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Rapid-resolution liquid chromatography/mass spectrometry for determination and quantitation of polyphenols in grape berries.
Rapid Commun Mass Spectrom. 2008 Oct; 22(20):3089-99.RC

Abstract

A rapid-resolution liquid chromatography/mass spectrometric (RRLC/MS) method for detection and quantitation of polyphenols in grape berry skins and seeds has been developed. Pulp-free berry skins were treated with liquid nitrogen and ground; seeds were also ground. Then, 3 g of samples were extracted with 30 mL of a mixture of methanol/water/formic acid 70:30:1 (v/v/v) under sonication and 1 microL of the final extract was injected into two 100 x 2.1 mm i.d., 1.8 microm Zorbax Eclipse plus C18 columns connected in series. Compounds were fractionated using a gradient elution of acidified acetonitrile/methanol 50:50 (v/v)/water. Columns were thermostatted at 70 degrees C. MS was carried out on an Agilent 6410 QqQ instrument equipped with an electrospray ionization source. Positive and negative MS/MS product ion scans were used for compound identification, whereas positive full scan MS in the m/z range 200-1400 was used for quantitation. By means of mass spectra comparison, various flavonols, flavan-3-ols, anthocyanins and stilbenes were identified. Quantitation was performed by external calibration, and concentration values were corrected for matrix effect that was evaluated in separate experiments. Semi-quantitative estimation was performed for compounds for which standards were not commercially available. Recoveries ranged from 90-102% with relative standard deviation (RSD) <5%, whereas the between samples RSD was in the range 4-12%. Two surrogate standards were used for quality control. The developed method was applied to analyze the polyphenol content of three Vitis vinifera table cultivars at physiological maturity and after proper preservation for 6 weeks. Results demonstrated that during preservation about half of the polyphenol content was lost.

Authors+Show Affiliations

Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Roma, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18819110

Citation

Cavaliere, Chiara, et al. "Rapid-resolution Liquid Chromatography/mass Spectrometry for Determination and Quantitation of Polyphenols in Grape Berries." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 20, 2008, pp. 3089-99.
Cavaliere C, Foglia P, Gubbiotti R, et al. Rapid-resolution liquid chromatography/mass spectrometry for determination and quantitation of polyphenols in grape berries. Rapid Commun Mass Spectrom. 2008;22(20):3089-99.
Cavaliere, C., Foglia, P., Gubbiotti, R., Sacchetti, P., Samperi, R., & Laganà, A. (2008). Rapid-resolution liquid chromatography/mass spectrometry for determination and quantitation of polyphenols in grape berries. Rapid Communications in Mass Spectrometry : RCM, 22(20), 3089-99. https://doi.org/10.1002/rcm.3705
Cavaliere C, et al. Rapid-resolution Liquid Chromatography/mass Spectrometry for Determination and Quantitation of Polyphenols in Grape Berries. Rapid Commun Mass Spectrom. 2008;22(20):3089-99. PubMed PMID: 18819110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid-resolution liquid chromatography/mass spectrometry for determination and quantitation of polyphenols in grape berries. AU - Cavaliere,Chiara, AU - Foglia,Patrizia, AU - Gubbiotti,Riccardo, AU - Sacchetti,Paolo, AU - Samperi,Roberto, AU - Laganà,Aldo, PY - 2008/9/27/pubmed PY - 2008/12/17/medline PY - 2008/9/27/entrez SP - 3089 EP - 99 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 20 N2 - A rapid-resolution liquid chromatography/mass spectrometric (RRLC/MS) method for detection and quantitation of polyphenols in grape berry skins and seeds has been developed. Pulp-free berry skins were treated with liquid nitrogen and ground; seeds were also ground. Then, 3 g of samples were extracted with 30 mL of a mixture of methanol/water/formic acid 70:30:1 (v/v/v) under sonication and 1 microL of the final extract was injected into two 100 x 2.1 mm i.d., 1.8 microm Zorbax Eclipse plus C18 columns connected in series. Compounds were fractionated using a gradient elution of acidified acetonitrile/methanol 50:50 (v/v)/water. Columns were thermostatted at 70 degrees C. MS was carried out on an Agilent 6410 QqQ instrument equipped with an electrospray ionization source. Positive and negative MS/MS product ion scans were used for compound identification, whereas positive full scan MS in the m/z range 200-1400 was used for quantitation. By means of mass spectra comparison, various flavonols, flavan-3-ols, anthocyanins and stilbenes were identified. Quantitation was performed by external calibration, and concentration values were corrected for matrix effect that was evaluated in separate experiments. Semi-quantitative estimation was performed for compounds for which standards were not commercially available. Recoveries ranged from 90-102% with relative standard deviation (RSD) <5%, whereas the between samples RSD was in the range 4-12%. Two surrogate standards were used for quality control. The developed method was applied to analyze the polyphenol content of three Vitis vinifera table cultivars at physiological maturity and after proper preservation for 6 weeks. Results demonstrated that during preservation about half of the polyphenol content was lost. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18819110/Rapid_resolution_liquid_chromatography/mass_spectrometry_for_determination_and_quantitation_of_polyphenols_in_grape_berries_ L2 - https://doi.org/10.1002/rcm.3705 DB - PRIME DP - Unbound Medicine ER -