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Chemical-sensory characterization of dairy manure odor using headspace solid-phase microextraction and multidimensional gas chromatography mass spectrometry-olfactometry.
J Air Waste Manag Assoc. 2008 Sep; 58(9):1187-97.JA

Abstract

Livestock operations are associated with emissions of odor, gases, and particulate matter. The majority of previous livestock odor studies focused on swine operations whereas relatively few relate to dairy cattle. Identifying the compounds responsible for the primary odor impact is a demanding analytical challenge because many critical odor components are frequently present at very low concentrations within a complex matrix of numerous insignificant volatiles. The objective of this study was to describe a chemical-sensory profile of dairy manure odor using headspace solid-phase microextraction (HS-SPME) and multidimensional gas chromatography-mass spectrometry-olfactometry (MDGC-MS-O). Two analytical approaches were used: (1) HS-SPME time-series extractions (from seconds up to 20 hr) followed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O) analyses, and (2) relatively short HS-SPME extractions (30 min) followed by MDGC-MS-O analyses on selected chromatogram heart-cuts. Dairy manure was collected at research dairy farms in the United States and Israel. Volatile organic compounds (VOCs) resolved from multiple analyses included sulfur-containing compounds, volatile fatty acids, ketones, esters, and phenol/indole derivatives. A total of 86 potential odorants were identified. Of them, 17 compounds were detected by the human nose only. A greater number of VOCs and odorous compounds were detected, as well as higher mass loading, on solid-phase microextraction (SPME) fibers observed for longer extractions with SPME. However, besides sulfur-containing compounds, other selected compounds showed no apparent competition and displacement on the SPME fiber. The use of MDGC-MS-O increased chromatographic resolution even at relatively short extractions and revealed 22 additional odorants in one of the regions of the chromatogram. The two analytical approaches were found to be parallel to some extent whereas MDGC-MS-O can also be considered as a complementary approach by resolving more detailed chemical-sensory odor profiles.

Authors+Show Affiliations

Agricultural Research Organization, Institute of Soil, Water, and Environmental Sciences, Newe Ya'ar Research Center, Ramat Yishay, Israel. laor@agri.gov.ilNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18817111

Citation

Laor, Yael, et al. "Chemical-sensory Characterization of Dairy Manure Odor Using Headspace Solid-phase Microextraction and Multidimensional Gas Chromatography Mass Spectrometry-olfactometry." Journal of the Air & Waste Management Association (1995), vol. 58, no. 9, 2008, pp. 1187-97.
Laor Y, Koziel JA, Cai L, et al. Chemical-sensory characterization of dairy manure odor using headspace solid-phase microextraction and multidimensional gas chromatography mass spectrometry-olfactometry. J Air Waste Manag Assoc. 2008;58(9):1187-97.
Laor, Y., Koziel, J. A., Cai, L., & Ravid, U. (2008). Chemical-sensory characterization of dairy manure odor using headspace solid-phase microextraction and multidimensional gas chromatography mass spectrometry-olfactometry. Journal of the Air & Waste Management Association (1995), 58(9), 1187-97.
Laor Y, et al. Chemical-sensory Characterization of Dairy Manure Odor Using Headspace Solid-phase Microextraction and Multidimensional Gas Chromatography Mass Spectrometry-olfactometry. J Air Waste Manag Assoc. 2008;58(9):1187-97. PubMed PMID: 18817111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical-sensory characterization of dairy manure odor using headspace solid-phase microextraction and multidimensional gas chromatography mass spectrometry-olfactometry. AU - Laor,Yael, AU - Koziel,Jacek A, AU - Cai,Lingshuang, AU - Ravid,Uzi, PY - 2008/9/27/pubmed PY - 2008/11/15/medline PY - 2008/9/27/entrez SP - 1187 EP - 97 JF - Journal of the Air & Waste Management Association (1995) JO - J Air Waste Manag Assoc VL - 58 IS - 9 N2 - Livestock operations are associated with emissions of odor, gases, and particulate matter. The majority of previous livestock odor studies focused on swine operations whereas relatively few relate to dairy cattle. Identifying the compounds responsible for the primary odor impact is a demanding analytical challenge because many critical odor components are frequently present at very low concentrations within a complex matrix of numerous insignificant volatiles. The objective of this study was to describe a chemical-sensory profile of dairy manure odor using headspace solid-phase microextraction (HS-SPME) and multidimensional gas chromatography-mass spectrometry-olfactometry (MDGC-MS-O). Two analytical approaches were used: (1) HS-SPME time-series extractions (from seconds up to 20 hr) followed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O) analyses, and (2) relatively short HS-SPME extractions (30 min) followed by MDGC-MS-O analyses on selected chromatogram heart-cuts. Dairy manure was collected at research dairy farms in the United States and Israel. Volatile organic compounds (VOCs) resolved from multiple analyses included sulfur-containing compounds, volatile fatty acids, ketones, esters, and phenol/indole derivatives. A total of 86 potential odorants were identified. Of them, 17 compounds were detected by the human nose only. A greater number of VOCs and odorous compounds were detected, as well as higher mass loading, on solid-phase microextraction (SPME) fibers observed for longer extractions with SPME. However, besides sulfur-containing compounds, other selected compounds showed no apparent competition and displacement on the SPME fiber. The use of MDGC-MS-O increased chromatographic resolution even at relatively short extractions and revealed 22 additional odorants in one of the regions of the chromatogram. The two analytical approaches were found to be parallel to some extent whereas MDGC-MS-O can also be considered as a complementary approach by resolving more detailed chemical-sensory odor profiles. SN - 1096-2247 UR - https://www.unboundmedicine.com/medline/citation/18817111/Chemical_sensory_characterization_of_dairy_manure_odor_using_headspace_solid_phase_microextraction_and_multidimensional_gas_chromatography_mass_spectrometry_olfactometry_ L2 - https://www.tandfonline.com/doi/full/10.3155/1047-3289.58.9.1187 DB - PRIME DP - Unbound Medicine ER -