[Studies on fingerprints of Bupleurum chinense and vinegar-baked Bupleurum chinense from Hubei province].Zhong Yao Cai. 2008 May; 31(5):657-9.ZY
OBJECTIVE
To establish the HPLC Fingerprint of Bupleurum chinense from GAP provenance of Hubei province Baokang, and discuss the changes before and after processing.
METHODS
Using HPLC detection method equipped with Hypersil ODS column (5 microm, 4.6 mm x 250 mm), using acetonitrile-water as mobile phase gradient elution, flow as 1.0 ml/min, UV detector set at 203 nm, column temperature was at 25 degrees C.
RESULTS
Similarity of processed Bupleurum chinense and Bupleurum chinense exceeded 0.95, and there were 13 characteristic fingerprints of processed Bupleurum chinense, 15 characteristic fingerprints of Bupleurum chinense.
CONCLUSION
The HPLC Fingerprint of Bupleurum chinense from GAP provenance of Hubei province Baokang are established, and the difference between Bupleurum chinense before and after processing is studied initially.