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Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7.
Infect Immun. 2008 Dec; 76(12):5466-77.II

Abstract

The production of Shiga toxin (Stx) (verocytotoxin) is a major virulence factor of Escherichia coli O157:H7 strains (Shiga toxin-producing E. coli [STEC] O157). Two types of Shiga toxins, designated Stx1 and Stx2, are produced in STEC O157. Variants of the Stx2 type (Stx2, Stx2c) are associated with high virulences of these strains for humans. A bacteriophage designated 2851 from a human STEC O157 encoding the Stx2c variant was described previously. Nucleotide sequence analysis of the phage 2851 genome revealed 75 predicted coding sequences and indicated a mosaic structure typical for lambdoid phages. Analyses of free phages and K-12 phage 2851 lysogens revealed that upon excision from the bacterial chromosome, the loss of a phage-encoded IS629 element leads to fusion of phage antA and antB genes, with the generation of a recombined antAB gene encoding a strong antirepressor. In wild-type E. coli O157 as well as in K-12 strains, phage 2851 was found to be integrated in the sbcB locus. Additionally, phage 2851 carries an open reading frame which encodes an OspB-like type III effector similar to that found in Shigella spp. Investigation of 39 stx(2c) E. coli O157 strains revealed that all except 1 were positive for most phage 2851-specific genes and possessed a prophage with the same border sequences integrated into the sbcB locus. Phage 2851-specific sequences were absent from most stx(2c)-negative E. coli O157 strains, and we suggest that phage 2851-like phages contributed significantly to the dissemination of the Stx2c variant toxin within this group of E. coli.

Authors+Show Affiliations

Molecular Diagnostics and Genetics, Department of Biological Safety, Federal Institute for Risk Assessment (BfR), 12277 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18824528

Citation

Strauch, Eckhard, et al. "Bacteriophage 2851 Is a Prototype Phage for Dissemination of the Shiga Toxin Variant Gene 2c in Escherichia Coli O157:H7." Infection and Immunity, vol. 76, no. 12, 2008, pp. 5466-77.
Strauch E, Hammerl JA, Konietzny A, et al. Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infect Immun. 2008;76(12):5466-77.
Strauch, E., Hammerl, J. A., Konietzny, A., Schneiker-Bekel, S., Arnold, W., Goesmann, A., Pühler, A., & Beutin, L. (2008). Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. Infection and Immunity, 76(12), 5466-77. https://doi.org/10.1128/IAI.00875-08
Strauch E, et al. Bacteriophage 2851 Is a Prototype Phage for Dissemination of the Shiga Toxin Variant Gene 2c in Escherichia Coli O157:H7. Infect Immun. 2008;76(12):5466-77. PubMed PMID: 18824528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacteriophage 2851 is a prototype phage for dissemination of the Shiga toxin variant gene 2c in Escherichia coli O157:H7. AU - Strauch,Eckhard, AU - Hammerl,Jens Andre, AU - Konietzny,Antje, AU - Schneiker-Bekel,Susanne, AU - Arnold,Walter, AU - Goesmann,Alexander, AU - Pühler,Alfred, AU - Beutin,Lothar, Y1 - 2008/09/29/ PY - 2008/10/1/pubmed PY - 2008/12/31/medline PY - 2008/10/1/entrez SP - 5466 EP - 77 JF - Infection and immunity JO - Infect Immun VL - 76 IS - 12 N2 - The production of Shiga toxin (Stx) (verocytotoxin) is a major virulence factor of Escherichia coli O157:H7 strains (Shiga toxin-producing E. coli [STEC] O157). Two types of Shiga toxins, designated Stx1 and Stx2, are produced in STEC O157. Variants of the Stx2 type (Stx2, Stx2c) are associated with high virulences of these strains for humans. A bacteriophage designated 2851 from a human STEC O157 encoding the Stx2c variant was described previously. Nucleotide sequence analysis of the phage 2851 genome revealed 75 predicted coding sequences and indicated a mosaic structure typical for lambdoid phages. Analyses of free phages and K-12 phage 2851 lysogens revealed that upon excision from the bacterial chromosome, the loss of a phage-encoded IS629 element leads to fusion of phage antA and antB genes, with the generation of a recombined antAB gene encoding a strong antirepressor. In wild-type E. coli O157 as well as in K-12 strains, phage 2851 was found to be integrated in the sbcB locus. Additionally, phage 2851 carries an open reading frame which encodes an OspB-like type III effector similar to that found in Shigella spp. Investigation of 39 stx(2c) E. coli O157 strains revealed that all except 1 were positive for most phage 2851-specific genes and possessed a prophage with the same border sequences integrated into the sbcB locus. Phage 2851-specific sequences were absent from most stx(2c)-negative E. coli O157 strains, and we suggest that phage 2851-like phages contributed significantly to the dissemination of the Stx2c variant toxin within this group of E. coli. SN - 1098-5522 UR - https://www.unboundmedicine.com/medline/citation/18824528/Bacteriophage_2851_is_a_prototype_phage_for_dissemination_of_the_Shiga_toxin_variant_gene_2c_in_Escherichia_coli_O157:H7_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=18824528 DB - PRIME DP - Unbound Medicine ER -