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Inhibitory effect of olive oil on fibrosis induced by carbon tetrachloride in rat liver.
Clin Nutr 2008; 27(6):900-7CN

Abstract

BACKGROUND & AIMS

In this study, the inhibitory effect of olive oil on liver fibrosis induced by carbon tetrachloride (CCl(4)) has been investigated in rats.

METHODS

Rats were divided randomly into four groups: control, CCl(4), and two olive oil groups. Except for rats in the control group, all rats were orally administered CCl(4) twice a week for 8 weeks. Rats in the olive oil groups were treated daily with olive oil (2 or 10 ml/kg) through gastrogavage for the entire experimental period.

RESULTS

RT-PCR analysis showed that CCl(4) increased the hepatic mRNA expressions of lipopolysaccharide binding protein, CD14, Toll-like receptor-4, NADPH oxidase, nuclear factor-kappa beta, collagen (alpha1) (I), collagen (alpha1) (III), and transforming growth factor beta1. The expression of these mRNAs could be decreased by olive oil treatment. In addition, Western blot analysis also supported these results. CCl(4)-induced liver damage, as characterized by the increase in hepatic malondialdehyde and hydroxyproline levels. Olive oil treatment decreased the hepatic malondialdehyde and hydroxyproline levels. Histological evaluations showed that olive oil could attenuate the liver fibrosis, necrosis, and expression of smooth muscle alpha-actin that are induced by CCl(4).

CONCLUSION

It is speculated that the phenolic compounds in olive oil significantly reduced CCl(4)-induced hepatic fibrosis in rats.

Authors+Show Affiliations

Graduate Institute of Chinese Pharmaceutical Science, China Medical University, Taichung, Taiwan, ROC.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18824281

Citation

Fang, Hsun-Lang, et al. "Inhibitory Effect of Olive Oil On Fibrosis Induced By Carbon Tetrachloride in Rat Liver." Clinical Nutrition (Edinburgh, Scotland), vol. 27, no. 6, 2008, pp. 900-7.
Fang HL, Lai JT, Lin WC. Inhibitory effect of olive oil on fibrosis induced by carbon tetrachloride in rat liver. Clin Nutr. 2008;27(6):900-7.
Fang, H. L., Lai, J. T., & Lin, W. C. (2008). Inhibitory effect of olive oil on fibrosis induced by carbon tetrachloride in rat liver. Clinical Nutrition (Edinburgh, Scotland), 27(6), pp. 900-7. doi:10.1016/j.clnu.2008.08.004.
Fang HL, Lai JT, Lin WC. Inhibitory Effect of Olive Oil On Fibrosis Induced By Carbon Tetrachloride in Rat Liver. Clin Nutr. 2008;27(6):900-7. PubMed PMID: 18824281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of olive oil on fibrosis induced by carbon tetrachloride in rat liver. AU - Fang,Hsun-Lang, AU - Lai,Jinn-Tsyy, AU - Lin,Wen-Chuan, Y1 - 2008/09/27/ PY - 2008/03/07/received PY - 2008/07/10/revised PY - 2008/08/08/accepted PY - 2008/10/1/pubmed PY - 2009/2/25/medline PY - 2008/10/1/entrez SP - 900 EP - 7 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 27 IS - 6 N2 - BACKGROUND & AIMS: In this study, the inhibitory effect of olive oil on liver fibrosis induced by carbon tetrachloride (CCl(4)) has been investigated in rats. METHODS: Rats were divided randomly into four groups: control, CCl(4), and two olive oil groups. Except for rats in the control group, all rats were orally administered CCl(4) twice a week for 8 weeks. Rats in the olive oil groups were treated daily with olive oil (2 or 10 ml/kg) through gastrogavage for the entire experimental period. RESULTS: RT-PCR analysis showed that CCl(4) increased the hepatic mRNA expressions of lipopolysaccharide binding protein, CD14, Toll-like receptor-4, NADPH oxidase, nuclear factor-kappa beta, collagen (alpha1) (I), collagen (alpha1) (III), and transforming growth factor beta1. The expression of these mRNAs could be decreased by olive oil treatment. In addition, Western blot analysis also supported these results. CCl(4)-induced liver damage, as characterized by the increase in hepatic malondialdehyde and hydroxyproline levels. Olive oil treatment decreased the hepatic malondialdehyde and hydroxyproline levels. Histological evaluations showed that olive oil could attenuate the liver fibrosis, necrosis, and expression of smooth muscle alpha-actin that are induced by CCl(4). CONCLUSION: It is speculated that the phenolic compounds in olive oil significantly reduced CCl(4)-induced hepatic fibrosis in rats. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/18824281/Inhibitory_effect_of_olive_oil_on_fibrosis_induced_by_carbon_tetrachloride_in_rat_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(08)00150-7 DB - PRIME DP - Unbound Medicine ER -