Tags

Type your tag names separated by a space and hit enter

Identification of quantitative trait loci for osteoarthritis of hip joints in dogs.
Am J Vet Res. 2008 Oct; 69(10):1294-300.AJ

Abstract

OBJECTIVE

To identify quantitative trait loci (QTL) associated with osteoarthritis (OA) of hip joints of dogs by use of a whole-genome microsatellite scan.

ANIMALS

116 founder, backcross, F1, and F2 dogs from a crossbred pedigree.

PROCEDURES

Necropsy scores and an optimized set of 342 microsatellite markers were used for interval mapping by means of a combined backcross and F2 design module from an online statistical program. Breed and sex were included in the model as fixed effects. Age of dog at necropsy and body weight at 8 months of age were also included in the model as covariates. The chromosomal location at which the highest F score was obtained was considered the best estimate of a QTL position. Chromosome-wide significance thresholds were determined empirically from 10,000 permutations of marker genotypes.

RESULTS

4 chromosomes contained putative QTL for OA of hip joints in dogs at the 5% chromosome-wide significance threshold: chromosomes 5, 18, 23, and 31.

CONCLUSIONS AND CLINICAL RELEVANCE

Osteoarthritis of canine hip joints is a complex disease to which many genes and environmental factors contribute. Identification of contributing QTL is a strategy to elucidate the genetic mechanisms that underlie this disease. Refinement of the putative QTL and subsequent candidate gene studies are needed to identify the genes involved in the disease process.

Links

Publisher Full Text

Authors+Show Affiliations

Mateescu RG
Department of Animal Science, College of Agricultural Sciences and Natural Resources, Oklahoma State University, Stillwater, OK 74078, USA.
Burton-Wurster NI
No affiliation info available
Tsai K
No affiliation info available
Phavaphutanon J
No affiliation info available
Zhang Z
No affiliation info available
Murphy KE
No affiliation info available
Lust G
No affiliation info available
Todhunter RJ
No affiliation info available

MeSH

AgingAnimalsChromosome MappingCrosses, GeneticDog DiseasesDogsFemaleHip JointMaleOsteoarthritisPolymorphism, GeneticQuantitative Trait Loci

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18828685

Citation

Mateescu, Raluca G., et al. "Identification of Quantitative Trait Loci for Osteoarthritis of Hip Joints in Dogs." American Journal of Veterinary Research, vol. 69, no. 10, 2008, pp. 1294-300.
Mateescu RG, Burton-Wurster NI, Tsai K, et al. Identification of quantitative trait loci for osteoarthritis of hip joints in dogs. Am J Vet Res. 2008;69(10):1294-300.
Mateescu, R. G., Burton-Wurster, N. I., Tsai, K., Phavaphutanon, J., Zhang, Z., Murphy, K. E., Lust, G., & Todhunter, R. J. (2008). Identification of quantitative trait loci for osteoarthritis of hip joints in dogs. American Journal of Veterinary Research, 69(10), 1294-300. https://doi.org/10.2460/ajvr.69.10.1294
Mateescu RG, et al. Identification of Quantitative Trait Loci for Osteoarthritis of Hip Joints in Dogs. Am J Vet Res. 2008;69(10):1294-300. PubMed PMID: 18828685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of quantitative trait loci for osteoarthritis of hip joints in dogs. AU - Mateescu,Raluca G, AU - Burton-Wurster,Nancy I, AU - Tsai,Kate, AU - Phavaphutanon,Janjira, AU - Zhang,Zhiwu, AU - Murphy,Keith E, AU - Lust,George, AU - Todhunter,Rory J, PY - 2008/10/3/pubmed PY - 2008/11/19/medline PY - 2008/10/3/entrez SP - 1294 EP - 300 JF - American journal of veterinary research JO - Am J Vet Res VL - 69 IS - 10 N2 - OBJECTIVE: To identify quantitative trait loci (QTL) associated with osteoarthritis (OA) of hip joints of dogs by use of a whole-genome microsatellite scan. ANIMALS: 116 founder, backcross, F1, and F2 dogs from a crossbred pedigree. PROCEDURES: Necropsy scores and an optimized set of 342 microsatellite markers were used for interval mapping by means of a combined backcross and F2 design module from an online statistical program. Breed and sex were included in the model as fixed effects. Age of dog at necropsy and body weight at 8 months of age were also included in the model as covariates. The chromosomal location at which the highest F score was obtained was considered the best estimate of a QTL position. Chromosome-wide significance thresholds were determined empirically from 10,000 permutations of marker genotypes. RESULTS: 4 chromosomes contained putative QTL for OA of hip joints in dogs at the 5% chromosome-wide significance threshold: chromosomes 5, 18, 23, and 31. CONCLUSIONS AND CLINICAL RELEVANCE: Osteoarthritis of canine hip joints is a complex disease to which many genes and environmental factors contribute. Identification of contributing QTL is a strategy to elucidate the genetic mechanisms that underlie this disease. Refinement of the putative QTL and subsequent candidate gene studies are needed to identify the genes involved in the disease process. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/18828685/Identification_of_quantitative_trait_loci_for_osteoarthritis_of_hip_joints_in_dogs_ L2 - https://avmajournals.avma.org/doi/10.2460/ajvr.69.10.1294?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -