients. Human cytomegalovirus reactivation must be monitored using more-sensitive assays such as real-time polymerase chain reaction.

Authors+Show Affiliations

Microbiology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. om24pa@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18816243

Citation

Pajand, O, et al. "Comparison of Antigenemia Assay and Semiquantitative Polymerase Chain Reaction Test for Monitoring Active Cytomegalovirus Infection in Allogeneic Hematopoietic Cell Transplant Recipients." Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation, vol. 6, no. 2, 2008, pp. 149-54.
Pajand O, Ziyaeyan M, Mousavi S, et al. Comparison of antigenemia assay and semiquantitative polymerase chain reaction test for monitoring active cytomegalovirus infection in allogeneic hematopoietic cell transplant recipients. Exp Clin Transplant. 2008;6(2):149-54.
Pajand, O., Ziyaeyan, M., Mousavi, S., Fatolahzadeh, B., Hojabri, Z., Bahador, A., & Abdossamadi, Z. (2008). Comparison of antigenemia assay and semiquantitative polymerase chain reaction test for monitoring active cytomegalovirus infection in allogeneic hematopoietic cell transplant recipients. Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation, 6(2), 149-54.
Pajand O, et al. Comparison of Antigenemia Assay and Semiquantitative Polymerase Chain Reaction Test for Monitoring Active Cytomegalovirus Infection in Allogeneic Hematopoietic Cell Transplant Recipients. Exp Clin Transplant. 2008;6(2):149-54. PubMed PMID: 18816243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of antigenemia assay and semiquantitative polymerase chain reaction test for monitoring active cytomegalovirus infection in allogeneic hematopoietic cell transplant recipients. AU - Pajand,O, AU - Ziyaeyan,M, AU - Mousavi,Sa, AU - Fatolahzadeh,B, AU - Hojabri,Z, AU - Bahador,A, AU - Abdossamadi,Z, PY - 2008/9/26/pubmed PY - 2008/11/14/medline PY - 2008/9/26/entrez SP - 149 EP - 54 JF - Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation JO - Exp Clin Transplant VL - 6 IS - 2 N2 - OBJECTIVES: We sought to compare the antigenemia assay and in-house semiquantitative polymerase chain reaction to monitor human cytomegalovirus infection after transplant in hematopoietic cell transplant recipients. MATERIALS AND METHODS: A pp65 antigen test for polymorphonuclear leukocytes and a semiquantitative polymerase chain reaction for whole blood were performed for 201 samples obtained from 26 hematopoietic cell transplant recipients over a 3-month surveillance period. RESULTS: Fourteen episodes of antigenemia positivity were detected in 7 patients in whom human cytomegalovirus DNA loads and pp65-positive cells ranged between < 102 to 2.96 x 104 copies/mL and 0-35/ 5 x 104 polymorphonuclear leukocytes, respectively. A significant correlation was detected between human cytomegalovirus DNA load and the antigenemia test. A receiver operating characteristic analysis determined 5000 copies/mL of human cytomegalovirus as the threshold value for initiation of ganciclovir therapy. CONCLUSIONS: Based on a comparison of the pp65 antigenemia assay, quantification of human cytomegalovirus DNA in whole blood can be used to guide clinical management of hematopoietic cell transplant recipients. This approach may have important advantages including superior sensitivity and efficient monitoring of preemptive therapy, allowing inclusion of kinetic criteria in clinical guidelines. Furthermore, a high human cytomegalovirus load among patients with grade II-IV acute graft-versus-host disease may indicate a high risk of human cytomegalovirus disease among hematopoietic cell transplant patients. Human cytomegalovirus reactivation must be monitored using more-sensitive assays such as real-time polymerase chain reaction. SN - 1304-0855 UR - https://www.unboundmedicine.com/medline/citation/18816243/Comparison_of_antigenemia_assay_and_semiquantitative_polymerase_chain_reaction_test_for_monitoring_active_cytomegalovirus_infection_in_allogeneic_hematopoietic_cell_transplant_recipients_ DB - PRIME DP - Unbound Medicine ER -