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Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants.
RNA. 2008 Nov; 14(11):2305-13.RNA

Abstract

Production of aberrant messenger ribonucleoprotein particles (mRNPs) is subject to quality control (QC). In yeast strains carrying mutations of the THO complex, transcription induction triggers a number of interconnected QC phenotypes: (1) rapid degradation of several mRNAs; (2) retention of a fraction of THO-dependent mRNAs in transcription site-associated foci; and (3) formation of a high molecular weight DNA/protein complex in the 3'-ends of THO target genes. Here, we demonstrate that the 3'-5' exonucleolytic domain of the nuclear exosome factor Rrp6p is necessary for establishing all QC phenotypes associated with THO mutations. The N terminus of Rrp6p is also important presumably through its binding to the Rrp6p co-factor Rrp47p. Interestingly, the 3'-5' exonucleolytic activity of Dis3p, the only other active exonuclease of the nuclear exosome, can also contribute to RNA QC in THO mutants, while other nuclear 3'-5' exonucleases cannot. Our data show that exonucleolytic attack by the nuclear exosome is needed both for provoking mRNP QC and for its ensuing elimination of faulty RNA.

Authors+Show Affiliations

Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, The Faculty of Science, Aarhus University, DK-8000 Aarhus C, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18824516

Citation

Assenholt, Jannie, et al. "Exonucleolysis Is Required for Nuclear mRNA Quality Control in Yeast THO Mutants." RNA (New York, N.Y.), vol. 14, no. 11, 2008, pp. 2305-13.
Assenholt J, Mouaikel J, Andersen KR, et al. Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants. RNA. 2008;14(11):2305-13.
Assenholt, J., Mouaikel, J., Andersen, K. R., Brodersen, D. E., Libri, D., & Jensen, T. H. (2008). Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants. RNA (New York, N.Y.), 14(11), 2305-13. https://doi.org/10.1261/rna.1108008
Assenholt J, et al. Exonucleolysis Is Required for Nuclear mRNA Quality Control in Yeast THO Mutants. RNA. 2008;14(11):2305-13. PubMed PMID: 18824516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants. AU - Assenholt,Jannie, AU - Mouaikel,John, AU - Andersen,Kasper R, AU - Brodersen,Ditlev E, AU - Libri,Domenico, AU - Jensen,Torben Heick, Y1 - 2008/09/29/ PY - 2008/10/1/pubmed PY - 2008/11/15/medline PY - 2008/10/1/entrez SP - 2305 EP - 13 JF - RNA (New York, N.Y.) JO - RNA VL - 14 IS - 11 N2 - Production of aberrant messenger ribonucleoprotein particles (mRNPs) is subject to quality control (QC). In yeast strains carrying mutations of the THO complex, transcription induction triggers a number of interconnected QC phenotypes: (1) rapid degradation of several mRNAs; (2) retention of a fraction of THO-dependent mRNAs in transcription site-associated foci; and (3) formation of a high molecular weight DNA/protein complex in the 3'-ends of THO target genes. Here, we demonstrate that the 3'-5' exonucleolytic domain of the nuclear exosome factor Rrp6p is necessary for establishing all QC phenotypes associated with THO mutations. The N terminus of Rrp6p is also important presumably through its binding to the Rrp6p co-factor Rrp47p. Interestingly, the 3'-5' exonucleolytic activity of Dis3p, the only other active exonuclease of the nuclear exosome, can also contribute to RNA QC in THO mutants, while other nuclear 3'-5' exonucleases cannot. Our data show that exonucleolytic attack by the nuclear exosome is needed both for provoking mRNP QC and for its ensuing elimination of faulty RNA. SN - 1469-9001 UR - https://www.unboundmedicine.com/medline/citation/18824516/Exonucleolysis_is_required_for_nuclear_mRNA_quality_control_in_yeast_THO_mutants_ L2 - http://www.rnajournal.org/cgi/pmidlookup?view=long&pmid=18824516 DB - PRIME DP - Unbound Medicine ER -