Tags

Type your tag names separated by a space and hit enter

In vitro effects of phosphatidylcholine and transgalactooligosaccharides on the production of 1,2-sn-diacylglycerol by Bifidobacterium longum biovar infantis.
J Appl Microbiol. 2008 Nov; 105(5):1678-85.JA

Abstract

AIMS

To investigate the production of the tumour promoter 1,2-sn-diacylglycerol (DAG) by a human gut isolate of Bifidobacterium longum biovar infantis.

METHODS AND RESULTS

Bifidobacterium longum biovar infantis was grown in vitro using anaerobic static batch cultures in the presence of phosphatidylcholine (PC) and trans-galactooligosaccharides (TOS). Production of DAG was found to be dependent upon the presence of PC, while TOS had a reducing effect. Considerable differences in morphology, growth and metabolic end products from the micro-organism were observed under the different culture conditions.

CONCLUSIONS

Our results have provided evidence that B. longum biovar infantis can produce DAG in vitro and that a prebiotic exerted a reducing effect upon this production.

SIGNIFICANCE AND IMPACT OF THE STUDY

The results presented in this study demonstrate an ability of ostensibly beneficial member of the colonic environment to produce unwanted compounds under certain conditions. Therefore, it may be important that a combination of substrates and other factors are assessed when studying the behaviour of any bacterial group or species, especially when designing the dietary interventions.

Authors+Show Affiliations

Food Microbial Sciences Unit, Department of Food Biosciences, The University of Reading, Reading, UK. j.vulevic@reading.ac.ukNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18828790

Citation

Vulevic, J, and G R. Gibson. "In Vitro Effects of Phosphatidylcholine and Transgalactooligosaccharides On the Production of 1,2-sn-diacylglycerol By Bifidobacterium Longum Biovar Infantis." Journal of Applied Microbiology, vol. 105, no. 5, 2008, pp. 1678-85.
Vulevic J, Gibson GR. In vitro effects of phosphatidylcholine and transgalactooligosaccharides on the production of 1,2-sn-diacylglycerol by Bifidobacterium longum biovar infantis. J Appl Microbiol. 2008;105(5):1678-85.
Vulevic, J., & Gibson, G. R. (2008). In vitro effects of phosphatidylcholine and transgalactooligosaccharides on the production of 1,2-sn-diacylglycerol by Bifidobacterium longum biovar infantis. Journal of Applied Microbiology, 105(5), 1678-85. https://doi.org/10.1111/j.1365-2672.2008.03886.x
Vulevic J, Gibson GR. In Vitro Effects of Phosphatidylcholine and Transgalactooligosaccharides On the Production of 1,2-sn-diacylglycerol By Bifidobacterium Longum Biovar Infantis. J Appl Microbiol. 2008;105(5):1678-85. PubMed PMID: 18828790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro effects of phosphatidylcholine and transgalactooligosaccharides on the production of 1,2-sn-diacylglycerol by Bifidobacterium longum biovar infantis. AU - Vulevic,J, AU - Gibson,G R, Y1 - 2008/10/01/ PY - 2008/10/3/pubmed PY - 2010/2/23/medline PY - 2008/10/3/entrez SP - 1678 EP - 85 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 105 IS - 5 N2 - AIMS: To investigate the production of the tumour promoter 1,2-sn-diacylglycerol (DAG) by a human gut isolate of Bifidobacterium longum biovar infantis. METHODS AND RESULTS: Bifidobacterium longum biovar infantis was grown in vitro using anaerobic static batch cultures in the presence of phosphatidylcholine (PC) and trans-galactooligosaccharides (TOS). Production of DAG was found to be dependent upon the presence of PC, while TOS had a reducing effect. Considerable differences in morphology, growth and metabolic end products from the micro-organism were observed under the different culture conditions. CONCLUSIONS: Our results have provided evidence that B. longum biovar infantis can produce DAG in vitro and that a prebiotic exerted a reducing effect upon this production. SIGNIFICANCE AND IMPACT OF THE STUDY: The results presented in this study demonstrate an ability of ostensibly beneficial member of the colonic environment to produce unwanted compounds under certain conditions. Therefore, it may be important that a combination of substrates and other factors are assessed when studying the behaviour of any bacterial group or species, especially when designing the dietary interventions. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/18828790/In_vitro_effects_of_phosphatidylcholine_and_transgalactooligosaccharides_on_the_production_of_12_sn_diacylglycerol_by_Bifidobacterium_longum_biovar_infantis_ DB - PRIME DP - Unbound Medicine ER -