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[S-nitrosoglutathione induced nitrosative stress in yeast: modifying role of catalases].
Ukr Biokhim Zh (1999). 2008 Mar-Apr; 80(2):106-13.UB

Abstract

Possible role of catalases in modification of yeast Saccharomyces cerevisiae response to nitrosative stress was studied in the work. Yeast cell of a wild strain and catalase-defective strains were treated with nitric oxide donor S-nitrosoglutathione, then the cell survival rate, and the levels of protein carbonyls and oxidized glutathione were measured. It was shown that S-nitrosoglutathione decreased cells viability of wild and catalase-defective strains. Unexpected, yeast cells defective by both catalases survived successfully as compared with the cells of the wild strain. The intensity of stress was evaluated by measures of oxidative protein modifications and glutathione oxidation. Treatment with S-nitrosoglutathione did not affect the level of protein carbonyls and was lower by about 14 i 22 % in the cells of double mutant strains after treatments with S-nitrosoglutathione in concentrations of 10 and 20 mM. S-nitrosoglutathione induced a strong increase of the level of oxidized glutathione in yeast cells of the wild strain. This stress slightly increased the level of oxidized glutathione in the yeast cells defective by peroxisomal or both catalases. It is interesting, that an increase of oxidized glutathione level was not observed in the yeast cells defective by cytosolic catalase. The obtained results prove that catalases can modify yeast cell response to the nitrosative stress.

Authors

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Pub Type(s)

Journal Article

Language

ukr

PubMed ID

18819381

Citation

Lushchak, O, et al. "[S-nitrosoglutathione Induced Nitrosative Stress in Yeast: Modifying Role of Catalases]." Ukrains'kyi Biokhimichnyi Zhurnal (1999), vol. 80, no. 2, 2008, pp. 106-13.
Lushchak O, Lozins'kyĭ O, Nazarchuk T, et al. [S-nitrosoglutathione induced nitrosative stress in yeast: modifying role of catalases]. Ukr Biokhim Zh (1999). 2008;80(2):106-13.
Lushchak, O., Lozins'kyĭ, O., Nazarchuk, T., & Lushchak, V. (2008). [S-nitrosoglutathione induced nitrosative stress in yeast: modifying role of catalases]. Ukrains'kyi Biokhimichnyi Zhurnal (1999), 80(2), 106-13.
Lushchak O, et al. [S-nitrosoglutathione Induced Nitrosative Stress in Yeast: Modifying Role of Catalases]. Ukr Biokhim Zh (1999). 2008 Mar-Apr;80(2):106-13. PubMed PMID: 18819381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [S-nitrosoglutathione induced nitrosative stress in yeast: modifying role of catalases]. AU - Lushchak,O, AU - Lozins'kyĭ,O, AU - Nazarchuk,T, AU - Lushchak,V, PY - 2008/9/30/pubmed PY - 2008/12/17/medline PY - 2008/9/30/entrez SP - 106 EP - 13 JF - Ukrains'kyi biokhimichnyi zhurnal (1999) JO - Ukr Biokhim Zh (1999) VL - 80 IS - 2 N2 - Possible role of catalases in modification of yeast Saccharomyces cerevisiae response to nitrosative stress was studied in the work. Yeast cell of a wild strain and catalase-defective strains were treated with nitric oxide donor S-nitrosoglutathione, then the cell survival rate, and the levels of protein carbonyls and oxidized glutathione were measured. It was shown that S-nitrosoglutathione decreased cells viability of wild and catalase-defective strains. Unexpected, yeast cells defective by both catalases survived successfully as compared with the cells of the wild strain. The intensity of stress was evaluated by measures of oxidative protein modifications and glutathione oxidation. Treatment with S-nitrosoglutathione did not affect the level of protein carbonyls and was lower by about 14 i 22 % in the cells of double mutant strains after treatments with S-nitrosoglutathione in concentrations of 10 and 20 mM. S-nitrosoglutathione induced a strong increase of the level of oxidized glutathione in yeast cells of the wild strain. This stress slightly increased the level of oxidized glutathione in the yeast cells defective by peroxisomal or both catalases. It is interesting, that an increase of oxidized glutathione level was not observed in the yeast cells defective by cytosolic catalase. The obtained results prove that catalases can modify yeast cell response to the nitrosative stress. UR - https://www.unboundmedicine.com/medline/citation/18819381/[S_nitrosoglutathione_induced_nitrosative_stress_in_yeast:_modifying_role_of_catalases]_ L2 - https://www.yeastgenome.org/reference/18819381 DB - PRIME DP - Unbound Medicine ER -