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Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column.
Biomed Chromatogr. 2009 Jan; 23(1):63-70.BC

Abstract

A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.

Authors+Show Affiliations

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18816506

Citation

Fu, Yu-Jie, et al. "Simultaneous Determination of Main Taxoids in Taxus Needles Extracts By Solid-phase Extraction-high-performance Liquid Chromatography With Pentafluorophenyl Column." Biomedical Chromatography : BMC, vol. 23, no. 1, 2009, pp. 63-70.
Fu YJ, Sun R, Zu YG, et al. Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column. Biomed Chromatogr. 2009;23(1):63-70.
Fu, Y. J., Sun, R., Zu, Y. G., Li, S. M., Liu, W., Efferth, T., Gu, C. B., Zhang, L., & Luo, H. (2009). Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column. Biomedical Chromatography : BMC, 23(1), 63-70. https://doi.org/10.1002/bmc.1085
Fu YJ, et al. Simultaneous Determination of Main Taxoids in Taxus Needles Extracts By Solid-phase Extraction-high-performance Liquid Chromatography With Pentafluorophenyl Column. Biomed Chromatogr. 2009;23(1):63-70. PubMed PMID: 18816506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of main taxoids in Taxus needles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column. AU - Fu,Yu-Jie, AU - Sun,Rui, AU - Zu,Yuan-Gang, AU - Li,Shuang-Ming, AU - Liu,Wei, AU - Efferth,Thomas, AU - Gu,Cheng-Bo, AU - Zhang,Lin, AU - Luo,Hao, PY - 2008/9/26/entrez PY - 2008/9/26/pubmed PY - 2009/2/24/medline SP - 63 EP - 70 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 23 IS - 1 N2 - A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/18816506/Simultaneous_determination_of_main_taxoids_in_Taxus_needles_extracts_by_solid_phase_extraction_high_performance_liquid_chromatography_with_pentafluorophenyl_column_ L2 - https://doi.org/10.1002/bmc.1085 DB - PRIME DP - Unbound Medicine ER -