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Apoptosis induced by diallyl disulfide in human breast cancer cell line MCF-7.
Acta Pharmacol Sin 2008; 29(10):1233-9AP

Abstract

AIM

To investigate the effect of diallyl disulfide (DADS), a component of garlic, on apoptosis in human mammary cancer cell line (MCF-7) and its mechanisms.

METHODS

Cytotoxicity was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assays. Morphology of apoptotic cells was detected by acridine orange and ethidium bromide staining. Apoptotic cells stained with propidium iodide were examined using flow cytometry. Protein levels were detected by Western blot analysis.

RESULTS

DADS inhibited the proliferation of MCF-7 cells and induced the apoptotic ratio to increase rapidly. Cleavage of the caspase-3 and caspase-3 substrate poly(ADP-ribose) polymerase was observed in MCF-7 cells after 24 h of treatment with DADS. When the MCF-7 cells were treated with 200 micromol x L DADS, the stress-activated protein kinase extracellular signal-regulated kinase (ERK), a mitogen-activated protein kinase, was inhibited after 6 h; c-Jun N-terminal kinase (JNK), that is stress-activated protein kinase (SAPK), and p38 mitogen-activated protein kinase were activated after 6 h.

CONCLUSION

These results suggest that DADS both inhibits the proliferation of MCF-7 cells and induces apoptosis of MCF-7 cells. The mechanisms may include the inhibition of ERK and the activation of the SAPK/JNK and p38 pathways.

Authors+Show Affiliations

Institute of Pharmacy and Pharmacology, College of Science and Technology, University of South China, Hengyang 421001, China. lei_xiaoyong@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18817629

Citation

Lei, Xiao-yong, et al. "Apoptosis Induced By Diallyl Disulfide in Human Breast Cancer Cell Line MCF-7." Acta Pharmacologica Sinica, vol. 29, no. 10, 2008, pp. 1233-9.
Lei XY, Yao SQ, Zu XY, et al. Apoptosis induced by diallyl disulfide in human breast cancer cell line MCF-7. Acta Pharmacol Sin. 2008;29(10):1233-9.
Lei, X. Y., Yao, S. Q., Zu, X. Y., Huang, Z. X., Liu, L. J., Zhong, M., ... Liao, D. F. (2008). Apoptosis induced by diallyl disulfide in human breast cancer cell line MCF-7. Acta Pharmacologica Sinica, 29(10), pp. 1233-9. doi:10.1111/j.1745-7254.2008.00851.x.
Lei XY, et al. Apoptosis Induced By Diallyl Disulfide in Human Breast Cancer Cell Line MCF-7. Acta Pharmacol Sin. 2008;29(10):1233-9. PubMed PMID: 18817629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis induced by diallyl disulfide in human breast cancer cell line MCF-7. AU - Lei,Xiao-yong, AU - Yao,Shu-qiong, AU - Zu,Xu-yu, AU - Huang,Ze-xiang, AU - Liu,Li-juan, AU - Zhong,Miao, AU - Zhu,Bing-yang, AU - Tang,Sheng-song, AU - Liao,Duan-fang, PY - 2008/9/27/pubmed PY - 2009/5/1/medline PY - 2008/9/27/entrez SP - 1233 EP - 9 JF - Acta pharmacologica Sinica JO - Acta Pharmacol. Sin. VL - 29 IS - 10 N2 - AIM: To investigate the effect of diallyl disulfide (DADS), a component of garlic, on apoptosis in human mammary cancer cell line (MCF-7) and its mechanisms. METHODS: Cytotoxicity was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assays. Morphology of apoptotic cells was detected by acridine orange and ethidium bromide staining. Apoptotic cells stained with propidium iodide were examined using flow cytometry. Protein levels were detected by Western blot analysis. RESULTS: DADS inhibited the proliferation of MCF-7 cells and induced the apoptotic ratio to increase rapidly. Cleavage of the caspase-3 and caspase-3 substrate poly(ADP-ribose) polymerase was observed in MCF-7 cells after 24 h of treatment with DADS. When the MCF-7 cells were treated with 200 micromol x L DADS, the stress-activated protein kinase extracellular signal-regulated kinase (ERK), a mitogen-activated protein kinase, was inhibited after 6 h; c-Jun N-terminal kinase (JNK), that is stress-activated protein kinase (SAPK), and p38 mitogen-activated protein kinase were activated after 6 h. CONCLUSION: These results suggest that DADS both inhibits the proliferation of MCF-7 cells and induces apoptosis of MCF-7 cells. The mechanisms may include the inhibition of ERK and the activation of the SAPK/JNK and p38 pathways. SN - 1745-7254 UR - https://www.unboundmedicine.com/medline/citation/18817629/Apoptosis_induced_by_diallyl_disulfide_in_human_breast_cancer_cell_line_MCF_7_ L2 - http://dx.doi.org/10.1111/j.1745-7254.2008.00851.x DB - PRIME DP - Unbound Medicine ER -